SPAM-LOW: [Histonet] Loss of tissue sections after antigenretrieval

[Kelsey Jones]

Patsy is correct, there is a very good chance the retrieval is the
issue. Another possible issue is the drying time. We find often times
with tissue loss we can remedy that with extended drying times. If we
get precut slides from an outside source, we dry them for 2 hours in a
58 degree oven to be cautious. 

Kelsey Jones
Technical Consultant 

6600 Sierra College Blvd.
Rocklin, CA 95677
(916) 746-8900 x8969
(916) 746-8989 (fax)
www.cellmarque.com

**Check out Cell Marque on Facebook!
  


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy
Ruegg
Sent: Saturday, May 08, 2010 4:11 PM
To: 'Joseph N. Pastore'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: SPAM-LOW: [Histonet] Loss of tissue sections after
antigenretrieval

Joe,

Did you buy an array that was not cut with the tape transfer system and
put
onto polymer coated slides?  If they had done that your tissues would
not
fall off, not sure of what to do after the fact, but you could certainly
try
a more gentle antigen retrieval technique such as waterbath (not sure of
what you are using as your heat source?)  sounds like your retrieval
method
is too harsh.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joseph
N.
Pastore
Sent: Saturday, May 08, 2010 1:32 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] Loss of tissue sections after antigen
retrieval

Hi All,
We have a problem. We are using a commercial tissue array and we find
that
the two most important tissues are lost during antigen retrieval. The
tissues are samples of glioblastomas and are critical to our study. The
antigen retrieval system we use is a proprietary 2 step system using a
hi
and lo pH. Both Ca chelators. Does anyone have suggestions about how we
can
prevent tissue loss???? 
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