[Histonet] GMS stain

Malika Benatti malbenatti <@t> googlemail.com
Wed Mar 31 14:20:29 CDT 2010

Hi Margaret,

What problem are you experiencing ?
Are you over impregnated or under impregnated ?
At what thickness do you cut your Section ? Ideally 3 to 4 microns
How do you make up you Hexamine solution ? if you get contamination of
Hexamine solution make sure that glass ware is clean.

Try this.
Make 0.75 g of hexamine in 100 ml coplin jar / leave it dissolve in
distilled H2O at 60 oC while your slides are in chromic acid. When ready
slowly ad 18 drops (approximately 1ml) of 5% silver nitrate, (make sure
solution remain clear if cloudy, then glassware is contaminated and repeat
this steps with chromic acid clean glassware) then add 2 ml of borax. Sliver
solution should remain clear. add slides and check macroscopically slide
after 5 to 7 mins, then at 5 mins interval after that. Depending on tissue,
it should not take more that 15 mins to develop, then carry to next step.

Hope this help

Best wishes


Malika Benatti BSc MIBMS
Specialist Biomedical Scientist
Great Ormond Street Hospital
London, UK

On Wed, Mar 31, 2010 at 7:46 PM, Perry, Margaret <Margaret.Perry <@t> sdstate.edu
> wrote:

> We sometimes have problems with the stain if we use positive slides.
> Margaret
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