FW: [Histonet] Forwarding Request for Control Mehtod

[Lynette Pavelich]

If you have one of those great automatic stainers that has a drain tube
going into a sink, you will also find that, after some time, a wonderful
fungus will grow inside of the tubing!! We remove the tubing and shake
the fungus loose.  Counter staining isn't the best on this type of
specimen, but when you're in a pinch, you will definitely obtain
positive fungus staining!

And, wherever you are, it must be warmer/dryer than Michigan right
now..........please send heat!!!

hope this helped!
Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI  48503
email: Lpaveli1 <@t> hurleymc.com
ph:  810-257-9948
Lab:  810-257-9138
fax:  810-762-7082


>>> "Ian Montgomery" <ian.montgomery <@t> bio.gla.ac.uk> 5/12/2010 7:31 AM
>>>
Tony,
	Is this a universal teenage thing, filthy bedrooms? I'm
convinced
there are alien life forms in my daughter's bedroom. They make the
strangest
noises, grunts and groan and "I'm bored." Me, I was the perfect
teenager
apart from the Celtic thing of taking to the drink and of course,
lusting
after girls.
Ian. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tony
Henwood
Sent: 12 May 2010 01:54
To: WILLIAM DESALVO; histonet
Subject: RE: [Histonet] Forwarding Request for Control Mehtod

Ian,

What I have done in the past is to raid the Staff Fridge (or your
son's
bedroom!).
There is usually old food left there that has gone moldy - bread or
even
strawberries.
Wearing gloves & in a fume hood slice the bread or fruit into slices
(ensuring visible growth of fungi is present), fix for two or more
days
in NBF, process as usual.

Voila - fungi controls

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
WILLIAM
DESALVO
Sent: Wednesday, 12 May 2010 4:01 AM
To: histonet
Subject: [Histonet] Forwarding Request for Control Mehtod



> I wonder if you could help me find someone who would kindly donate a

> paraffin tissue block containing candida or other fungi to be used as

> a control block. We have had difficulty in obtaining such tissue and

> have tried all the hospitals in northern Ireland for a tissue block 
> suitable enough to act as a control. Many of the pathology
departments

> are in the same position as ourselves in that the control tissue
being

> used is getting in short supply.
> 
> I tried to make one using a culture from microbiology with fresh
lambs

> lung from a local abattoir but unfortunately the tissue seems to
stain

> with PAS as well and the candida where obscured although visible 
> probably due to post mortem changes in the tissue.
 
> Alternatively have any of your members successfully produced fungal 
> control material by other means.
> 
> Thanking you in advance
 
> Ian Clarke
> Biomedical Scientist
> Cellular Pathology Department
> Craigavon Area Hospital
> Sothern Health and Social Services Trust.
> 66 Lurgan Road
> Craigavon
> BT63 5QQ
> Northern Ireland


William DeSalvo, B.S., HTL(ASCP)



 		 	   		  
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