[Histonet] mouse kidney frozen sectioning

[Michelle MacVeigh-Aloni]

Hi Andrew,

You must freeze fast to avoid the needle like crystals forming from the 
water during freezing. Best - freeze in OCT in liquid Nitrogen.
It gets worse if you keep changing the temp of the block. For example keep 
at -80 then bring to -20 cut than bring the temp down to remove the block 
from the chuck and put back at -80. Few of those will make your tissue hard 
to even recognize.

Good luck
Michelle HTL
USC Keck School of Medicine
LA, CA

----- Original Message ----- 
From: "Andrew Burgeson" <napoli <@t> siscom.net>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, May 03, 2010 12:31 PM
Subject: [Histonet] mouse kidney frozen sectioning


> Having trouble with freezing artifact in the form of tiny
> fissures or cracks in mouse kidney on frozen section.
>
> Tissue is paraformaldehyde fixed and infiltrated w 70%
> aqueous sucrose OCT solution.
>
> Anyone else seen this and know how to deal with it?
>
> Thx
>
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