[Histonet] need tips for cross-sectioning of cortical bone

[Jack Ratliff]

Why not embed in resin (MMA) and take thicker sections and then grind/ 
polish them down? If you went this route, you could then use  
flourescent labels and quantify mineral apposition rate and bone  
formation rate. Let me know if you are interested. I can help you get  
started and direct you to low cost equipment options.

Jack

On Apr 22, 2010, at 9:58 AM, "Connolly, Brett M" <brett_connolly <@t> merck.com 
 > wrote:

> A colleague is having trouble getting wrinkle-free sections of
> decalcified, paraffin embedded femur.
>
> Any tips??
>
> Thanks,
>
> Brett M. Connolly, Ph.D.
> Molecular Imaging Team Leader
> Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, PA 19486
> tel. 215-652-2501 fax. 215-993-6803
> brett_connolly <@t> merck.com
>
>
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