[Histonet] Cover glass

Marcia Funk FUNKM <@t> mercyhealth.com
Wed Mar 24 16:02:57 CDT 2010

We run 700 to 800 slides per day plus specials and IHC's.  We use to wipe down
but with staff and workload it was impossible to continue.  Thanks for the input
and the remember.
Marcia Funk 
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401

>>> Merced M Leiker <leiker <@t> buffalo.edu> 03/24/2010 3:33 PM >>>
I use VWR VistaVision coverslips (for fluorescent imaging) and had noticed 
some dust/debris on them that would autofluoresce and give false positives 
with some of my stains.  The problem disappeared when I got into the habit 
wiping all my coverslips with EtOH before mounting...this may be cumbersome 
if you're doing a lot of slides, however. But it has worked really well.


--On Wednesday, March 24, 2010 4:24 PM -0400 Helen Fedor <hfedor <@t> jhmi.edu> 

> Hello, Our lab does IHC staining in house and have had zero problems with
> our Fisher brand cover slips until recently. We've tried both Fisher and
> Corning cover slips and what appears to be dust or imperfections in the
> glass are found on both brands. This is creating false positives for us,
> because when the positive staining is low it is difficult to impossible
> to tell the imperfections from the staining itself.
> Any advice will be welcome.
> --
> Helen
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu 
716-829-6118 (Ph)
716-829-2665 (Fx)

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