[Histonet] IF Doublestaining

[Igor Deyneko]

Hello Everyone!
I'm planning to try some IF co-staining with 2 antibodies, one is a
rat-anti-mouse and the other one is rabbit anti-human on a  xenograft, each
has an appropriate secondary, donkey anti rat and donkey anti-rabbit,
conjugated to 488 and 593. Can someone advise the best way to perform such a
procedure, I'm afraid the rules of sequential staining might not work due to
fluorophore instability with washes. if anyone has performed such type of
stain, i would appreciate any tips.
Thank you.
Igor Deyneko
Infinity Pharmaceuticals
Cambridge, MA 02139