[Histonet] Special stain to demonstrate Silver

[Troutman, Kenneth A]

Hi Fanny,

I don't know of one, but perhaps you could modify a GMS.  I don't think you would need to oxidize it at the beginning with the periodic acid or chromic acid.  Make the working Methenamine silver solution without the silver.  (Don't forget to add the Borate) and try and visualize it that way.  I probably wouldn't do the hypo and gold chloride until I looked at it under the scope to see if it was actually staining, you wouldn't want to remove the silver you are trying to visualize.  I would be careful in interpreting that because I don't know what else it might be picking up--it might pick up other metals.  That's just a suggestion off the cuff.  If you try that, let us know what happens!  Good luck!

Thanks,

Ashley Troutman BS, HT(ASCP) QIHC
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4532
Nashville, TN  37232
615-343-9134

Message: 15
Date: Fri, 7 May 2010 07:00:25 -0500
From: "DeGuzman, Maria" <mdeguzman <@t> lifecell.com>
Subject: [Histonet] Special stain to demonstrate Silver
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Hello Histoneters,
Is there a special stain to detect silver in tissue?  If there is please share the procedure.
Thanks,
Fanny