[Histonet] Re: Histonet Digest, Vol 76, Issue 38

Thu Mar 25 13:31:51 CDT 2010



On 3/25/10 1:04 PM, "histonet-request <@t> lists.utsouthwestern.edu"
<histonet-request <@t> lists.utsouthwestern.edu> wrote:

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> Today's Topics:
> 
>    1. Re: H & E QC (Sue)
>    2. RE: H & E QC (WILLIAM DESALVO)
>    3. Re: number of slides (Anne van Binsbergen)
>    4. LOOKING FOR IHC POSITION (Isaac O)
>    5. Re: H & E QC (Malika Benatti)
>    6. RE: LOOKING FOR IHC POSITION (Plewinski, Amy)
>    7. Symphony (Kelly Boyd)
>    8. Job Opportunity (Jackie M O'Connor)
>    9. A.O TP8000 Processor (Cheryl Crowder)
>   10. RE: Symphony (CHRISTIE GOWAN)
>   11. Re: Silver Lips and Fingers (Andrea Grantham)
>   12. Troubleshooting IHC (Adam .)
>   13. Benchmark Ultra (Troutman, Kenneth A)
>   14. coverslipper (Margaryan, Naira)
>   15. RE: coverslipper (Rathborne, Toni)
>   16. Re: chick embryo frozen sections (Atoska Gentry)
>   17. Re: Acetone Fixation (Atoska Gentry)
>   18. RE: coverslipper (Nails, Felton)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Wed, 24 Mar 2010 23:02:35 +0000 (UTC)
> From: Sue <suetp918 <@t> comcast.net>
> Subject: Re: [Histonet] H & E QC
> To: ADESUPO ADESUYI <adesupo2002 <@t> hotmail.com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <1021401464.930521269471755920.JavaMail.root <@t> sz0028a.westchester.pa.mail.comca
> st.net>
> 
> Content-Type: text/plain; charset=utf-8
> 
> We have automatic stainers, so after the stainer is set up a slide with a
> micro-array is run. This slide is
> checked by the histologists and logged in. The next slides run are our rapid
> cases. A log sheet is
> prepared and handed to the pathologist with the slides and they are graded for
> processing, embedding, microtomy
> and staining. This log is turned into the supervisor and reviewed daily. If
> there are issues the supervisor will
> review with the histologists. Since the techs rotate weekly we are able to
> monitor all the tech's technical performance.
> 
> Susan T. Paturzo 
> TJUH 
> 
> 
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Wed, 24 Mar 2010 21:22:34 -0600
> From: WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>
> Subject: RE: [Histonet] H & E QC
> To: <adesupo2002 <@t> hotmail.com>, histonet
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BLU103-W2204B7C6BA7D8C137DF3F491240 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
> 
> 
> Whether you are using an automated stainer or hand staining, run a control
> slide and review before any patient samples are stained. I also suggest that
> only start or endpoint QC is not enough and you should consider incorporating
> continuous QC/QA at regular intervals for the stain set-up, to ensure the
> highest quality and provide adequate control of the process. You should be
> able to determine, in a very short time, the end point of the stain set up and
> then add QC checks for slide quality at 1/3 and 2/3 through the run or anytime
> a solution container is changed or rotated.
> 
>  
> 
> In our lab, with the regents used and staining protocols available to select,
> we have determined that a stain set of solutions, on our automated instrument,
> will maintain agreed and desired quality the pathologist will accept for 1500
> slides (I strongly suggest counting slides, not runs or racks). We stop
> processing patient slides and run the control slide at runs 1, 500, 1000 (+-
> 10% to allow for process flow and variance). The slides are reviewed for
> acceptance or rejection by a Coordinator or higher and when acceptable,patient
> slides may be placed on the instrument. All QC slides are saved for review and
> the QC maintenance sheet is filed daily. This process captures the employee
> that set up and monitors the instrument and the employee that QC'd along w/
> the QC review results. The control slide is a multi-tissue slide that must
> contain the four highest volume tissue types for the lab. Each pathologist
> receives a daily Quality Review sheet to report any variance, issues or
> problems for all cases read.
> 
>  
> 
> Think through your process, communicate with the pathologist and develop a
> QC/QA system/process that creates accountability for all employees, supports
> production of quality results and meets your regulatory needs.
> 
> William DeSalvo, B.S., HTL(ASCP)
> System Production Manager
> 
> Sonora Quest Laboratories
> 
> NSH Quality Control Committee Chairperson
> 
>  
>> From: adesupo2002 <@t> hotmail.com
>> To: histonet <@t> lists.utsouthwestern.edu
>> Date: Wed, 24 Mar 2010 17:53:12 -0400
>> Subject: [Histonet] H & E QC
>>>  
>> Hi,
>> 
>> I will appreciate it, if you guys could share your method/procedure for H & E
>> QC with me. Thanking you all for your usual cooperation.
>> 
>> 
>> 
>> Adesupo A.
>> 
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> ------------------------------
> 
> Message: 3
> Date: Thu, 25 Mar 2010 13:34:12 +0400
> From: Anne van Binsbergen <annigyg <@t> gmail.com>
> Subject: Re: [Histonet] number of slides
> To: hymclab <hymclab.hymclab <@t> ministryhealth.org>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <f8332fbe1003250234o100dadf4pe616d87dfbfc0959 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
> 
> Here is our basic microtomy protocol:
> 
> BMT - 3 H&Es, each has a ribbon, plus PAS, Retic, Perls
> Liver bx - 3 H&Es, each has a ribbon plus Retic, Trichrome, Perls,
> Renal bx - 4 H&Es, each has a short ribbon plus PAS, PMS, trichrome - on
> slides with gloms
> Breast bx - 3 H&Es, each has a ribbon
> Derm bx - 4 H&Es, each has a ribbon, one Path likes AP PAS on all punch bx's
> GIT bx - 3 H&Es, each has a ribbon, plus HP
> 
> all other small bx's get 3 H&Es, each with a ribbon
> 
> and then we wait for the orders for levels and deepers and..and...and
> 
> AbuDhabiAnnie
> 
> 
> 
> On 25 March 2010 00:19, hymclab <hymclab.hymclab <@t> ministryhealth.org> wrote:
> 
>> We follow the same practice as Susan.  Our Pathologists would rather have
>> more than less!!!
>> 
>> Dawn
>> 
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
>> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sue
>> Sent: Tuesday, March 23, 2010 5:08 PM
>> To: anita dudley
>> Cc: histonet <@t> lists.utsouthwestern.edu
>> Subject: Re: [Histonet] number of slides
>> 
>> We do three levels on all diagnostic biopsies. Liver and Kidney also get
>> upfront special stains, as do gastric biopsies (h pylori) We did cut down on
>> extra unstained slides since we were discarding most of them. As far as
>> stopping levels, my pathologist thinks it goes against standard of care. As
>> for prostate biopsies, they are so small any more, that we are thinking o
>> cutting 4 slides staining 1and 4 for H&E and holding 2 and 3 for possible
>> IHC. We are finding that when we have to go back there is minimal tumor for
>> IHC demonstration.
>> 
>> Susan T. Paturzo
>> Thomas Jefferson University Hospital
>> 
>> 
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