[Histonet] mouse kidney frozen sectioning

Andrew Burgeson napoli <@t> siscom.net
Mon May 3 14:31:31 CDT 2010


Having trouble with freezing artifact in the form of tiny
fissures or cracks in mouse kidney on frozen section.

Tissue is paraformaldehyde fixed and infiltrated w 70%
aqueous sucrose OCT solution.

Anyone else seen this and know how to deal with it?

Thx



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