[Histonet] IF Doublestaining

[Merced M Leiker]

I'm not sure if that was clear looking back on it:

Incubate both primaries together.
Then incubate both secondaries together.


--On Thursday, May 13, 2010 2:46 PM -0400 Merced M Leiker 
<leiker <@t> buffalo.edu> wrote:

> You could try incubating both together (primaries and then secondaries).
> I have done this before with primaries of differing isotypes. I know
> others have, too.
>
> Regards,
> Merced
>
> --On Thursday, May 13, 2010 2:00 PM -0400 Igor Deyneko
> <igor.deyneko <@t> gmail.com> wrote:
>
>> Hello Everyone!
>> I'm planning to try some IF co-staining with 2 antibodies, one is a
>> rat-anti-mouse and the other one is rabbit anti-human on a  xenograft,
>> each has an appropriate secondary, donkey anti rat and donkey
>> anti-rabbit, conjugated to 488 and 593. Can someone advise the best way
>> to perform such a procedure, I'm afraid the rules of sequential staining
>> might not work due to fluorophore instability with washes. if anyone has
>> performed such type of stain, i would appreciate any tips.
>> Thank you.
>> Igor Deyneko
>> Infinity Pharmaceuticals
>> Cambridge, MA 02139
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>
>
>
> Merced M Leiker
> Research Technician III
> Cardiovascular Medicine
> 348 Biomedical Research Building
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY 14214  USA
> leiker <@t> buffalo.edu
> 716-829-6118 (Ph)
> 716-829-2665 (Fx)
>
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.