[Histonet] histogel technique

Thu May 13 14:02:37 CDT 2010

Next day, pellet cells and resuspend them in a small amount (100-200 microliters) of Histogel or Agar.  When the gel cools cut the microcentrifuge tube open with a scalpel or razor blade.  Now you have a nice cell pellet shaped like the bottom of your centrifuge tube, and you can treat it just like any piece of tissue. Pop it in a tissue processing cassette and then to your ethanol dehydrating steps.

We use histogel routinely for clinical specimens.  We have found several things that improve the process:
1)after you pellet the cells and decant, use a cotton tip swab to pick up the last little droplet of fluid adjacent to the button.  It will hold together much better.
2)after you have added histogel, vortex and centrifuge to pellet. Put tube in ice bath, add a few drops of formalin, and wait a few minutes.  With minimal "prodding" the "plug" will essentially pour out of the tube.  No need to cut.

Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench <@t> pph.org
Voice: 760- 739-3037
Fax: 760-739-2604

-----Original Message-----
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Sent: Thursday, May 13, 2010 7:41 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [BULK] Histonet Digest, Vol 78, Issue 17

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Today's Topics:

   1. RE: FFPE cell pellet prep (JR R)
   2. xylene substitutes (Perry, Margaret)
   3. RE: AMT Cover (Jones, Laura)
   4. RE: AMT Cover (Wanda.Smith <@t> HCAhealthcare.com)
   5. RE: AMT Cover (Jennifer Anderson)
   6. RE: RE: AMT Cover (Terri Brown)
   7. RE: AMT Cover (Bell, Lynne)
   8. RE: AMT Cover (Elliott, Rachel A.)
   9. 2010 Louisiana Society for Histotechnology State Meeting	-
      Reservation Extension! (Montina Van Meter)
  10. Re: Xylene substitutes (V. Neubert)
  11. Gastric biopsies (Cristi stephenson)
  12. RE: Forwarding Request for Control Mehtod (Tony Henwood)
  13. Re: Gastric biopsies (Brandi Higgins)
  14. Re: Gastric biopsies (susanfplaza <@t> aim.com)
  15. How do I subscribe? (Michelle MacVeigh-Aloni)
  16. job opening for cytology/ FISH and histology supervisor	-
      Florida (dcojita <@t> tampabay.rr.com)
  17. RE: Gastric biopsies (Gill, Caula A.)
  18. RE: Are Histotechs considered "exempt" employees?
      (Heckford, Karen - SMMC-SF)
  19. RE: Gastric biopsies (Cynthia Pyse)
  20. Re: Gastric biopsies (Rene J Buesa)
  21. RE: Gastric biopsies (Weems, Joyce)
  22. RE: Gastric biopsies (Joyce Cline)
  23. Tilapia Fish Eye (Marquisha Paul)

----------------------------------------------------------------------

Message: 1
Date: Wed, 12 May 2010 10:36:15 -0700
From: JR R <rosenfeldtek <@t> hotmail.com>
Subject: RE: [Histonet] FFPE cell pellet prep
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY135-W681D7F121BE89CA27ACDCDBFB0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

Here's how I do it.

I hate the lens paper method.

I transfer suspended cells from a T75 flask to a 50 ml Falcon tube and centrifuge at 400 X g for 5 minutes.  Aspirate most of the media so that cells plus media are about 1 ml.  Transfer cells plus media to a 1.7 ml microcentrifuge tube.  Pellet cells at 400 x G for 5 minutes.  Rinse with PBS if needed.  Suspend cells in NBF and fix over night.  

Next day, pellet cells and resuspend them in a small amount (100-200 microliters) of Histogel or Agar.  When the gel cools cut the microcentrifuge tube open with a scalpel or razor blade.  Now you have a nice cell pellet shaped like the bottom of your centrifuge tube, and you can treat it just like any piece of tissue. Pop it in a tissue processing cassette and then to your ethanol dehydrating steps.

Jerry Ricks
Research Scientist
University of Washington
Department of Pathology

> Date: Wed, 12 May 2010 06:50:14 -0700
> From: kmerriam2003 <@t> yahoo.com
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] FFPE cell pellet prep
> 
> Sorry - I forgot to put a subject line.
> 
> 
> Good morning,
> 
> I am trying to make some nice FFPE cell pellet blocks, but I seem to lose a lot of cells along the way (especially when trying to take the cells out of the tube).  We are fixing the cells in NBF, spinning them down, adding 70% and spinning down again.  At that point, I am trying to scoop out the cells (with a weighing scoop) and wrap them in lens paper for processing.  I am losing a lot of the cells during this step, because the pellet is not quite solid.  Do you think it would be OK to let the cells air-dry a bit and then take them out for processing?  I know this goes against everything I was taught in histology, but I am really at a loss here.
> 
> Anyone have any hot tips for me?
> 
> Kim
>  Kim Merriam, MA, HT(ASCP)QIHC
> Cambridge, MA 
> 
> 
> 
>       
> _______________________________________________
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------------------------------

Message: 2
Date: Wed, 12 May 2010 12:42:57 -0500
From: "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
Subject: [Histonet] xylene substitutes
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<FCA5EF47F9BC694CBB4C58FEA04219634CD0042020 <@t> SDSU-MBX.jacks.local>
Content-Type: text/plain; charset="us-ascii"

We use formula 83 for everything and do not have a problem with a film.  We have found that we must use isopropal alcohol instead of reagent alcohol in the final dehydration steps.
Margaret Perry
SDSU

------------------------------

Message: 3
Date: Wed, 12 May 2010 14:20:36 -0400
From: "Jones, Laura" <lpjones <@t> srhs-pa.org>
Subject: [Histonet] RE: AMT Cover
To: "'Goins, Tresa'" <TGoins <@t> mt.gov>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<4AE8039AEA096143B965CBC6D0921668022CA806C5 <@t> EXCH2007.srhs-pa.org>
Content-Type: text/plain; charset="us-ascii"

What on earth were they thinking?!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Goins,
Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover

To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 4
Date: Wed, 12 May 2010 13:21:59 -0500
From: <Wanda.Smith <@t> HCAhealthcare.com>
Subject: [Histonet] RE: AMT Cover
To: <TGoins <@t> mt.gov>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<9E2D36CE2D7CBA4A94D9B22E8328A3BA138AA820C6 <@t> NADCWPMSGCMS03.hca.corpad.net>

Content-Type: text/plain; charset="us-ascii"

We felt offended by the cover also!!!!  Even my Cytotechs felt that wasn't necessary!!!
Wanda 

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover

To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 5
Date: Wed, 12 May 2010 12:07:51 -0700
From: Jennifer Anderson <janderson <@t> halozyme.com>
Subject: [Histonet] RE: AMT Cover
To: "'Wanda.Smith <@t> HCAhealthcare.com'" <Wanda.Smith <@t> HCAhealthcare.com>,
	"TGoins <@t> mt.gov" <TGoins <@t> mt.gov>, "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<5F7CC9B788911848A79BC83453A3D653040C50B25E <@t> Tomlinson.hti.com>
Content-Type: text/plain; charset="iso-8859-1"

Greetings to all.

I am SO SO happy to see that others felt the same way about the catalog cover.  I immediately went to the AMT website and gave them my opinion.  They could definitely have choreographed a more sensitive image.  I received a very nice email back, apologizing for any offense, of course un-intentional.  The advertising and media department head who sent the email assured me that they had positive feedback on the cover, in support of better techniques for cervical cancer screening.

I hope they can make better marketing decisions in the future.

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
janderson <@t> halozyme.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Wanda.Smith <@t> HCAhealthcare.com
Sent: Wednesday, May 12, 2010 11:22 AM
To: TGoins <@t> mt.gov; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: AMT Cover

We felt offended by the cover also!!!!  Even my Cytotechs felt that wasn't necessary!!!
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover

To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
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The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed.  Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege.  Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws.  If you received this in error, please contact the sender and delete/destroy this email.

------------------------------

Message: 6
Date: Wed, 12 May 2010 15:11:19 -0400
From: "Terri Brown" <Terri.Brown <@t> Northside.com>
Subject: RE: [Histonet] RE: AMT Cover
To: "Jones, Laura" <lpjones <@t> srhs-pa.org>, "Goins, Tresa"
	<TGoins <@t> mt.gov>,	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<731941C266951A47BEF11E5EFAAED9C90196E33F <@t> nsmvexch01.northside.local>
Content-Type: text/plain;charset="us-ascii"

How do we see the cover on line?  We did not receive a copy.

Terri H. Brown,, HT (ASCP)
Pathology Laboratory Manager
Northside Hospital  Atlanta
terri.brown <@t> northside.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jones,
Laura
Sent: Wednesday, May 12, 2010 2:21 PM
To: 'Goins, Tresa'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: AMT Cover

What on earth were they thinking?!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Goins,
Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover

To anyone who has received the Master Tech Flyer Volume XXXI Issue I -
please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege.

------------------------------

Message: 7
Date: Wed, 12 May 2010 15:23:59 -0400
From: "Bell, Lynne" <Lynne.Bell <@t> cvmc.org>
Subject: [Histonet] RE: AMT Cover
To: "'Goins, Tresa'" <TGoins <@t> mt.gov>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<F9E5562685AF9B498D9D8C1EC996D2440F4011BB1C <@t> cvmc-email.CVMC.ORG>
Content-Type: text/plain; charset="us-ascii"

I had this catalog on my desk and everyone that came in to Histology was astounded by the cover.  No need  for the dorsal lithotomy position!

Lynne A. Bell, HT (ASCP)
Technical Specialist, Histology
Central Vermont Medical Center
130 Fisher Road
Barre, VT  05641
802-371-4923

------------------------------

Message: 8
Date: Wed, 12 May 2010 12:35:58 -0700
From: "Elliott, Rachel A." <rachel.elliott <@t> thermofisher.com>
Subject: [Histonet] RE: AMT Cover
To: Jennifer Anderson <janderson <@t> halozyme.com>,
	"'Wanda.Smith <@t> HCAhealthcare.com'" <Wanda.Smith <@t> HCAhealthcare.com>,
	"TGoins <@t> mt.gov" <TGoins <@t> mt.gov>, "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<7B380BF8E6354F4994F20E095D43F171EF6C7458 <@t> USPHO-MXVS01.amer.thermo.com>

Content-Type: text/plain; charset="us-ascii"

So how can I see this cover?

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson
Sent: Wednesday, May 12, 2010 3:08 PM
To: 'Wanda.Smith <@t> HCAhealthcare.com'; TGoins <@t> mt.gov; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: AMT Cover

Greetings to all.

I am SO SO happy to see that others felt the same way about the catalog cover.  I immediately went to the AMT website and gave them my opinion.  They could definitely have choreographed a more sensitive image.  I received a very nice email back, apologizing for any offense, of course un-intentional.  The advertising and media department head who sent the email assured me that they had positive feedback on the cover, in support of better techniques for cervical cancer screening.

I hope they can make better marketing decisions in the future.

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
janderson <@t> halozyme.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Wanda.Smith <@t> HCAhealthcare.com
Sent: Wednesday, May 12, 2010 11:22 AM
To: TGoins <@t> mt.gov; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: AMT Cover

We felt offended by the cover also!!!!  Even my Cytotechs felt that wasn't necessary!!!
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Wednesday, May 12, 2010 12:51 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FW: AMT Cover

To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover.

Dr. Tresa Goins
Department of Livestock
Bozeman, MT 59718
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed.  Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege.  Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws.  If you received this in error, please contact the sender and delete/destroy this email.

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------------------------------

Message: 9
Date: Wed, 12 May 2010 16:39:04 -0500
From: "Montina Van Meter" <Montina.VanMeter <@t> pbrc.edu>
Subject: [Histonet] 2010 Louisiana Society for Histotechnology State
	Meeting	- Reservation Extension!
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<4FE7FB862E90E448AE32388E759220E502507C1E <@t> pbrcas31.pbrc.edu>
Content-Type: text/plain;	charset="us-ascii"

Hello Histonetters,

  The Louisiana Society for Histotechnology would like to invite you to
our annual Symposium/Conference on June 4 & 5, 2010, in Baton Rouge, LA.

  Meeting location:  The Embassy Suites Hotel

                                   414 Constitution Ave.

                                   Baton Rouge, LA  70808  

We have a block of rooms reserved for attendees at the special rate of
$99.00.  The cut-off date for reservations has been extended to May 20,
2010.   Following that date the rooms may be reserved at that rate per
availability.  The hotel will honor this rate two days prior and two
days after our meeting (please contact the Embassy Suites Hotel for
further information: 1-225-924-6566 or 1-800-Embassy).   A complimentary
hotel shuttle service is provided for those flying into Baton Rouge.
Great dining, beautiful southern plantations and gambling boats on the
Mississippi River are just a few of the attractions in the Baton Rouge
area.  Remember to ask for the Louisiana Society for Histotechnology
group when placing your reservation.  Walk-ins are always welcome!  If
you have any questions please contact:  Tina Van Meter at 225-603-0953
or vanmetmj <@t> pbrc.edu.

Workshops:

 WS #1:  FISH - IT'S NOT JUST FOR DINNER!

     Bonnie Whitaker, HT (ASCP) - sponsored by Cell Marque

     OSU Medical Center

 WS #2:  What is the Tumor Registry?

     Cynthia Boudreaux, LPN, CTR

     Touro Infirmary

 WS #3:  Veterinary vs. Clinical - Which Career is Best for Me?

     Pam Marcum, B.S., M.S., HT, (ASCP) 

     UAMS

 WS #4:  Microtomy, It's About Technique!

     Mari Ann Mailhiot, BA, HT (ASCP)

     Leica Microsystems

 WS #5:  Boot Camp for Histotechs 

     Mari Ann Mailhiot, BS, HT (ASCP)

     Leica Microsystems

 WS #6:  So You Want to Know More About Things Your Lab Doesn't Do

    Pam Marcum, B.S., M.S., HT (ASCP),  

    UAMS

    Bonnie Whitaker, HT (ASCP) QIHC

    OSU Medical Center

  WS #7:  Dilution, Titrations, and Good Pipetting in the IHC Laboratory

    Robin Simpkins, HT (ASCP)    

    Biocare Medical

  WS #8:  Detection Chemistry & Antibody Production

    Tania Ewing-Finchem, HT (ASCP)    

    Ventana Roche

Hope to see you in June!

Tina

LSH Secretary

------------------------------

Message: 10
Date: Wed, 12 May 2010 23:54:55 +0200
From: "V. Neubert" <histonet.nospam <@t> vneubert.com>
Subject: Re: [Histonet] Xylene substitutes
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4BEB23AF.3080009 <@t> vneubert.com>
Content-Type: text/plain; charset=ISO-8859-1

My hand staining setup contained some commercial naphta mix, which is
comparable to any other petroleum benzine solvant, for dewaxing.

For dehydrating/coverslipping, I used "n-Butyl acetate" a.k.a. "butyl
ethanoate". Coverslipping can be done with any xylene soluble glue.

> What are labs using for xylene substitute. I will be using it in a hand
> staining setup for Mohs. Feed back on  Histo-Clear,  Clear solve, S-3
> and formular 83.
>
> Thanks in advance.
>
> Dawn Oakes HT
>
>  
>
>
> -------------------------------------------------------------------------
> Confidentiality Notice:  This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information.  Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited.  If you are not the intended recipient, you have received this email in error.  If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication.  Also know that Internet e-mail is not secure.  In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks.   Internet messages may become corrupted, incomplete, or may incorrectly identify the sender.

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------------------------------

Message: 11
Date: Wed, 12 May 2010 14:57:36 -0700 (PDT)
From: Cristi stephenson <cls71877 <@t> sbcglobal.net>
Subject: [Histonet] Gastric biopsies
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <304545.34701.qm <@t> web81204.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific tissues.  For example, does anyone run stains to rule out H. pylori on all gastric biopsies?  Any information as to the standards in other labs would be great.
Thanks,
Cristi

------------------------------

Message: 12
Date: Thu, 13 May 2010 08:49:31 +1000
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] Forwarding Request for Control Mehtod
To: "Ian Montgomery" <ian.montgomery <@t> bio.gla.ac.uk>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <AE22DCD58FA1B045B26FF61D7259578F1CB403 <@t> x2k3.nch.kids>
Content-Type: text/plain;	charset="us-ascii"

Ah yes,

I remember, actually I don't. It was a bit of a blur!!

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ian
Montgomery
Sent: Wednesday, 12 May 2010 9:32 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: FW: [Histonet] Forwarding Request for Control Mehtod

Tony,
	Is this a universal teenage thing, filthy bedrooms? I'm
convinced there are alien life forms in my daughter's bedroom. They make
the strangest noises, grunts and groan and "I'm bored." Me, I was the
perfect teenager apart from the Celtic thing of taking to the drink and
of course, lusting after girls. Ian. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tony
Henwood
Sent: 12 May 2010 01:54
To: WILLIAM DESALVO; histonet
Subject: RE: [Histonet] Forwarding Request for Control Mehtod

Ian,

What I have done in the past is to raid the Staff Fridge (or your son's
bedroom!). There is usually old food left there that has gone moldy -
bread or even strawberries. Wearing gloves & in a fume hood slice the
bread or fruit into slices (ensuring visible growth of fungi is
present), fix for two or more days in NBF, process as usual.

Voila - fungi controls

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory
Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of WILLIAM
DESALVO
Sent: Wednesday, 12 May 2010 4:01 AM
To: histonet
Subject: [Histonet] Forwarding Request for Control Mehtod

> I wonder if you could help me find someone who would kindly donate a
> paraffin tissue block containing candida or other fungi to be used as 
> a control block. We have had difficulty in obtaining such tissue and 
> have tried all the hospitals in northern Ireland for a tissue block 
> suitable enough to act as a control. Many of the pathology departments

> are in the same position as ourselves in that the control tissue being

> used is getting in short supply.
> 
> I tried to make one using a culture from microbiology with fresh lambs

> lung from a local abattoir but unfortunately the tissue seems to stain

> with PAS as well and the candida where obscured although visible
> probably due to post mortem changes in the tissue.

> Alternatively have any of your members successfully produced fungal
> control material by other means.
> 
> Thanking you in advance

> Ian Clarke
> Biomedical Scientist
> Cellular Pathology Department
> Craigavon Area Hospital
> Sothern Health and Social Services Trust.
> 66 Lurgan Road
> Craigavon
> BT63 5QQ
> Northern Ireland

William DeSalvo, B.S., HTL(ASCP)

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------------------------------

Message: 13
Date: Wed, 12 May 2010 19:46:32 -0400
From: Brandi Higgins <brandihiggins <@t> gmail.com>
Subject: Re: [Histonet] Gastric biopsies
To: Cristi stephenson <cls71877 <@t> sbcglobal.net>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<AANLkTimd_Ff5NaFUhkpBTH-f4kVYbNBquiGhZRGtoMfp <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

We do Diff Quik on all gastric biopsies, and we do Alcian Blue on all
esophageal biopsies with Barrett's indicated by the GI Dr.  Our other
special stains are by request, but we almost always end up doing retic and
trichrome on liver bx submitted with hepatitis as Clinical Dx.  Hope this
helps.

Brandi Higgins BS, HT(ASCP)

On Wed, May 12, 2010 at 5:57 PM, Cristi stephenson
<cls71877 <@t> sbcglobal.net>wrote:

> Hello Histoland,
> I was wondering if anyone out there does stains other than H&E 's on
> specific tissues.  For example, does anyone run stains to rule out H.
> pylori on all gastric biopsies?  Any information as to the standards in
> other labs would be great.
> Thanks,
> Cristi
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

------------------------------

Message: 14
Date: Wed, 12 May 2010 21:57:10 -0400 (EDT)
From: susanfplaza <@t> aim.com
Subject: Re: [Histonet] Gastric biopsies
To: cls71877 <@t> sbcglobal.net, histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CCC056BBCA5837-89C-3C16 <@t> webmail-d041.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

We routinely do PAS with and without digestion, Trichrome and Iron on liver biopsies.  Some doctors I've worked with preferred an Alcian Yellow/Toluidine Blue or a Giemsa for H. Pylori.  We occasionally did H. Pylori using immunohistochemistry.  We also do Iron stains on all bone marrow smears (fixed in methanol) and biopsies.  Hope this helps.

Susan Plaza, HT (ASCP), QIHC

-----Original Message-----
From: Cristi stephenson <cls71877 <@t> sbcglobal.net>
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Wed, May 12, 2010 5:25 pm
Subject: [Histonet] Gastric biopsies

Hello Histoland,
 was wondering if anyone out there does stains other than H&E 's on specific 
issues.  For example, does anyone run stains to rule out H. pylori on all 
astric biopsies?  Any information as to the standards in other labs would be 
reat.
hanks,
risti
______________________________________________
istonet mailing list
istonet <@t> lists.utsouthwestern.edu
ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 15
Date: Wed, 12 May 2010 19:18:10 -0700
From: "Michelle MacVeigh-Aloni" <macveigh <@t> usc.edu>
Subject: [Histonet] How do I subscribe?
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <AD1F39A8A74644308B3A7D89F0B80BDE <@t> DFS66DD1>
Content-Type: text/plain;	charset="iso-8859-1"

Hi all,

Can someone tell me - how does one subscribe to Master Tech Flyer?

Thank you in advance
Michelle

------------------------------

Message: 16
Date: Wed, 12 May 2010 22:38:10 -0400
From: <dcojita <@t> tampabay.rr.com>
Subject: [Histonet] job opening for cytology/ FISH and histology
	supervisor	- Florida
To: "'histonet'" <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<!~!UENERkVCMDkAAQACAAAAAAAAAAAAAAAAABgAAAAAAAAAdq7zShuC4ki+F530qNQCZMKAAAAQAAAAu8QpJ2E83E+TDvJS6Kiw8QEAAAAA <@t> tampabay.rr.com>

Content-Type: text/plain;	charset="us-ascii"

I have a job opening for a cytotechnologist (non-gyn only) preferably with
experience with Urovysion/FISH (not required but willing to learn) in the
Tampa bay area. 

I also know of a job opening for a histologist; supervisory position (part
time or full time) in Ormond Beach, Florida 

Please call Diane anytime for details 813-843-0483

------------------------------

Message: 17
Date: Thu, 13 May 2010 08:05:57 -0400
From: "Gill, Caula A." <cgill <@t> marylandgeneral.org>
Subject: RE: [Histonet] Gastric biopsies
To: "Cristi stephenson" <cls71877 <@t> sbcglobal.net>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<087A9911BBAFDE4B8151CB148586E2C23A9E96 <@t> MDGEN-EXCH1.marylandgeneral.org>

Content-Type: text/plain;	charset="iso-8859-1"

Hello Cristi,

We routinely do a steiner on all Gastric Bx.'s we also do Pas W and WO digestion, Fe, Trichrome and retic on all liver with DX of Hepatitis. GMS-P on Lung bx POS. for HIV if requested by Dr. Elastic, retic and Trichrome  on Temporal Arteries.

Caula Gill (HT) ASCP

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cristi stephenson
Sent: Wednesday, May 12, 2010 5:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Gastric biopsies

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific tissues.  For example, does anyone run stains to rule out H. pylori on all gastric biopsies?  Any information as to the standards in other labs would be great.
Thanks,
Cristi
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 18
Date: Thu, 13 May 2010 05:09:34 -0700
From: "Heckford, Karen - SMMC-SF" <Karen.Heckford <@t> CHW.edu>
Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
To: "joelle weaver" <joelleweaver <@t> hotmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<2842DC75AE43AA4B92954CFB31781BC105697FAC <@t> CHW-MSG-301.chw.edu>
Content-Type: text/plain;	charset="US-ASCII"

It's just crazy the wage differences around the country.  But we should
fight for the higher wages in whatever location we live in.  Not
everyone can do this type of job and be good at it.  

Karen Heckford HT ASCP CE

Lead Histology Technician

St. Mary's Medical Center

450 Stanyan St.

San Francisco, Ca. 94117

415-668-1000 ext. 6167

________________________________

From: joelle weaver [mailto:joelleweaver <@t> hotmail.com] 
Sent: Thursday, May 13, 2010 4:24 AM
To: Heckford, Karen - SMMC-SF; histonet-bounces <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?

Boy, I wish! I live in Ohio with over 10 years bench experience,
supervisory experience, HTL and almost a masters (4 classes left)- and I
don't approach the HT scale you proposed-even the minimum.

> Date: Tue, 11 May 2010 05:15:40 -0700
> From: Karen.Heckford <@t> CHW.edu
> To: sandoval.1965 <@t> hotmail.com
> Subject: RE: [Histonet] Are Histotechs considered "exempt" employees?
> CC: histonet <@t> lists.utsouthwestern.edu
> 
> $16.15 per hour. They are getting a great deal. I believe you should
> be making at least double that. What I believe is happening is that a
> lot of offices are now starting to open their own labs and they are
> trying to get us cheap. We all need to stick to our guns and not let
> them get us cheaper than we are worth. Yes, I realize that San
> Francisco is more but the starting wage for a HT's should be $25.00 to
> $30.00 an hour to start for a newly certified tech. No matter of
> location in California. You can always negotiate higher if you have
> more experience and more certifications.
> 
> Just my two cents worth,
> 
> Karen Heckford HT ASCP CE
> Lead Histology Technician
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Anthony
> Sandoval
> Sent: Monday, May 10, 2010 9:01 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Are Histotechs considered "exempt" employees?
> 
> Hello fellow Histotechs, I have recently become certified as an HTL
and
> was 
> wondering if anyone out there is an 'exempt' employee? I live in
> California 
> and feel that I am being taken advantage of. I make 16.15$ per hour
and
> 
> frequently work 50 hour weeks. Am I off base? should I just be
grateful
> that 
> I have a job, as my employer so frequently reminds me? Thank you
> Histonet! 
> you have been an invaluable resource in my career and assisting me in 
> passing the HTL! 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

________________________________

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------------------------------

Message: 19
Date: Thu, 13 May 2010 08:37:25 -0400
From: "Cynthia Pyse" <cpyse <@t> x-celllab.com>
Subject: RE: [Histonet] Gastric biopsies
To: "'Cristi stephenson'" <cls71877 <@t> sbcglobal.net>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001caf299$0ad3a100$207ae300$@com>
Content-Type: text/plain;	charset="iso-8859-1"

Christi
We run a diff stain on all gastric bx suspected of H. pylori as routine, it
saves us time. 

Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cpyse <@t> x-celllab.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cristi
stephenson
Sent: Wednesday, May 12, 2010 5:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Gastric biopsies

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on
specific tissues.  For example, does anyone run stains to rule out H.
pylori on all gastric biopsies?  Any information as to the standards in
other labs would be great.
Thanks,
Cristi
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 20
Date: Thu, 13 May 2010 06:10:22 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Gastric biopsies
To: histonet <@t> lists.utsouthwestern.edu,	Cristi stephenson
	<cls71877 <@t> sbcglobal.net>
Message-ID: <120364.96574.qm <@t> web65704.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

For gastric biopsies we routinely did modified Steiner for H. pylori
René J.

--- On Wed, 5/12/10, Cristi stephenson <cls71877 <@t> sbcglobal.net> wrote:

From: Cristi stephenson <cls71877 <@t> sbcglobal.net>
Subject: [Histonet] Gastric biopsies
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, May 12, 2010, 5:57 PM

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific tissues.  For example, does anyone run stains to rule out H. pylori on all gastric biopsies?  Any information as to the standards in other labs would be great.
Thanks,
Cristi
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 21
Date: Thu, 13 May 2010 09:37:44 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: RE: [Histonet] Gastric biopsies
To: "susanfplaza <@t> aim.com" <susanfplaza <@t> aim.com>,
	"cls71877 <@t> sbcglobal.net"	<cls71877 <@t> sbcglobal.net>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<92AD9B20A6C38C4587A9FEBE3A30E164015DE0679E <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"

For H. pylori we do a Genta, which is a modified Steiner, with Alcian blue and H & E over it. 

I've finally gotten the docs to wait to make sure they need specials on everything else, so as not to waste. J

Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of susanfplaza <@t> aim.com
Sent: Wednesday, May 12, 2010 21:57
To: cls71877 <@t> sbcglobal.net; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Gastric biopsies

We routinely do PAS with and without digestion, Trichrome and Iron on liver biopsies.  Some doctors I've worked with preferred an Alcian Yellow/Toluidine Blue or a Giemsa for H. Pylori.  We occasionally did H. Pylori using immunohistochemistry.  We also do Iron stains on all bone marrow smears (fixed in methanol) and biopsies.  Hope this helps.

Susan Plaza, HT (ASCP), QIHC

-----Original Message-----
From: Cristi stephenson <cls71877 <@t> sbcglobal.net>
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Wed, May 12, 2010 5:25 pm
Subject: [Histonet] Gastric biopsies

Hello Histoland,
 was wondering if anyone out there does stains other than H&E 's on specific issues.  For example, does anyone run stains to rule out H. pylori on all astric biopsies?  Any information as to the standards in other labs would be reat.
hanks,
risti
______________________________________________
istonet mailing list
istonet <@t> lists.utsouthwestern.edu
ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
This email, including any attachments is the 
property of Catholic Health East and is intended 
for the sole use of the intended recipient(s).  
It may contain information that is privileged and 
confidential.  Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are 
not the intended recipient, please reply to the 
sender that you have received the message in 
error, then delete this message.

------------------------------

Message: 22
Date: Thu, 13 May 2010 09:44:33 -0400
From: Joyce Cline <Joyce.Cline <@t> wchsys.org>
Subject: RE: [Histonet] Gastric biopsies
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<FE2197D76184F4408CFEBD95C28282685BF1E1DBD9 <@t> WCHSXCHCM.wchsys.org>
Content-Type: text/plain; charset="us-ascii"

We do Iron,Trichrome, Pas with and without Diastase and Retic on liver biopsies if requested by the grossing pathologist. We do Diff-Quik on all stomach biopsies, and if requested by the pathologist an Hp immno. A tricchrome is done on colon with a diagnosis of microscopic or collagenous colitis . A pas light green is done on esophogeal biopsies for candida. Any other specials are done at the request of the pathologist.

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cristi stephenson [cls71877 <@t> sbcglobal.net]
Sent: Wednesday, May 12, 2010 5:57 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Gastric biopsies

Hello Histoland,
I was wondering if anyone out there does stains other than H&E 's on specific tissues.  For example, does anyone run stains to rule out H. pylori on all gastric biopsies?  Any information as to the standards in other labs would be great.
Thanks,
Cristi
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system.

------------------------------

Message: 23
Date: Thu, 13 May 2010 09:59:18 -0400
From: "Marquisha Paul" <MPaul <@t> Alltech.com>
Subject: [Histonet] Tilapia Fish Eye
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<421EE4FDC90A744CB1DDDDD21A1BC56E09FBB027 <@t> ERGO.Alltech-bio.com>
Content-Type: text/plain;	charset="iso-8859-1"

Dear Histonetters,

Does anyone out there have experience with processing whole tilapia fish eye?  We formalin fixed our specimen, dehydrated with EtOH and cleared with Citrisolv before embedding in paraffin wax.  At the microtome we encountered all kinds of chatter.  Any advice is greatly appreciated.

Thanks,
Marquisha Paul

------------------------------

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End of Histonet Digest, Vol 78, Issue 17
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