[Histonet] IF Doublestaining

[Merced M Leiker]

You could try incubating both together (primaries and then secondaries). I 
have done this before with primaries of differing isotypes. I know others 
have, too.

Regards,
Merced

--On Thursday, May 13, 2010 2:00 PM -0400 Igor Deyneko 
<igor.deyneko <@t> gmail.com> wrote:

> Hello Everyone!
> I'm planning to try some IF co-staining with 2 antibodies, one is a
> rat-anti-mouse and the other one is rabbit anti-human on a  xenograft,
> each has an appropriate secondary, donkey anti rat and donkey anti-rabbit,
> conjugated to 488 and 593. Can someone advise the best way to perform
> such a procedure, I'm afraid the rules of sequential staining might not
> work due to fluorophore instability with washes. if anyone has performed
> such type of stain, i would appreciate any tips.
> Thank you.
> Igor Deyneko
> Infinity Pharmaceuticals
> Cambridge, MA 02139
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

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