[Histonet] number of slides

[Malika Benatti]

Routinely

Gastric bx  upper / rt colon / lt colon one rubbon of 4 sections per slides
Liver HE @ level 1/2/3 and liver specials, AE1 IHC
Renal Biopsy (Native and Transplant) 24 slides inc HE/Renal Special/ IHC and
USS
TransBronchial BX HE @ level 1/2/3 and lung special
BMT HE, retic and IHC for NBLX case
Endomyocardial BX, HE @level 1/2/3 EVG/MT and C4d IHC
Vascular Malformation HE and IHC
Surgical Heart (11 blocks) HE, EVG on all Blocks
Cornea Button HE, PAS, AB, Congo red
All Tumour BX HE, and 10 USS for IHC
PM Brain HE/LFB on all blocks
PM Heart HE/EVG on all blocks
PM Lung HE/EVG/PERLS on all blocks

Working in paediatric I have not seen a prostate of a breast for the past 6
years, but my old lab had between 4 and 6 blocks per prostate or breast
tissue and were cutting HE at level 1/2/3 and picking up 4 USS between level
for further IHC.


Malika

Malika Benatti
Specialist Biomedical Scientist
Great Ormond Street Hospital for Children


On Tue, Mar 23, 2010 at 10:08 PM, Sue <suetp918 <@t> comcast.net> wrote:

> We do three levels on all diagnostic biopsies. Liver and Kidney also get
> upfront special stains, as do gastric biopsies (h pylori)
> We did cut down on extra unstained slides since we were discarding most of
> them. As far as stopping levels, my pathologist thinks it goes against
> standard
> of care. As for prostate biopsies, they are so small any more, that we are
> thinking o cutting 4 slides staining 1and 4 for H&E and holding 2 and 3 for
> possible IHC. We
> are finding that when we have to go back there is minimal tumor for IHC
> demonstration.
>
> Susan T. Paturzo
> Thomas Jefferson University Hospital
>
>
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