[Histonet] Loss of tissue sections after antigen retrieval

[Joseph N. Pastore]

Hi All,
We have a problem. We are using a commercial tissue array and we find that the two most important tissues are lost during antigen retrieval. The tissues are samples of glioblastomas and are critical to our study. The antigen retrieval system we use is a proprietary 2 step system using a hi and lo pH. Both Ca chelators. Does anyone have suggestions about how we can prevent tissue loss????