[Histonet] Re: Histonet Digest, Vol 78, Issue 12

[Stephanie Rodriguez]

We use this clone also.  It is distributed by Invitrogen, but the company
that makes it is CalTag. (Maybe Invitrogen owns them.)  We use AR of ph 9.0
Tris (0.5mM)/EDTA (0.1mM) for 20 min, dilution of 1:2000, and detection of
Dako Flex+.

It is not an IVD, by the way; it¹s still RUO.

Stephanie Rodriguez, HTL(ASCP), QIHC
Senior Molecular Tech/IHC Tech III
Phenopath Laboratories
Seattle, WA



On 5/10/10 10:43 AM, "histonet-request <@t> lists.utsouthwestern.edu"
<histonet-request <@t> lists.utsouthwestern.edu> wrote:

> Message: 2
> Date: Mon, 10 May 2010 13:26:20 +0200
> From: "Heather McCleod" <Heather.Mcleod <@t> uct.ac.za>
> Subject: Re: [Histonet] IgG4 source
> To: "Joao Pessoa" <jphistology <@t> gmail.com>,
>  <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <4BE8097C020000CB0003516F <@t> gwiasmtp.uct.ac.za>
> Content-Type: text/plain; charset=US-ASCII
> 
> I have used invitrogen - mouse anti-human IgG4 Clone HP6025  (successfully)
> AR in 1mm EDTA  Dilution 1:400
> Control - tonsil  (or Sjogren's Syndrome)
> 
>>>> >>> Joao Pessoa <jphistology <@t> gmail.com> 2010/05/07 11:50 PM >>>
> I need to find a source for an IgG4 antibody for use with IHC studies on
> human FFPE tissue.  Any ideas?  My preference would be for a
> concentrated IVD.
> 
> Thanks,
> 
> Joao
> Histo Tech
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 



This e-mail message, including any attachments, is for the sole use of the 
intended recipients and may contain privileged information. Any unauthorized 
review, use, disclosure or distribution is prohibited. If you are not the intended 
recipient, please contact the sender by e-mail and destroy all copies of the 
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. 
at (206) 374-9000.