[Histonet] IHC

Adam . anonwums1 <@t> gmail.com
Fri May 14 17:22:21 CDT 2010


I always leave my primary antibodies on overnight at 4C. I've seen some
protocols for tricky antigens where people leave it on for days. I wouldn't
leave it a room temperature though.

Go home and enjoy your Friday,
Adam

On Fri, May 14, 2010 at 4:37 PM, <sgoebel <@t> xbiotech.com> wrote:

>
>   Can  you  leave  your  primary antibody on slides overnight (or the w
> eekend)  or  will  this  screw  things  up?   I  don't want to be here
>   another couple of hours for secondary, etc. on a Friday!!
>
>
>   Sarah Goebel, B.A., HT (ASCP)
>
>   < i>H istotechnician
>
>   XBiotech USA Inc.
>
>   8201 East Riverside Dr. Bldg 4 Suit e 100
>
>   Austin, Texas  78744
>
>   (512)386-5107
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