[Histonet] large fibrous bone tissue part 2

[louise renton]

Dear Cornelia & Jack

i disagree about the soaking as this will negate the chilling  effect of the
freezer. The whole idea is to have the block as cold as possible to get
maximum support from the wax*. If the tissue has been processed and
fixed properly, there should not be a necessity for "rehydrating" on water

One thing i forgot to mention wasthat when you embed, try to orientate the
tissue so that the long axis (if there is one) lies in the same direction as
the cutting stroke. when embedding, orientate the tissue at a slight
diagonal, so that the knife dous not continously pass through the tissue on
the cutting stroke - (this works well for skins also, except make sure the
dermis is away from the knife) If this doesn't make sense, let me know and i
will send a graphic privately


*ease of sectioning relies on the embedding material being as close to the
density/stiffness of the tissue being embedded. Thats why you can section
undecal bone in resin....

best regards


On Wed, May 19, 2010 at 7:33 AM, Jack Ratliff <ratliffjack <@t> hotmail.com>wrote:

> Louise has very good advice here as related to paraffin processing of this
> tissue. I may even add to soak the block a little more before taking the
> final sections. However, have you ever thought of processing into MMA resin?
> If you have these capabilities you may be very pleased with the results and
> find the microtomy less problematic. Let me know if or how I can be of
> assistance!
>
> Jack
>
>
> On May 15, 2010, at 4:30 AM, louise renton <louise.renton <@t> gmail.com>
> wrote:
>
> I have found this helps.....
>> 1. Embed the tissue in  a dep mould, as this provides more stability, then
>> 2. Face the block
>> 3.. leave in -20 deg freezer overnight
>> 4. remove from freezer and cut sections
>> 5. If you have multiple blocks to work with, leave them in the freezer
>> until
>> ready to cut
>>
>> regards
>> On Fri, May 14, 2010 at 7:42 PM, Reuel Cornelia <Reuel.Cornelia <@t> tsrh.org
>> >wrote:
>>
>> How do you process a fibrous bone tissue ( 7 mm thick).  We have use
>>> Paraffin Type 9 from Richard allan Scientific to embed works well with
>>> our
>>> bone femur( 7 mm) when cutting but on fibrous bone it does not give us a
>>> good result in cutting the blocks. It is like cutting a uterus tissue but
>>> a
>>> little bit harder. Please give me your opinion on how to remedy this kind
>>> of
>>> tissue not mentioning double embedding method or plastic. Thank you.
>>>
>>> Reuel Cornelia
>>>
>>>
>>>
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>>>
>>
>>
>> --
>> Louise Renton
>> Bone Research Unit
>> University of the Witwatersrand
>> Johannesburg
>> South Africa
>> +27 11 717 2298 (tel & fax)
>> 073 5574456 (emergencies only)
>> "There are nights when the wolves are silent and only the moon howls".
>> George Carlin
>> No trees were killed in the sending of this message.
>> However, many electrons were terribly inconvenienced.
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>>


-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.