[Histonet] RE: lab markers KP
Blazek, Linda
lblazek <@t> digestivespecialists.com
Wed Mar 31 10:31:28 CDT 2010
I have to agree. They have never failed and work forever.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Madary, Joseph
Sent: Wednesday, March 31, 2010 11:30 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] lab markers KP
The only consistent lab pen I have used that will hold up under decal, processing with various alcohol, xylene, hemo de is the KP marker plus. I am sure there are several vendors, I use Mercedes.
Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Mgr,
OMW, Area 4, Lab 2438
301.398.4745(vm)
301.398.6360(lab)
301.398.9745(fax)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, March 31, 2010 10:45 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 76, Issue 45
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Today's Topics:
1. Coverslipping with SubX (Vanessa Avalos)
2. Haunted cryostat/Thanks! (mtitford <@t> aol.com)
3. GMS on decal tissue (Morken, Tim)
4. questions (Webb, Dorothy L)
5. Re: her2 validation (Pat Laurie)
6. FREE MONEY! (Jackie M O'Connor)
7. RE: questions (Garrison, Becky)
8. Thermo's Excelsior Tissue Processor Program's
(Akemi Allison-Tacha)
9. re: cyto prep tech (Kim Tournear)
10. her-2 neu validation (Debbie Nannenga)
11. RE: questions (Tony Henwood)
12. RE: RE: Leica Paraplast (connie grubaugh)
13. (no subject) (Green JumpyOne)
14. Fwd: [Histonet] (no subject) (Malika Benatti)
15. RE: Thermo's Excelsior Tissue Processor Program's
(Heckford, Karen - SMMC-SF)
16. RE: RE: Leica Paraplast (Nails, Felton)
17. Thermo Fisher Excelsior Tissue Processor Programs (Ann Bennett)
18. Texas Society for Histotechnology April 23-25, 2010
(kdwyer3322 <@t> aol.com)
19. cassette labels erased by processor (Catherine Breen)
20. RE: her2 validation (Weems, Joyce)
21. RE: cassette labels erased by processor (Sherwood, Margaret )
22. FW: [Histonet] cassette labels erased by processor (Cheri Miller)
23. Re: cassette labels erased by processor (Malika Benatti)
----------------------------------------------------------------------
Message: 1
Date: Tue, 30 Mar 2010 11:15:38 -0700
From: "Vanessa Avalos" <vavalos <@t> allergydermatology.com>
Subject: [Histonet] Coverslipping with SubX
To: "'HISTONET LISTS'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001cad035$00d2f5b0$0278e110$@com>
Content-Type: text/plain; charset="us-ascii"
Is anyone using SubX ? I am trying it out and am having some difficulty
coverslipping. I am using the Subx glue as directed since Acrymount didn't
seem to work as well. I still get a hazy film under the glass and am getting
big air bubbles as well. I can eventually get them out but its just takes a
while and you know how time is precious when you have a line of slides to
stain. The process is not as smooth as before. I really would like this to
work out for me and eliminate xylene.
Any suggestions??
Vanessa
Fax: 602-277-2134
------------------------------
Message: 2
Date: Tue, 30 Mar 2010 14:44:26 -0400
From: mtitford <@t> aol.com
Subject: [Histonet] Haunted cryostat/Thanks!
To: Histonet <@t> pathology.swmed.edu
Message-ID: <8CC9E5028ADFB36-1500-2BE5 <@t> webmail-m010.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
Thank you everyone who responded to my problem with a Leica CM 1850 cryostat turning itself off. About 11 people responded with tips. Thank you!! The Histonet is great!!
In answer to some enquirys:
1) The cryostat is set to defrost at 2 a.m.. The manual says it defrosts for an hour. Jackie O' Conner asks if it is set to go back "on" after the defrost. I have no idea. It defrosts well the rest of the time, and turns itself back on. Brian Cornett-Early recommends changing the start device on the compressor.
2) Someone else asked if power is getting to the cryostat - Yes, when it turns itself off, the on/off switch is on "off". All you have to do is flip the switch and it starts pumping and cooling.
3) Mari Ann Mailhiot with Leica recommends changing a cicuit breaker on the side of the compressor. That is probably where we will start. Mari Ann works for Leica so it sounds like good advice.
Thank you everyone. Since it only breaks down about once a month, it will take a long time to determine if the problem is fixed.
Michael Titford
Pathology USA
Mobile AL USA
------------------------------
Message: 3
Date: Tue, 30 Mar 2010 11:52:17 -0700
From: "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: [Histonet] GMS on decal tissue
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1AAF670737F193429070841C6B2ADD4C013B16C780 <@t> EXMBMCB15.ucsfmedicalcenter.org>
Content-Type: text/plain; charset=us-ascii
Has anyone seen or heard of problems with Grocott Methenamine Silver staining for fungi on decal tissues? Specifically, background or false positives? I can't find anything in any books that gives any indications to not use decaled tissue.
Thanks!
Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
Box 1656
1600 Divisadero St.
San Francisco, CA 94143-1656
USA
Phone: (415) 514-6042
Pager: (415) 443-6509
Fax: (415) 885-7409
Email: tim.morken <@t> ucsfmedctr.org<mailto:tim.morken <@t> ucsfmedctr.org>
------------------------------
Message: 4
Date: Tue, 30 Mar 2010 14:03:55 -0500
From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] questions
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<65365F35C0F2EF4D846EC3CA73E49C43C5786C5846 <@t> HPEMX3.HealthPartners.int>
Content-Type: text/plain; charset="us-ascii"
We have been having problems with underprocessed placental membrane, some of which are cut fairly thick, but am seeing it on more than just the thich samples. Does anyone out there in histoland have a special process for placentas or any helpful hints? I do know that many times the placentas sit without formalin in L&D for hours before they bring them to histo and add formalin and this seems to me it could be a factor, even though we have them sitting in formalin for a few hours before processing.
Also, does anyone do the high-iron diamine stain for intestinal mucin staining? Do you do it with an Alcian Blue-PAS stain?
Thank you ahead of time for any and all responses!!
Dorothy Webb, HT (ASCP)
Regions Histology Lab
________________________________
This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.
If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0
------------------------------
Message: 5
Date: Tue, 30 Mar 2010 13:26:21 -0700
From: Pat Laurie <foreightl <@t> gmail.com>
Subject: Re: [Histonet] her2 validation
To: anita dudley <azdudley <@t> hotmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<bdfbc2371003301326n2031c2e2v2c0a2a193196c676 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
We left it up to our pathologist. Initially we did the 25 slide validation,
our pathologist wasn't 100% convinced that he could read them accurately, so
we did another 25, plus all of the ones we sent out which already had FISH
ran. It ended up being almost 70 cases, and our pathologist was properly
"educated" on how he should score them. He felt that there was excessive
pressure put on the pathologists due to having to score these slides, not
just say negative or positive. I'll say though for the last year or so,
with about 6 her2 cases ran a day, he has been remarkably accurate. Any
that he scores 2+ and has sent out for FISH he usually indicates if he
thinks that it will be positive or negative. He has yet to be wrong.
Another Pathologist who I talked to who helped set up the guidelines said
that there isn't a "1 method fits all" process. Good luck.
On Tue, Mar 30, 2010 at 7:19 AM, anita dudley <azdudley <@t> hotmail.com> wrote:
>
> sorry!!! I didn't put a subject in the previous post. wondering about how
> many slides people are using for her2 validation. thanks
>
>
>
> anita dudley
>
> providence hospital
>
> mobile alabama
>
> _________________________________________________________________
> The New Busy is not the old busy. Search, chat and e-mail from your inbox.
>
> http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3_______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
PH: 206-215-5949
plaurie <@t> cellnetix.com
------------------------------
Message: 6
Date: Tue, 30 Mar 2010 15:28:30 -0500
From: Jackie M O'Connor <Jackie.O'Connor <@t> abbott.com>
Subject: [Histonet] FREE MONEY!
To: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OFAB0D17A8.5145E414-ON862576F6.006FF8AE-862576F6.00708491 <@t> abbott.com>
Content-Type: text/plain; charset="US-ASCII"
Now that I have your attention - -
I would really like to hear from you if you are at all interested in a
histology position at Abbott Laboratories in Northern Illinois. Even if
you have applied previously.
This is a great opportunity to work in a GLP research lab, a good deal of
bench work, but developing IHC for toxicology purposes as well. We have
a Biocare intelliPATH stainer - it's FUN!
We have four full time technicians/technologists with a fifth open
position. Abbott offers 3 weeks paid vacation, great benefits, and is a
good company to work for. I've been here ten years, and I've never been
anywhere 10 years.
Please forward your resume to me, as well as go to www.Abbott.com to
apply.
Jackie O'Connor
------------------------------
Message: 7
Date: Tue, 30 Mar 2010 16:49:42 -0400
From: "Garrison, Becky" <becky.garrison <@t> jax.ufl.edu>
Subject: RE: [Histonet] questions
To: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B3CA1BAF6C5E4541867E4E79AD2412E006A5CFC0 <@t> jaxmail.umc.ufl.edu>
Content-Type: text/plain; charset="us-ascii"
We receive the placentas fresh (they are refrigerated in L&D before
transport to Pathology); add lots of formalin (use 163 oz containers)
and let fix overnight. Early next morning, placenta is grossed and
cassettes sit in formalin til end of day. This formalin is changed at
least once so that bloody formalin is replaced with fresh. Placed on
processor at end of second day after receipt.
When we had L&D add formalin, there was never enough formalin added and
the placentas sat unfixed at room temperature for long periods of time.
This procedure works better for us. Yes, we can not meet the CAP
guideline
for 2 day TAT but do end up with a consistently better quality product
for this tissue type. (Placentas make up a small portion of overall
workload, so overall TAT is not affected). Prior to this procedure,
placentas made
up a disproportionate amount of reprocessed blocks.
Becky Garrison
Pathology Supervisor
Shands Jacksonville
Jacksonville, FL 32209
904-244-6237, phone
904-244-4290, fax
904-393-3194, pager
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Webb,
Dorothy L
Sent: Tuesday, March 30, 2010 3:04 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] questions
We have been having problems with underprocessed placental membrane,
some of which are cut fairly thick, but am seeing it on more than just
the thich samples. Does anyone out there in histoland have a special
process for placentas or any helpful hints? I do know that many times
the placentas sit without formalin in L&D for hours before they bring
them to histo and add formalin and this seems to me it could be a
factor, even though we have them sitting in formalin for a few hours
before processing.
Also, does anyone do the high-iron diamine stain for intestinal mucin
staining? Do you do it with an Alcian Blue-PAS stain?
Thank you ahead of time for any and all responses!!
Dorothy Webb, HT (ASCP)
Regions Histology Lab
________________________________
This e-mail and any files transmitted with it are confidential and are
intended solely for the use of the individual or entity to whom they are
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responsible for delivering the e-mail to the intended recipient, please
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strictly prohibited.
If you have received this e-mail in error, please immediately notify the
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be reimbursed for reasonable costs incurred in notifying us.
HealthPartners R001.0
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Tue, 30 Mar 2010 16:04:19 -0700 (PDT)
From: Akemi Allison-Tacha <akemiat3377 <@t> yahoo.com>
Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's
To: histo net <histonet <@t> pathology.swmed.edu>
Message-ID: <465571.85431.qm <@t> web113812.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi All of you in Histoland,
I am working with a facility that recently purchased a Thermo Excelsior Tissue Processor. They have had processing problems with several of their specimens using non-xylene clearing agent. These issues were with both bx's and larger tissues. The program and the non-xylene clearing agent was recommended by the technical staff at Thermo.
The histology staff have switched back to using xylene verses the non-xylene clearing agent, and most of the issues have disappeared.
Also, the histotech's were using reagent alcohol, histological grade. This grade of alcohol is denatured with methyl alcohol. The sales representative was in and informed us that this type of alcohol has damaging effects on the instrument. The representative will be coming in to flush out the system and reprogram the instrument next Monday.
I looked over the current VIP program which is being used, and the program, timing and temperatures are a little different from most hospital and private laboratories I have worked with. We are going to use basically the same program for the Excelsior, that we use on the VIP.
I would like to know what other Excelsior Tissue Processor users that use xylene have for their programs. It would be great to compare the reagents, programs, timing, and temperatures for Routine overnight runs and for Rapid Biopsy Runs. Thank you in advance for your assistance.
Akemi Allison-Tacha BS, HT(ASCP)HTL
Director
Phoenix Lab Consulting
E-Mail: akemiat3377 <@t> yahoo.com
------------------------------
Message: 9
Date: Tue, 30 Mar 2010 16:05:40 -0700 (PDT)
From: Kim Tournear <kimtournear <@t> yahoo.com>
Subject: [Histonet] re: cyto prep tech
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <839809.46245.qm <@t> web54204.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Thank you to everyone who responded to my question about cyto prep tech work,
~Kim Tournear ~ HT (ASCP), QIHC (ASCP)
Histology Supervisor
Tucson Medical Center
Tucson, AZ
~Don't let your life end before it begins~
OU Rocks!!!!
------------------------------
Message: 10
Date: Tue, 30 Mar 2010 17:12:57 -0700
From: Debbie Nannenga <histowa13 <@t> hotmail.com>
Subject: [Histonet] her-2 neu validation
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT118-W22EF34188ABEA72E7468D3B21E0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Hi Anita,
We ran 25 amplified and 25 negative cases for our validation study.
Good Luck.
Debbie Nannenga, HTL(ASCP) QIHC
InCyte Pathology
Spokane, WA
------------------------------
Message: 11
Date: Wed, 31 Mar 2010 11:21:58 +1100
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] questions
To: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC555206853921 <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"
Dorothy,
Apart from hoping the blocks of tissue are not too thick, we microwave
the cassettes in 10%NBF in a Milestone Mega TT - 2 hours at 45oC. This
ensures adequate fixation prior to processing on a Shandon Excelsior
Tissue Processor.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Webb,
Dorothy L
Sent: Wednesday, 31 March 2010 6:04 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] questions
We have been having problems with underprocessed placental membrane,
some of which are cut fairly thick, but am seeing it on more than just
the thich samples. Does anyone out there in histoland have a special
process for placentas or any helpful hints? I do know that many times
the placentas sit without formalin in L&D for hours before they bring
them to histo and add formalin and this seems to me it could be a
factor, even though we have them sitting in formalin for a few hours
before processing.
Also, does anyone do the high-iron diamine stain for intestinal mucin
staining? Do you do it with an Alcian Blue-PAS stain?
Thank you ahead of time for any and all responses!!
Dorothy Webb, HT (ASCP)
Regions Histology Lab
________________________________
This e-mail and any files transmitted with it are confidential and are
intended solely for the use of the individual or entity to whom they are
addressed. If you are not the intended recipient or the individual
responsible for delivering the e-mail to the intended recipient, please
be advised that you have received this e-mail in error and that any use,
dissemination, forwarding, printing, or copying of this e-mail is
strictly prohibited.
If you have received this e-mail in error, please immediately notify the
HealthPartners Support Center by telephone at (952) 967-6600. You will
be reimbursed for reasonable costs incurred in notifying us.
HealthPartners R001.0 _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
*********************************************************************************
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Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead
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------------------------------
Message: 12
Date: Tue, 30 Mar 2010 19:58:21 -0700
From: connie grubaugh <conniegrubaugh <@t> hotmail.com>
Subject: RE: [Histonet] RE: Leica Paraplast
To: <ddreesen <@t> sbcglobal.net>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT104-W197006F3C487BD2D0A97E9D61E0 <@t> phx.gbl>
Content-Type: text/plain; charset="Windows-1252"
Hi all, I questioned the Leica paraplast that we have received too. It is real gummy and sticky. Takes me forever to cut. I asked and was informed that it is the same stuff we have always got and there is no difference. Except all of us techs have noticed a big difference.
Connie G.
> Date: Tue, 23 Mar 2010 11:29:03 -0700
> From: ddreesen <@t> sbcglobal.net
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] RE: Leica Paraplast
>
>
> Hi Kristen,
> We were using the Paraplast Xtra and switched to something else after we noticed a difference in the quality of the paraffin. The tissues weren't cutting as well and the paraffin seemed to be "gritty". We found we were going through many more blades due to nicks and scratches and many times had to switch blades mid-block.
>
>
> From: histonet-request <@t> lists.utsouthwestern.edu <histonet-request <@t> lists.utsouthwestern.edu
> Message: 4
> Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT)
> From: kristen arvidson <arvidsonkristen <@t> yahoo.com>
> Subject: [Histonet] Leica Paraplast
> To: histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <94596.93077.qm <@t> web65707.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Has anyone who uses paraplast (we use the basic one) noticed a change in the quality of your tissue? I have recently found out that they have changed manufacturing sites in the past couple of months. I am having on and off issues with my skin specimens that have been going on for about 2 months or so. Thought there may be a correlation. Any thoughts?
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_________________________________________________________________
Hotmail: Trusted email with Microsoft's powerful SPAM protection.
http://clk.atdmt.com/GBL/go/210850552/direct/01/
------------------------------
Message: 13
Date: Wed, 31 Mar 2010 01:52:42 -0700
From: Green JumpyOne <greenjumpyone <@t> hotmail.com>
Subject: [Histonet] (no subject)
To: <aga8ton <@t> live.com>, <histonet <@t> lists.utsouthwestern.edu>,
<jkbrown2986 <@t> hotmail.com>
Message-ID: <BLU128-W26777912678997BEEAAEB4AB1E0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
http://www.trainedlabor.com/H6UH4Yh1UJ.html
_________________________________________________________________
The New Busy is not the old busy. Search, chat and e-mail from your inbox.
http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3
------------------------------
Message: 14
Date: Wed, 31 Mar 2010 10:45:09 +0100
From: Malika Benatti <malbenatti <@t> googlemail.com>
Subject: Fwd: [Histonet] (no subject)
To: Histonet List <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <8186A0A0-94D8-4E31-99D1-87B35FC3CDC6 <@t> gmail.com>
Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes
Dear Histonet list manager
Is they anyway you could filter/block spam such as this one from be
sent out to the Histonet list.
Cheers
Malika Benatti BSc MIBMS
Specialist Biomedical Scientist
Great Ormond Street Children Hospital
London
" ... Smile it confuses people ..."
Begin forwarded message:
> From: Green JumpyOne <greenjumpyone <@t> hotmail.com>
> Date: 31 March 2010 09:52:42 GMT+01:00
> To: <aga8ton <@t> live.com>, <histonet <@t> lists.utsouthwestern.edu>, <jkbrown2986 <@t> hotmail.com
> >
> Subject: [Histonet] (no subject)
>
> http://www.trainedlabor.com/H6UH4Yh1UJ.html
>
> _________________________________________________________________
> The New Busy is not the old busy. Search, chat and e-mail from your
> inbox.
> http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL:en-US:WM_HMP:032010_3_______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Wed, 31 Mar 2010 05:02:26 -0700
From: "Heckford, Karen - SMMC-SF" <Karen.Heckford <@t> CHW.edu>
Subject: RE: [Histonet] Thermo's Excelsior Tissue Processor Program's
To: "Akemi Allison-Tacha" <akemiat3377 <@t> yahoo.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<2842DC75AE43AA4B92954CFB31781BC105697F76 <@t> CHW-MSG-301.chw.edu>
Content-Type: text/plain; charset="iso-8859-1"
Pretty much any problems I have had with the Excelsior and I have been using one for 4 years now is with Pathologists loading it incorrectly. I use reagent alcohol in it all the time, with no problems. I prefer using xylene in my processor. Every single time I have switched and started using a non-xylene sub. I have had nothing but problems. If I use a non-xylene I save it for the stainer.
Take it easy,
Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha
Sent: Tuesday, March 30, 2010 4:04 PM
To: histo net
Subject: [Histonet] Thermo's Excelsior Tissue Processor Program's
Hi All of you in Histoland,
I am working with a facility that recently purchased a Thermo Excelsior Tissue Processor. They have had processing problems with several of their specimens using non-xylene clearing agent. These issues were with both bx's and larger tissues. The program and the non-xylene clearing agent was recommended by the technical staff at Thermo.
The histology staff have switched back to using xylene verses the non-xylene clearing agent, and most of the issues have disappeared.
Also, the histotech's were using reagent alcohol, histological grade. This grade of alcohol is denatured with methyl alcohol. The sales representative was in and informed us that this type of alcohol has damaging effects on the instrument. The representative will be coming in to flush out the system and reprogram the instrument next Monday.
I looked over the current VIP program which is being used, and the program, timing and temperatures are a little different from most hospital and private laboratories I have worked with. We are going to use basically the same program for the Excelsior, that we use on the VIP.
I would like to know what other Excelsior Tissue Processor users that use xylene have for their programs. It would be great to compare the reagents, programs, timing, and temperatures for Routine overnight runs and for Rapid Biopsy Runs. Thank you in advance for your assistance.
Akemi Allison-Tacha BS, HT(ASCP)HTL
Director
Phoenix Lab Consulting
E-Mail: akemiat3377 <@t> yahoo.com
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Message: 16
Date: Wed, 31 Mar 2010 07:27:34 -0500
From: "Nails, Felton" <flnails <@t> texaschildrens.org>
Subject: RE: [Histonet] RE: Leica Paraplast
To: "'connie grubaugh'" <conniegrubaugh <@t> hotmail.com>,
"ddreesen <@t> sbcglobal.net" <ddreesen <@t> sbcglobal.net>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C1FE5960057C084CA389CE97779062904C52DEFE <@t> TCDMSG01.ad.TexasChildrensHospital.org>
Content-Type: text/plain; charset=us-ascii
There is about three brands of paraplast and they all cut differently. In one of my labs I use paraplast plus and it ribbons very well however the blocks have to be colder then if you are using TissuePrep from Fisher.
Leica may have sent you one of the other types of paraplast. (paraplast, Paraplast Plus, Paraplast Xtra)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of connie grubaugh
Sent: Tuesday, March 30, 2010 9:58 PM
To: ddreesen <@t> sbcglobal.net; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Leica Paraplast
Hi all, I questioned the Leica paraplast that we have received too. It is real gummy and sticky. Takes me forever to cut. I asked and was informed that it is the same stuff we have always got and there is no difference. Except all of us techs have noticed a big difference.
Connie G.
> Date: Tue, 23 Mar 2010 11:29:03 -0700
> From: ddreesen <@t> sbcglobal.net
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] RE: Leica Paraplast
>
>
> Hi Kristen,
> We were using the Paraplast Xtra and switched to something else after we noticed a difference in the quality of the paraffin. The tissues weren't cutting as well and the paraffin seemed to be "gritty". We found we were going through many more blades due to nicks and scratches and many times had to switch blades mid-block.
>
>
> From: histonet-request <@t> lists.utsouthwestern.edu
> <histonet-request <@t> lists.utsouthwestern.edu
> Message: 4
> Date: Tue, 23 Mar 2010 08:12:18 -0700 (PDT)
> From: kristen arvidson <arvidsonkristen <@t> yahoo.com>
> Subject: [Histonet] Leica Paraplast
> To: histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <94596.93077.qm <@t> web65707.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Has anyone who uses paraplast (we use the basic one) noticed a change in the quality of your tissue? I have recently found out that they have changed manufacturing sites in the past couple of months. I am having on and off issues with my skin specimens that have been going on for about 2 months or so. Thought there may be a correlation. Any thoughts?
> _______________________________________________
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 17
Date: Wed, 31 Mar 2010 05:35:49 -0700 (PDT)
From: Ann Bennett <ann.bennettann <@t> yahoo.com>
Subject: [Histonet] Thermo Fisher Excelsior Tissue Processor Programs
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <106398.85301.qm <@t> web114318.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
At one point we looked at getting an Excelsior and I spoke with our sales rep and he said all the reagents we use in our processor now are absolutely fine. He mentioned nothing about not being able to use Reagent alcohols or xylene substitutes. Hope this helps - have a happy histo day!
------------------------------
Message: 18
Date: Wed, 31 Mar 2010 08:42:06 -0400
From: kdwyer3322 <@t> aol.com
Subject: [Histonet] Texas Society for Histotechnology April 23-25,
2010
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CC9EE6B49D9799-17C0-9D76 <@t> webmail-m051.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
Histonetters,
It is not too late to join us for the 2010 TSH meeting in Houston Texas April 23-25, 2010.
The fun starts Thursday April 22, 2010 with a Golf outing open to all Vendors and Attendees.
Workshops begin Friday April 23, 2010 at 8:00am.
There is still plenty of time to register and get a hotel room to enjoy 2 full days of workshops and symposiums.
If you would like a program go to txsh.org or contact me via this e-mail.
Thanks,
TSH Convention Committee
------------------------------
Message: 19
Date: Wed, 31 Mar 2010 10:13:25 -0400
From: "Catherine Breen" <cmb321 <@t> excite.com>
Subject: [Histonet] cassette labels erased by processor
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20100331101325.1573 <@t> web007.roc2.bluetie.com>
Content-Type: text/plain; charset=UTF-8
I am looking for help solving a lab mystery. The cassettes in our lab are labeled with an SP Securline Marker II and then processed in a Sakura VIP processor. Two weeks ago the entire batch came out labeled much more lightly than usual, some to the point of where the label was completely effaced.
Our current theory is that an acid cleanser (Citronox) or possibly another acid was accidentally introduced into the processor.
Has any lab experienced this problem before, especially with Citronox?
Thank you.
------------------------------------------------------------
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------------------------------
Message: 20
Date: Wed, 31 Mar 2010 10:28:42 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: [Histonet] RE: her2 validation
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<92AD9B20A6C38C4587A9FEBE3A30E16405E029D9 <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"
>From June-15, 2009 CAP checklist
ANP.22997 Phase I N/A YES NO
If the laboratory performs HER2 testing (HER2 protein over-expression by immunohistochemistry [IHC] or HER2 gene amplification by in situ hybridization [e.g. FISH, CISH*, SISH*, etc.]), has the laboratory documented appropriate validation for the assay(s)?
NOTE: Initial test validation must be performed on a minimum of 25 cases (recommended 25-100). Validation may be performed by comparing the results of testing with a validated alternative method (i.e. IHC vs. FISH) either in the same laboratory or another laboratory, or with the same validated method performed in another laboratory; validation testing must be done using the same set of cases in both labs.
If specimens are fixed in a medium other than 10% neutral buffered formalin, the validation study must show that results are concordant with results from formalin-fixed tissues.
If significant changes are made in testing methods (e.g., antibody clone, antigen retrieval protocol or detection system, FISH probe or pretreatment protocol), revalidation is required.
This checklist item applies to laboratories that perform the technical testing of specimens for HER2 amplification. Patient specimens should be fixed in the same manner as the specimens used for the validation study(ies).
*CISH = chromogenic in-situ hybridization; SISH = silver-enhanced in-situ hybridization
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
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Message: 21
Date: Wed, 31 Mar 2010 10:33:35 -0400
From: "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG>
Subject: RE: [Histonet] cassette labels erased by processor
To: "Catherine Breen" <cmb321 <@t> excite.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<073AE2BEA1C2BA4A8837AB6C4B943D9703E2414A <@t> PHSXMB30.partners.org>
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I had similar issues with all of the marking pens out there. We finally
switched to Tissue-Tek Marking pencils #4160 and have not had a problem since.
Plus they never "dry" out!
Peggy
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Catherine Breen
Sent: Wednesday, March 31, 2010 10:13 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cassette labels erased by processor
I am looking for help solving a lab mystery. The cassettes in our lab are
labeled with an SP Securline Marker II and then processed in a Sakura VIP
processor. Two weeks ago the entire batch came out labeled much more lightly
than usual, some to the point of where the label was completely effaced.
Our current theory is that an acid cleanser (Citronox) or possibly another acid
was accidentally introduced into the processor.
Has any lab experienced this problem before, especially with Citronox?
Thank you.
------------------------------------------------------------
Best Weight Loss Program - Click Here!
Weight Loss Program
http://tagline.excite.com/c?cp=etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOA
AYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY=
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Message: 22
Date: Wed, 31 Mar 2010 09:39:46 -0500
From: Cheri Miller <cmiller <@t> physlab.com>
Subject: FW: [Histonet] cassette labels erased by processor
To: "histonet-bounces <@t> lists.utsouthwestern.edu"
<histonet-bounces <@t> lists.utsouthwestern.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <E3C81A010935EA41B379AC765103F3BF31B3728CE8 <@t> olsrv12>
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YES! That is why I use pencil only. I had a processor full of blank/ unlabeled cassettes because the lot# of the pens was bad. There is no way of knowing if the ink is reagent proof until a disaster like you have occurs. You don't always know if the ink lot passed its QC with absolute certainty. Pencil is fail proof. I hope your day gets better, Cheri
Cheryl Miller HT ASCP CM
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Catherine Breen
Sent: Wednesday, March 31, 2010 9:13 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cassette labels erased by processor
I
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Message: 23
Date: Wed, 31 Mar 2010 15:40:44 +0100
From: Malika Benatti <malbenatti <@t> googlemail.com>
Subject: Re: [Histonet] cassette labels erased by processor
To: Catherine Breen <cmb321 <@t> excite.com>
Cc: Histonet List <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <F4BF17D2-D535-48B3-BC55-048524ECE07D <@t> gmail.com>
Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes
Hi there,
I would suggest to use a pencil rather than a marker pen to label
cassettes when processing cassette with the Sakura VIP , my lab had a
number of the so called solvent proof pen on trial and have yet to
find one that survive processing.
Malika Benatti BSc MIBMS
Specialist Biomedical Scientist
Great Ormond Street Children Hospital
London
" ... Smile it confuses people ..."
On 31 Mar 2010, at 15:13, "Catherine Breen" <cmb321 <@t> excite.com> wrote:
> I am looking for help solving a lab mystery. The cassettes in our
> lab are labeled with an SP Securline Marker II and then processed in
> a Sakura VIP processor. Two weeks ago the entire batch came out
> labeled much more lightly than usual, some to the point of where the
> label was completely effaced.
> Our current theory is that an acid cleanser (Citronox) or possibly
> another acid was accidentally introduced into the processor.
> Has any lab experienced this problem before, especially with Citronox?
>
> Thank you.
>
> ------------------------------------------------------------
> Best Weight Loss Program - Click Here!
> Weight Loss Program
> http://tagline.excite.com/c?
> cp=
> etyBHPAubWg3PJMURG67IAAAKZQW4ZcSHBdS1PQuiZmGMQTOAAYAAAAAAAAAAAAAAAAAAADNAAAAAAAAAAAAAAAAAAAEUlAqCWY=
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End of Histonet Digest, Vol 76, Issue 45
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