[Histonet] H & E QC
malbenatti <@t> googlemail.com
Thu Mar 25 06:15:58 CDT 2010
HE QC should be carry out on every slides that are stained, if you are checking for the staining intensity, this will be carried out using a control slide on new batch of staining solution, commercial or house made. Batch number should be recorded, and slides labelled with batch reference, date and file accordingly, the same goes for daily QC of routine work. Prior each morning run a control slide is run QC.
HE QC goes beyond staining intensity, as a number of artefacts can be introduced and HE unacceptable ( Shatters, creases, folds, scores, sections cut too thick, scams ... to name fews ) and therefore each slides stained should be QC by experience Staff prior been sent out to pathologist, this QC should be recorded and audited on a regular basis.
Furthermore over here in the UK every laboratory belong to the UK-NEQAS http://www.ukneqas.org.uk/ and external body that run a number of EQA Schemes for histopathology.
For HE EQA for each run the NEQAS will ask to select slides a number of HE slides that were produce at a randomly selected date. Slide will then be send externally and score by external accessor, and benchmark against all the participant in the scheme. If more score too low, lab will be flag and investigated.
For those of you in the US, I would be curious to know if you have a similar system, and how they work.
On 25 Mar 2010, at 03:22, WILLIAM DESALVO wrote:
> Whether you are using an automated stainer or hand staining, run a control slide and review before any patient samples are stained. I also suggest that only start or endpoint QC is not enough and you should consider incorporating continuous QC/QA at regular intervals for the stain set-up, to ensure the highest quality and provide adequate control of the process. You should be able to determine, in a very short time, the end point of the stain set up and then add QC checks for slide quality at 1/3 and 2/3 through the run or anytime a solution container is changed or rotated.
> In our lab, with the regents used and staining protocols available to select, we have determined that a stain set of solutions, on our automated instrument, will maintain agreed and desired quality the pathologist will accept for 1500 slides (I strongly suggest counting slides, not runs or racks). We stop processing patient slides and run the control slide at runs 1, 500, 1000 (+- 10% to allow for process flow and variance). The slides are reviewed for acceptance or rejection by a Coordinator or higher and when acceptable,patient slides may be placed on the instrument. All QC slides are saved for review and the QC maintenance sheet is filed daily. This process captures the employee that set up and monitors the instrument and the employee that QC'd along w/ the QC review results. The control slide is a multi-tissue slide that must contain the four highest volume tissue types for the lab. Each pathologist receives a daily Quality Review sheet to report any variance, issues or problems for all cases read.
> Think through your process, communicate with the pathologist and develop a QC/QA system/process that creates accountability for all employees, supports production of quality results and meets your regulatory needs.
> William DeSalvo, B.S., HTL(ASCP)
> System Production Manager
> Sonora Quest Laboratories
> NSH Quality Control Committee Chairperson
>> From: adesupo2002 <@t> hotmail.com
>> To: histonet <@t> lists.utsouthwestern.edu
>> Date: Wed, 24 Mar 2010 17:53:12 -0400
>> Subject: [Histonet] H & E QC
>> I will appreciate it, if you guys could share your method/procedure for H & E QC with me. Thanking you all for your usual cooperation.
>> Adesupo A.
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