[Histonet] IHC detection for Pig tissue?
Reuel.Cornelia <@t> tsrh.org
Wed Mar 31 17:20:05 CDT 2010
I just wanted to know if anybody who are working with Pig bone tissue fix in 10%NBF and decalcified in 14% EDTA, citrate buffer (dako) antigen retrieval pH7.0 and ph 9.0 by steaming for 20 minutes, what is their IHC detection kit for mouse monoclonal and rabbit polyclonal antibodies. I am using a Powervision kit and I have a lot of background staining even with my negative staining. Please help.
Reuel Cornelia, BS MT, AMT
Texas Scottish Rite Hospital for Children
2222 Welborn Street
Dallas, TX 75219
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