FW: [Histonet] Coverslipping with SubX

[Vanessa Avalos]

Sally, 

I added SubX to my acrymount since I have plenty of that in stock. It
worked!!  And I am so glad I can use up what I have instead of having to
purchase a case of Sub X glue. My slides look clear and film free. I now am
more confident in passing these slides over to be read. 

Thanks for all the helpful suggestions everyone!

-----Original Message-----
From: Breeden, Sara [mailto:sbreeden <@t> nmda.nmsu.edu] 
Sent: Tuesday, March 30, 2010 11:25 AM
To: Vanessa Avalos
Subject: RE: [Histonet] Coverslipping with SubX

Try thinning out the Sub-X-based mounting medium with some Sub-X.  It
sounds as if your mounting medium is too thick.  Try that and see if it
helps.  You want your mounting medium to drip like cheap pancake syrup
would drip - not too thick and not too thin.  Too thick makes bubbles.

I use Sub-X on my processor but use xylene on my autostainer, but that's
because I have an tape coverslipper.  I know you mean you're
coverslipping by hand but see if the thinner medium helps you.  Let me
know!

Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM  87106
505-841-2576