[Histonet] mouse perfusion rate

[Merced M Leiker]

That may be mouse cardiac output, but I can assure you, from experience, 
you do not want to perfuse at 17ml/min.

Regards,
Merced

--On Thursday, March 18, 2010 1:32 PM -0400 MKing <making <@t> ufl.edu> wrote:

> Li,
>
> Mouse cardiac output seems to be about 17 ml/min (e.g.
> www.transonic.com/mice1.shtml), you probably want to try for that to keep
> pressures close to physiological.
> A syringe pump is pretty inexpensive and probably all you need.
>
> Mike
>
> ----- Original Message -----
> From: Li Zhang <dancingwing <@t> yahoo.com>
> Date: Wednesday, March 17, 2010 14:59
> Subject: [Histonet] question about mouse perfusion
> To: histonet <@t> lists.utsouthwestern.edu
>
>  > > My question is: can anyone give me a rough idea of how fast I
>  > > should inject ( like ml/min). I think I've tried like 30 ml in 3
>  > > min, and I suspect that it's too fast because I do observe
>  > > tissue swelling sometimes.
>
>
>
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

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