[Histonet] RE: lab markers KP
Merced M Leiker
leiker <@t> buffalo.edu
Wed Mar 31 11:40:55 CDT 2010
I agree as well! - I use them to mark everything, even things that don't
get treated with chemicals.
One note: they work best if you mark hard non-porous surfaces (like the
plastic cassettes) the day before to give the ink time to bind with the
material.
Regards,
Merced
--On Wednesday, March 31, 2010 11:31 AM -0400 "Blazek, Linda"
<lblazek <@t> digestivespecialists.com> wrote:
> I have to agree. They have never failed and work forever.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Madary,
> Joseph Sent: Wednesday, March 31, 2010 11:30 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] lab markers KP
>
> The only consistent lab pen I have used that will hold up under decal,
> processing with various alcohol, xylene, hemo de is the KP marker plus.
> I am sure there are several vendors, I use Mercedes.
>
> Nick Madary, HT/HTL(ASCP)QIHC
> Medimmune Histology Mgr,
> OMW, Area 4, Lab 2438
> 301.398.4745(vm)
> 301.398.6360(lab)
> 301.398.9745(fax)
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> histonet-request <@t> lists.utsouthwestern.edu Sent: Wednesday, March 31, 2010
> 10:45 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 76, Issue 45
>
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>
> Today's Topics:
>
> 1. Coverslipping with SubX (Vanessa Avalos)
> 2. Haunted cryostat/Thanks! (mtitford <@t> aol.com)
> 3. GMS on decal tissue (Morken, Tim)
> 4. questions (Webb, Dorothy L)
> 5. Re: her2 validation (Pat Laurie)
> 6. FREE MONEY! (Jackie M O'Connor)
> 7. RE: questions (Garrison, Becky)
> 8. Thermo's Excelsior Tissue Processor Program's
> (Akemi Allison-Tacha)
> 9. re: cyto prep tech (Kim Tournear)
> 10. her-2 neu validation (Debbie Nannenga)
> 11. RE: questions (Tony Henwood)
> 12. RE: RE: Leica Paraplast (connie grubaugh)
> 13. (no subject) (Green JumpyOne)
> 14. Fwd: [Histonet] (no subject) (Malika Benatti)
> 15. RE: Thermo's Excelsior Tissue Processor Program's
> (Heckford, Karen - SMMC-SF)
> 16. RE: RE: Leica Paraplast (Nails, Felton)
> 17. Thermo Fisher Excelsior Tissue Processor Programs (Ann Bennett)
> 18. Texas Society for Histotechnology April 23-25, 2010
> (kdwyer3322 <@t> aol.com)
> 19. cassette labels erased by processor (Catherine Breen)
> 20. RE: her2 validation (Weems, Joyce)
> 21. RE: cassette labels erased by processor (Sherwood, Margaret )
> 22. FW: [Histonet] cassette labels erased by processor (Cheri Miller)
> 23. Re: cassette labels erased by processor (Malika Benatti)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 30 Mar 2010 11:15:38 -0700
> From: "Vanessa Avalos" <vavalos <@t> allergydermatology.com>
> Subject: [Histonet] Coverslipping with SubX
> To: "'HISTONET LISTS'" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000001cad035$00d2f5b0$0278e110$@com>
> Content-Type: text/plain; charset="us-ascii"
>
> Is anyone using SubX ? I am trying it out and am having some difficulty
> coverslipping. I am using the Subx glue as directed since Acrymount didn't
> seem to work as well. I still get a hazy film under the glass and am
> getting big air bubbles as well. I can eventually get them out but its
> just takes a while and you know how time is precious when you have a line
> of slides to stain. The process is not as smooth as before. I really
> would like this to work out for me and eliminate xylene.
>
> Any suggestions??
>
>
>
> Vanessa
>
> Fax: 602-277-2134
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 30 Mar 2010 14:44:26 -0400
> From: mtitford <@t> aol.com
> Subject: [Histonet] Haunted cryostat/Thanks!
> To: Histonet <@t> pathology.swmed.edu
> Message-ID: <8CC9E5028ADFB36-1500-2BE5 <@t> webmail-m010.sysops.aol.com>
> Content-Type: text/plain; charset="us-ascii"
>
>
>
> Thank you everyone who responded to my problem with a Leica CM 1850
> cryostat turning itself off. About 11 people responded with tips. Thank
> you!! The Histonet is great!! In answer to some enquirys:
> 1) The cryostat is set to defrost at 2 a.m.. The manual says it defrosts
> for an hour. Jackie O' Conner asks if it is set to go back "on" after the
> defrost. I have no idea. It defrosts well the rest of the time, and turns
> itself back on. Brian Cornett-Early recommends changing the start device
> on the compressor. 2) Someone else asked if power is getting to the
> cryostat - Yes, when it turns itself off, the on/off switch is on "off".
> All you have to do is flip the switch and it starts pumping and cooling.
> 3) Mari Ann Mailhiot with Leica recommends changing a cicuit breaker on
> the side of the compressor. That is probably where we will start. Mari
> Ann works for Leica so it sounds like good advice.
>
> Thank you everyone. Since it only breaks down about once a month, it will
> take a long time to determine if the problem is fixed.
>
> Michael Titford
> Pathology USA
> Mobile AL USA
>
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 30 Mar 2010 11:52:17 -0700
> From: "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>
> Subject: [Histonet] GMS on decal tissue
> To: histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <1AAF670737F193429070841C6B2ADD4C013B16C780 <@t> EXMBMCB15.ucsfmedicalcenter.o
> rg>
>
> Content-Type: text/plain; charset=us-ascii
>
> Has anyone seen or heard of problems with Grocott Methenamine Silver
> staining for fungi on decal tissues? Specifically, background or false
> positives? I can't find anything in any books that gives any indications
> to not use decaled tissue.
>
> Thanks!
>
> Tim Morken
> Supervisor, Histology / IPOX
> UCSF Medical Center
> Box 1656
> 1600 Divisadero St.
> San Francisco, CA 94143-1656
> USA
>
> Phone: (415) 514-6042
> Pager: (415) 443-6509
> Fax: (415) 885-7409
>
> Email: tim.morken <@t> ucsfmedctr.org<mailto:tim.morken <@t> ucsfmedctr.org>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 30 Mar 2010 14:03:55 -0500
> From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
> Subject: [Histonet] questions
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <65365F35C0F2EF4D846EC3CA73E49C43C5786C5846 <@t> HPEMX3.HealthPartners.int>
> Content-Type: text/plain; charset="us-ascii"
>
> We have been having problems with underprocessed placental membrane, some
> of which are cut fairly thick, but am seeing it on more than just the
> thich samples. Does anyone out there in histoland have a special process
> for placentas or any helpful hints? I do know that many times the
> placentas sit without formalin in L&D for hours before they bring them to
> histo and add formalin and this seems to me it could be a factor, even
> though we have them sitting in formalin for a few hours before processing.
>
> Also, does anyone do the high-iron diamine stain for intestinal mucin
> staining? Do you do it with an Alcian Blue-PAS stain?
>
> Thank you ahead of time for any and all responses!!
>
> Dorothy Webb, HT (ASCP)
> Regions Histology Lab
>
>
>
> ________________________________
> This e-mail and any files transmitted with it are confidential and are
> intended solely for the use of the individual or entity to whom they are
> addressed. If you are not the intended recipient or the individual
> responsible for delivering the e-mail to the intended recipient, please
> be advised that you have received this e-mail in error and that any use,
> dissemination, forwarding, printing, or copying of this e-mail is
> strictly prohibited.
>
> If you have received this e-mail in error, please immediately notify the
> HealthPartners Support Center by telephone at (952) 967-6600. You will be
> reimbursed for reasonable costs incurred in notifying us. HealthPartners
> R001.0
>
>
> ------------------------------
>
> Message: 5
> Date: Tue, 30 Mar 2010 13:26:21 -0700
> From: Pat Laurie <foreightl <@t> gmail.com>
> Subject: Re: [Histonet] her2 validation
> To: anita dudley <azdudley <@t> hotmail.com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <bdfbc2371003301326n2031c2e2v2c0a2a193196c676 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> We left it up to our pathologist. Initially we did the 25 slide
> validation, our pathologist wasn't 100% convinced that he could read them
> accurately, so we did another 25, plus all of the ones we sent out which
> already had FISH ran. It ended up being almost 70 cases, and our
> pathologist was properly "educated" on how he should score them. He felt
> that there was excessive pressure put on the pathologists due to having
> to score these slides, not just say negative or positive. I'll say
> though for the last year or so, with about 6 her2 cases ran a day, he has
> been remarkably accurate. Any that he scores 2+ and has sent out for
> FISH he usually indicates if he thinks that it will be positive or
> negative. He has yet to be wrong. Another Pathologist who I talked to
> who helped set up the guidelines said that there isn't a "1 method fits
> all" process. Good luck.
>
> On Tue, Mar 30, 2010 at 7:19 AM, anita dudley <azdudley <@t> hotmail.com>
> wrote:
>
>>
>> sorry!!! I didn't put a subject in the previous post. wondering about
>> how many slides people are using for her2 validation. thanks
>>
>>
>>
>> anita dudley
>>
>> providence hospital
>>
>> mobile alabama
>>
>> _________________________________________________________________
>> The New Busy is not the old busy. Search, chat and e-mail from your
>> inbox.
>>
>> http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:
>> ON:WL:en-US:WM_HMP:032010_3_____________________________________________
>> __ Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
> --
> Patrick Laurie HT(ASCP)QIHC
> CellNetix Pathology & Laboratories
> 1124 Columbia Street, Suite 200
> Seattle, WA 98104
> PH: 206-215-5949
> plaurie <@t> cellnetix.com
>
>
> ------------------------------
>
> Message: 6
> Date: Tue, 30 Mar 2010 15:28:30 -0500
> From: Jackie M O'Connor <Jackie.O'Connor <@t> abbott.com>
> Subject: [Histonet] FREE MONEY!
> To: histonet <@t> lists.utsouthwestern.edu,
> histonet-bounces <@t> lists.utsouthwestern.edu
> Message-ID:
>
> <OFAB0D17A8.5145E414-ON862576F6.006FF8AE-862576F6.00708491 <@t> abbott.com>
> Content-Type: text/plain; charset="US-ASCII"
>
> Now that I have your attention - -
>
> I would really like to hear from you if you are at all interested in a
> histology position at Abbott Laboratories in Northern Illinois. Even if
> you have applied previously.
>
> This is a great opportunity to work in a GLP research lab, a good deal of
> bench work, but developing IHC for toxicology purposes as well. We have
> a Biocare intelliPATH stainer - it's FUN!
>
> We have four full time technicians/technologists with a fifth open
> position. Abbott offers 3 weeks paid vacation, great benefits, and is a
> good company to work for. I've been here ten years, and I've never been
> anywhere 10 years.
>
> Please forward your resume to me, as well as go to www.Abbott.com to
> apply.
>
> Jackie O'Connor
>
>
> ------------------------------
>
> Message: 7
> Date: Tue, 30 Mar 2010 16:49:42 -0400
> From: "Garrison, Becky" <becky.garrison <@t> jax.ufl.edu>
> Subject: RE: [Histonet] questions
> To: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <B3CA1BAF6C5E4541867E4E79AD2412E006A5CFC0 <@t> jaxmail.umc.ufl.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> We receive the placentas fresh (they are refrigerated in L&D before
> transport to Pathology); add lots of formalin (use 163 oz containers)
> and let fix overnight. Early next morning, placenta is grossed and
> cassettes sit in formalin til end of day. This formalin is changed at
> least once so that bloody formalin is replaced with fresh. Placed on
> processor at end of second day after receipt.
>
> When we had L&D add formalin, there was never enough formalin added and
> the placentas sat unfixed at room temperature for long periods of time.
> This procedure works better for us. Yes, we can not meet the CAP
> guideline
> for 2 day TAT but do end up with a consistently better quality product
> for this tissue type. (Placentas make up a small portion of overall
> workload, so overall TAT is not affected). Prior to this procedure,
> placentas made
> up a disproportionate amount of reprocessed blocks.
>
> Becky Garrison
> Pathology Supervisor
> Shands Jacksonville
> Jacksonville, FL 32209
> 904-244-6237, phone
> 904-244-4290, fax
> 904-393-3194, pager
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Webb,
> Dorothy L
> Sent: Tuesday, March 30, 2010 3:04 PM
> To: 'histonet <@t> lists.utsouthwestern.edu'
> Subject: [Histonet] questions
>
> We have been having problems with underprocessed placental membrane,
> some of which are cut fairly thick, but am seeing it on more than just
> the thich samples. Does anyone out there in histoland have a special
> process for placentas or any helpful hints? I do know that many times
> the placentas sit without formalin in L&D for hours before they bring
> them to histo and add formalin and this seems to me it could be a
> factor, even though we have them sitting in formalin for a few hours
> before processing.
>
> Also, does anyone do the high-iron diamine stain for intestinal mucin
> staining? Do you do it with an Alcian Blue-PAS stain?
>
> Thank you ahead of time for any and all responses!!
>
> Dorothy Webb, HT (ASCP)
> Regions Histology Lab
>
>
>
> ________________________________
> This e-mail and any files transmitted with it are confidential and are
> intended solely for the use of the individual or entity to whom they are
> addressed. If you are not the intended recipient or the individual
> responsible for delivering the e-mail to the intended recipient, please
> be advised that you have received this e-mail in error and that any use,
> dissemination, forwarding, printing, or copying of this e-mail is
> strictly prohibited.
>
> If you have received this e-mail in error, please immediately notify the
> HealthPartners Support Center by telephone at (952) 967-6600. You will
> be reimbursed for reasonable costs incurred in notifying us.
> HealthPartners R001.0
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 8
> Date: Tue, 30 Mar 2010 16:04:19 -0700 (PDT)
> From: Akemi Allison-Tacha <akemiat3377 <@t> yahoo.com>
> Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's
> To: histo net <histonet <@t> pathology.swmed.edu>
> Message-ID: <465571.85431.qm <@t> web113812.mail.gq1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi All of you in Histoland,
>
> I am working with a facility that recently purchased a Thermo Excelsior
> Tissue Processor. They have had processing problems with several of
> their specimens using non-xylene clearing agent. These issues were with
> both bx's and larger tissues. The program and the non-xylene clearing
> agent was recommended by the technical staff at Thermo.
>
> The histology staff have switched back to using xylene verses the
> non-xylene clearing agent, and most of the issues have disappeared.
>
> Also, the histotech's were using reagent alcohol, histological grade.
> This grade of alcohol is denatured with methyl alcohol. The sales
> representative was in and informed us that this type of alcohol has
> damaging effects on the instrument. The representative will be coming in
> to flush out the system and reprogram the instrument next Monday.
>
> I looked over the current VIP program which is being used, and the
> program, timing and temperatures are a little different from most
> hospital and private laboratories I have worked with. We are going to
> use basically the same program for the Excelsior, that we use on the VIP.
>
> I would like to know what other Excelsior Tissue Processor users that use
> xylene have for their programs. It would be great to compare the
> reagents, programs, timing, and temperatures for Routine overnight runs
> and for Rapid Biopsy Runs. Thank you in advance for your assistance.
>
> Akemi Allison-Tacha BS, HT(ASCP)HTL
> Director
> Phoenix Lab Consulting
> E-Mail: akemiat3377 <@t> yahoo.com
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Tue, 30 Mar 2010 16:05:40 -0700 (PDT)
> From: Kim Tournear <kimtournear <@t> yahoo.com>
> Subject: [Histonet] re: cyto prep tech
> To: Histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <839809.46245.qm <@t> web54204.mail.re2.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Thank you to everyone who responded to my question about cyto prep tech
> work,
>
>
>
> ~Kim Tournear ~ HT (ASCP), QIHC (ASCP)
> Histology Supervisor
> Tucson Medical Center
> Tucson, AZ
> ~Don't let your life end before it begins~
> OU Rocks!!!!
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Tue, 30 Mar 2010 17:12:57 -0700
> From: Debbie Nannenga <histowa13 <@t> hotmail.com>
> Subject: [Histonet] her-2 neu validation
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <SNT118-W22EF34188ABEA72E7468D3B21E0 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> Hi Anita,
>
>
>
>
>
> We ran 25 amplified and 25 negative cases for our validation study.
>
>
>
> Good Luck.
>
>
>
> Debbie Nannenga, HTL(ASCP) QIHC
>
> InCyte Pathology
>
> Spokane, WA
>
>
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Wed, 31 Mar 2010 11:21:58 +1100
> From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
> Subject: RE: [Histonet] questions
> To: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <B9EAF61856077F47BF9BE2F89AFC555206853921 <@t> hedwig.nch.kids>
> Content-Type: text/plain; charset="us-ascii"
>
> Dorothy,
>
> Apart from hoping the blocks of tissue are not too thick, we microwave
> the cassettes in 10%NBF in a Milestone Mega TT - 2 hours at 45oC. This
> ensures adequate fixation prior to processing on a Shandon Excelsior
> Tissue Processor.
>
>
> Regards
>
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Webb,
> Dorothy L
> Sent: Wednesday, 31 March 2010 6:04 AM
> To: 'histonet <@t> lists.utsouthwestern.edu'
> Subject: [Histonet] questions
>
>
> We have been having problems with underprocessed placental membrane,
> some of which are cut fairly thick, but am seeing it on more than just
> the thich samples. Does anyone out there in histoland have a special
> process for placentas or any helpful hints? I do know that many times
> the placentas sit without formalin in L&D for hours before they bring
> them to histo and add formalin and this seems to me it could be a
> factor, even though we have them sitting in formalin for a few hours
> before processing.
>
> Also, does anyone do the high-iron diamine stain for intestinal mucin
> staining? Do you do it with an Alcian Blue-PAS stain?
>
> Thank you ahead of time for any and all responses!!
>
> Dorothy Webb, HT (ASCP)
> Regions Histology Lab
>
>
>
> ________________________________
> This e-mail and any files transmitted with it are confidential and are
> intended solely for the use of the individual or entity to whom they are
> addressed. If you are not the intended recipient or the individual
> responsible for delivering the e-mail to the intended recipient, please
> be advised that you have received this e-mail in error and that any use,
> dissemination, forwarding, printing, or copying of this e-mail is
> strictly prohibited.
>
> If you have received this e-mail in error, please immediately notify the
> HealthPartners Support Center by telephone at (952) 967-6600. You will
> be reimbursed for reasonable costs incurred in notifying us.
> HealthPartners R001.0 _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> *************************************************************************
> ******** This email and any files transmitted with it are confidential
> and intended solely for the use of the individual or entity to whom they
> are addressed. If you are not the intended recipient, please delete it
> and notify the sender.
>
> Views expressed in this message and any attachments are those of the
> individual sender, and are not necessarily the views of The Children's
> Hospital at Westmead
>
> This note also confirms that this email message has been virus scanned
> and although no computer viruses were detected, The Childrens Hospital at
> Westmead accepts no liability for any consequential damage resulting from
> email containing computer viruses.
> *************************************************************************
> ********
>
>
>
> ------------------------------
>
> Message: 12
> Date: Tue, 30 Mar 2010 19:58:21 -0700
> From: connie grubaugh <conniegrubaugh <@t> hotmail.com>
> Subject: RE: [Histonet] RE: Leica Paraplast
> To: <ddreesen <@t> sbcglobal.net>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <SNT104-W197006F3C487BD2D0A97E9D61E0 <@t> phx.gbl>
> Content-Type: text/plain; charset="Windows-1252"
>
>
> Hi all, I questioned the Leica paraplast that we have received too. It
> is real gummy and sticky. Takes me forever to cut. I asked and was
> informed that it is the same stuff we have always got and there is no
> difference. Except all of us techs have noticed a big difference.
>
>
>
> Connie G.
>
>
>
>
>
>> Date: Tue, 23 Mar 2010 11:29:03 -0700
>> From: ddreesen <@t> sbcglobal.net
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] RE: Leica Paraplast
>>
>>
>> Hi Kristen,
>> We were using the Paraplast Xtra and switched to something else after we
>> noticed a difference in the quality of the paraffin. The tissues weren't
>> cutting as well and the paraffin seemed to be "gritty". We found we were
>> going through many more blades due to nicks and scratches and many times
>> had to switch blades mid-block.
>>
>>
>> From: histonet-request <@t> lists.utsouthwestern.edu
>> <histonet-request <@t> lists.utsouthwestern.edu Message: 4
>> Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT)
>> From: kristen arvidson <arvidsonkristen <@t> yahoo.com>
>> Subject: [Histonet] Leica Paraplast
>> To: histonet <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID: <94596.93077.qm <@t> web65707.mail.ac4.yahoo.com>
>> Content-Type: text/plain; charset=iso-8859-1
>>
>> Has anyone who uses paraplast (we use the basic one) noticed a change in
>> the quality of your tissue? I have recently found out that they have
>> changed manufacturing sites in the past couple of months. I am having
>> on and off issues with my skin specimens that have been going on for
>> about 2 months or so. Thought there may be a correlation. Any thoughts?
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _________________________________________________________________
> Hotmail: Trusted email with Microsoft's powerful SPAM protection.
> http://clk.atdmt.com/GBL/go/210850552/direct/01/
>
> ------------------------------
>
> Message: 13
> Date: Wed, 31 Mar 2010 01:52:42 -0700
> From: Green JumpyOne <greenjumpyone <@t> hotmail.com>
> Subject: [Histonet] (no subject)
> To: <aga8ton <@t> live.com>, <histonet <@t> lists.utsouthwestern.edu>,
> <jkbrown2986 <@t> hotmail.com>
> Message-ID: <BLU128-W26777912678997BEEAAEB4AB1E0 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
> http://www.trainedlabor.com/H6UH4Yh1UJ.html
>
> _________________________________________________________________
> The New Busy is not the old busy. Search, chat and e-mail from your inbox.
> http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:O
> N:WL:en-US:WM_HMP:032010_3
>
> ------------------------------
>
> Message: 14
> Date: Wed, 31 Mar 2010 10:45:09 +0100
> From: Malika Benatti <malbenatti <@t> googlemail.com>
> Subject: Fwd: [Histonet] (no subject)
> To: Histonet List <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <8186A0A0-94D8-4E31-99D1-87B35FC3CDC6 <@t> gmail.com>
> Content-Type: text/plain; charset=us-ascii; format=flowed;
> delsp=yes
>
> Dear Histonet list manager
>
> Is they anyway you could filter/block spam such as this one from be
> sent out to the Histonet list.
>
> Cheers
>
> Malika Benatti BSc MIBMS
> Specialist Biomedical Scientist
> Great Ormond Street Children Hospital
> London
>
> " ... Smile it confuses people ..."
>
> Begin forwarded message:
>
>> From: Green JumpyOne <greenjumpyone <@t> hotmail.com>
>> Date: 31 March 2010 09:52:42 GMT+01:00
>> To: <aga8ton <@t> live.com>, <histonet <@t> lists.utsouthwestern.edu>,
>> <jkbrown2986 <@t> hotmail.com
>> >
>> Subject: [Histonet] (no subject)
>>
>
>> http://www.trainedlabor.com/H6UH4Yh1UJ.html
>>
>> _________________________________________________________________
>> The New Busy is not the old busy. Search, chat and e-mail from your
>> inbox.
>> http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:
>> ON:WL:en-US:WM_HMP:032010_3_____________________________________________
>> __ Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 15
> Date: Wed, 31 Mar 2010 05:02:26 -0700
> From: "Heckford, Karen - SMMC-SF" <Karen.Heckford <@t> CHW.edu>
> Subject: RE: [Histonet] Thermo's Excelsior Tissue Processor Program's
> To: "Akemi Allison-Tacha" <akemiat3377 <@t> yahoo.com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <2842DC75AE43AA4B92954CFB31781BC105697F76 <@t> CHW-MSG-301.chw.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Pretty much any problems I have had with the Excelsior and I have been
> using one for 4 years now is with Pathologists loading it incorrectly. I
> use reagent alcohol in it all the time, with no problems. I prefer
> using xylene in my processor. Every single time I have switched and
> started using a non-xylene sub. I have had nothing but problems. If I
> use a non-xylene I save it for the stainer. Take it easy,
>
> Karen Heckford HT ASCP CE
> Lead Histology Technician
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Akemi
> Allison-Tacha Sent: Tuesday, March 30, 2010 4:04 PM
> To: histo net
> Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's
>
> Hi All of you in Histoland,
>
> I am working with a facility that recently purchased a Thermo Excelsior
> Tissue Processor. They have had processing problems with several of
> their specimens using non-xylene clearing agent. These issues were with
> both bx's and larger tissues. The program and the non-xylene clearing
> agent was recommended by the technical staff at Thermo.
>
> The histology staff have switched back to using xylene verses the
> non-xylene clearing agent, and most of the issues have disappeared.
>
> Also, the histotech's were using reagent alcohol, histological grade.
> This grade of alcohol is denatured with methyl alcohol. The sales
> representative was in and informed us that this type of alcohol has
> damaging effects on the instrument. The representative will be coming in
> to flush out the system and reprogram the instrument next Monday.
>
> I looked over the current VIP program which is being used, and the
> program, timing and temperatures are a little different from most
> hospital and private laboratories I have worked with. We are going to
> use basically the same program for the Excelsior, that we use on the VIP.
>
> I would like to know what other Excelsior Tissue Processor users that use
> xylene have for their programs. It would be great to compare the
> reagents, programs, timing, and temperatures for Routine overnight runs
> and for Rapid Biopsy Runs. Thank you in advance for your assistance.
>
> Akemi Allison-Tacha BS, HT(ASCP)HTL
> Director
> Phoenix Lab Consulting
> E-Mail: akemiat3377 <@t> yahoo.com
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 16
> Date: Wed, 31 Mar 2010 07:27:34 -0500
> From: "Nails, Felton" <flnails <@t> texaschildrens.org>
> Subject: RE: [Histonet] RE: Leica Paraplast
> To: "'connie grubaugh'" <conniegrubaugh <@t> hotmail.com>,
> "ddreesen <@t> sbcglobal.net" <ddreesen <@t> sbcglobal.net>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <C1FE5960057C084CA389CE97779062904C52DEFE <@t> TCDMSG01.ad.TexasChildrensHospi
> tal.org>
>
> Content-Type: text/plain; charset=us-ascii
>
> There is about three brands of paraplast and they all cut differently. In
> one of my labs I use paraplast plus and it ribbons very well however the
> blocks have to be colder then if you are using TissuePrep from Fisher.
> Leica may have sent you one of the other types of paraplast. (paraplast,
> Paraplast Plus, Paraplast Xtra)
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of connie
> grubaugh Sent: Tuesday, March 30, 2010 9:58 PM
> To: ddreesen <@t> sbcglobal.net; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] RE: Leica Paraplast
>
>
> Hi all, I questioned the Leica paraplast that we have received too. It
> is real gummy and sticky. Takes me forever to cut. I asked and was
> informed that it is the same stuff we have always got and there is no
> difference. Except all of us techs have noticed a big difference.
>
>
>
> Connie G.
>
>
>
>
>
>> Date: Tue, 23 Mar 2010 11:29:03 -0700
>> From: ddreesen <@t> sbcglobal.net
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] RE: Leica Paraplast
>>
>>
>> Hi Kristen,
>> We were using the Paraplast Xtra and switched to something else after we
>> noticed a difference in the quality of the paraffin. The tissues weren't
>> cutting as well and the paraffin seemed to be "gritty". We found we were
>> going through many more blades due to nicks and scratches and many times
>> had to switch blades mid-block.
>>
>>
>> From: histonet-request <@t> lists.utsouthwestern.edu
>> <histonet-request <@t> lists.utsouthwestern.edu
>> Message: 4
>> Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT)
>> From: kristen arvidson <arvidsonkristen <@t> yahoo.com>
>> Subject: [Histonet] Leica Paraplast
>> To: histonet <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID: <94596.93077.qm <@t> web65707.mail.ac4.yahoo.com>
>> Content-Type: text/plain; charset=iso-8859-1
>>
>> Has anyone who uses paraplast (we use the basic one) noticed a change in
>> the quality of your tissue? I have recently found out that they have
>> changed manufacturing sites in the past couple of months. I am having
>> on and off issues with my skin specimens that have been going on for
>> about 2 months or so. Thought there may be a correlation. Any thoughts?
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _________________________________________________________________
> Hotmail: Trusted email with Microsoft's powerful SPAM protection.
> http://clk.atdmt.com/GBL/go/210850552/direct/01/_________________________
> ______________________ Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------------------------------------
> CONFIDENTIALITY NOTICE:
> The information in this e-mail may be confidential and/or
> privileged. If you are not the intended recipient or an
> authorized representative of the intended recipient, you
> are hereby notified that any review, dissemination, or
> copying of this e-mail and its attachments, if any, or
> the information contained herein is prohibited. If you
> have received this e-mail in error, please immediately
> notify the sender by return e-mail and delete this e-mail
> from your computer system. Thank you.
> ============================================================
>
>
>
> ------------------------------
>
> Message: 17
> Date: Wed, 31 Mar 2010 05:35:49 -0700 (PDT)
> From: Ann Bennett <ann.bennettann <@t> yahoo.com>
> Subject: [Histonet] Thermo Fisher Excelsior Tissue Processor Programs
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <106398.85301.qm <@t> web114318.mail.gq1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> At one point we looked at getting an Excelsior and I spoke with our sales
> rep and he said all the reagents we use in our processor now are
> absolutely fine. He mentioned nothing about not being able to use
> Reagent alcohols or xylene substitutes. Hope this helps - have a happy
> histo day!
>
>
>
>
>
>
> ------------------------------
>
> Message: 18
> Date: Wed, 31 Mar 2010 08:42:06 -0400
> From: kdwyer3322 <@t> aol.com
> Subject: [Histonet] Texas Society for Histotechnology April 23-25,
> 2010
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <8CC9EE6B49D9799-17C0-9D76 <@t> webmail-m051.sysops.aol.com>
> Content-Type: text/plain; charset="us-ascii"
>
>
> Histonetters,
> It is not too late to join us for the 2010 TSH meeting in Houston Texas
> April 23-25, 2010.
>
> The fun starts Thursday April 22, 2010 with a Golf outing open to all
> Vendors and Attendees.
>
> Workshops begin Friday April 23, 2010 at 8:00am.
>
> There is still plenty of time to register and get a hotel room to enjoy 2
> full days of workshops and symposiums.
>
> If you would like a program go to txsh.org or contact me via this e-mail.
>
> Thanks,
> TSH Convention Committee
>
>
>
>
>
> ------------------------------
>
> Message: 19
> Date: Wed, 31 Mar 2010 10:13:25 -0400
> From: "Catherine Breen" <cmb321 <@t> excite.com>
> Subject: [Histonet] cassette labels erased by processor
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20100331101325.1573 <@t> web007.roc2.bluetie.com>
> Content-Type: text/plain; charset=UTF-8
>
> I am looking for help solving a lab mystery. The cassettes in our lab
> are labeled with an SP Securline Marker II and then processed in a Sakura
> VIP processor. Two weeks ago the entire batch came out labeled much more
> lightly than usual, some to the point of where the label was completely
> effaced. Our current theory is that an acid cleanser (Citronox) or
> possibly another acid was accidentally introduced into the processor. Has
> any lab experienced this problem before, especially with Citronox?
>
> Thank you.
>
> ------------------------------------------------------------
> Best Weight Loss Program - Click Here!
> Weight Loss Program
> http://tagline.excite.com/c?cp=etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZ
> mGMQTOAAYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY=
>
> ------------------------------
>
> Message: 20
> Date: Wed, 31 Mar 2010 10:28:42 -0400
> From: "Weems, Joyce" <JWeems <@t> sjha.org>
> Subject: [Histonet] RE: her2 validation
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <92AD9B20A6C38C4587A9FEBE3A30E16405E029D9 <@t> CHEXCMS10.one.ads.che.org>
> Content-Type: text/plain; charset="us-ascii"
>
>
>> From June-15, 2009 CAP checklist
>
>
>
> ANP.22997 Phase I N/A YES NO
>
> If the laboratory performs HER2 testing (HER2 protein over-expression by
> immunohistochemistry [IHC] or HER2 gene amplification by in situ
> hybridization [e.g. FISH, CISH*, SISH*, etc.]), has the laboratory
> documented appropriate validation for the assay(s)?
>
> NOTE: Initial test validation must be performed on a minimum of 25 cases
> (recommended 25-100). Validation may be performed by comparing the
> results of testing with a validated alternative method (i.e. IHC vs.
> FISH) either in the same laboratory or another laboratory, or with the
> same validated method performed in another laboratory; validation testing
> must be done using the same set of cases in both labs.
>
> If specimens are fixed in a medium other than 10% neutral buffered
> formalin, the validation study must show that results are concordant with
> results from formalin-fixed tissues.
>
> If significant changes are made in testing methods (e.g., antibody
> clone, antigen retrieval protocol or detection system, FISH probe or
> pretreatment protocol), revalidation is required.
>
> This checklist item applies to laboratories that perform the technical
> testing of specimens for HER2 amplification. Patient specimens should be
> fixed in the same manner as the specimens used for the validation
> study(ies).
>
> *CISH = chromogenic in-situ hybridization; SISH = silver-enhanced
> in-situ hybridization
>
>
> Joyce Weems
> Pathology Manager
> Saint Joseph's Hospital
> 5665 Peachtree Dunwoody Rd NE
> Atlanta, GA 30342
> 678-843-7376 - Phone
> 678-843-7831 - Fax
>
> Confidentiality Notice:
> This email, including any attachments is the
> property of Catholic Health East and is intended
> for the sole use of the intended recipient(s).
> It may contain information that is privileged and
> confidential. Any unauthorized review, use,
> disclosure, or distribution is prohibited. If you are
> not the intended recipient, please reply to the
> sender that you have received the message in
> error, then delete this message.
>
>
>
>
> ------------------------------
>
> Message: 21
> Date: Wed, 31 Mar 2010 10:33:35 -0400
> From: "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG>
> Subject: RE: [Histonet] cassette labels erased by processor
> To: "Catherine Breen" <cmb321 <@t> excite.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <073AE2BEA1C2BA4A8837AB6C4B943D9703E2414A <@t> PHSXMB30.partners.org>
> Content-Type: text/plain; charset="us-ascii"
>
> I had similar issues with all of the marking pens out there. We finally
> switched to Tissue-Tek Marking pencils #4160 and have not had a problem
> since. Plus they never "dry" out!
>
> Peggy
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Catherine
> Breen Sent: Wednesday, March 31, 2010 10:13 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] cassette labels erased by processor
>
> I am looking for help solving a lab mystery. The cassettes in our lab are
> labeled with an SP Securline Marker II and then processed in a Sakura VIP
> processor. Two weeks ago the entire batch came out labeled much more
> lightly than usual, some to the point of where the label was completely
> effaced. Our current theory is that an acid cleanser (Citronox) or
> possibly another acid was accidentally introduced into the processor.
> Has any lab experienced this problem before, especially with Citronox?
>
> Thank you.
>
> ------------------------------------------------------------
> Best Weight Loss Program - Click Here!
> Weight Loss Program
> http://tagline.excite.com/c?cp=etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZ
> mGMQTOA AYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY=
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> The information in this e-mail is intended only for the person to whom it
> is addressed. If you believe this e-mail was sent to you in error and the
> e-mail contains patient information, please contact the Partners
> Compliance HelpLine at http://www.partners.org/complianceline . If the
> e-mail was sent to you in error but does not contain patient information,
> please contact the sender and properly dispose of the e-mail.
>
>
>
>
> ------------------------------
>
> Message: 22
> Date: Wed, 31 Mar 2010 09:39:46 -0500
> From: Cheri Miller <cmiller <@t> physlab.com>
> Subject: FW: [Histonet] cassette labels erased by processor
> To: "histonet-bounces <@t> lists.utsouthwestern.edu"
> <histonet-bounces <@t> lists.utsouthwestern.edu>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <E3C81A010935EA41B379AC765103F3BF31B3728CE8 <@t> olsrv12>
> Content-Type: text/plain; charset="us-ascii"
>
>
>
> YES! That is why I use pencil only. I had a processor full of blank/
> unlabeled cassettes because the lot# of the pens was bad. There is no way
> of knowing if the ink is reagent proof until a disaster like you have
> occurs. You don't always know if the ink lot passed its QC with absolute
> certainty. Pencil is fail proof. I hope your day gets better, Cheri
>
> Cheryl Miller HT ASCP CM
> Histology Supervisor
> Physicians Laboratory Services
> Omaha, NE. 402 731 4148
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Catherine
> Breen Sent: Wednesday, March 31, 2010 9:13 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] cassette labels erased by processor
>
> I
>
> PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.
> If you are not the addressee intended / indicated or agent responsible
> for delivering it to the addressee, you are hereby notified that you are
> in possession of confidential and privileged information. Any
> dissemination, distribution, or copying of this e-mail is strictly
> prohibited. If you have received this message in error, please notify
> the sender immediately and delete this email from your system.
>
>
>
> ------------------------------
>
> Message: 23
> Date: Wed, 31 Mar 2010 15:40:44 +0100
> From: Malika Benatti <malbenatti <@t> googlemail.com>
> Subject: Re: [Histonet] cassette labels erased by processor
> To: Catherine Breen <cmb321 <@t> excite.com>
> Cc: Histonet List <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <F4BF17D2-D535-48B3-BC55-048524ECE07D <@t> gmail.com>
> Content-Type: text/plain; charset=us-ascii; format=flowed;
> delsp=yes
>
> Hi there,
>
> I would suggest to use a pencil rather than a marker pen to label
> cassettes when processing cassette with the Sakura VIP , my lab had a
> number of the so called solvent proof pen on trial and have yet to
> find one that survive processing.
>
>
>
>
> Malika Benatti BSc MIBMS
> Specialist Biomedical Scientist
> Great Ormond Street Children Hospital
> London
>
> " ... Smile it confuses people ..."
>
> On 31 Mar 2010, at 15:13, "Catherine Breen" <cmb321 <@t> excite.com> wrote:
>
>> I am looking for help solving a lab mystery. The cassettes in our
>> lab are labeled with an SP Securline Marker II and then processed in
>> a Sakura VIP processor. Two weeks ago the entire batch came out
>> labeled much more lightly than usual, some to the point of where the
>> label was completely effaced.
>> Our current theory is that an acid cleanser (Citronox) or possibly
>> another acid was accidentally introduced into the processor.
>> Has any lab experienced this problem before, especially with Citronox?
>>
>> Thank you.
>>
>> ------------------------------------------------------------
>> Best Weight Loss Program - Click Here!
>> Weight Loss Program
>> http://tagline.excite.com/c?
>> cp=
>> etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOAAYAAAAAAAAAAAAAAAAAAADN
>> AAAAAAAAAAAAAAAAAAAEUlAqCWY=
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 76, Issue 45
> ****************************************
>
>
>
> To the extent this electronic communication or any of its attachments
> contain information that is not in the public domain, such information is
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> _______________________________________________
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>
Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
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