[Histonet] mouse perfusion rate

[MKing]

Li,

Mouse cardiac output seems to be about 17 ml/min (e.g. 
www.transonic.com/mice1.shtml), you probably want to try for that to 
keep pressures close to physiological.
A syringe pump is pretty inexpensive and probably all you need.

Mike

----- Original Message -----
From: Li Zhang <dancingwing <@t> yahoo.com>
Date: Wednesday, March 17, 2010 14:59
Subject: [Histonet] question about mouse perfusion
To: histonet <@t> lists.utsouthwestern.edu

 > > My question is: can anyone give me a rough idea of how fast I
 > > should inject ( like ml/min). I think I've tried like 30 ml in 3
 > > min, and I suspect that it's too fast because I do observe
 > > tissue swelling sometimes.