[Histonet] Removing erythrocytes from fixed tissue

[Adam .]

Autofluorescence from RBCs is a common problem. See the archives for
discussion on how to fix this. For example,
http://lists.utsouthwestern.edu/pipermail/histonet/2009-December/048210.html

Adam

On Thu, Mar 18, 2010 at 10:35 AM, Ceri Allen <Ceri.Allen <@t> nottingham.ac.uk>wrote:

> Dear Histonetters,
>
>
>
> Does anyone know whether it is possible to remove erythrocytes from
> fixed tissue?
>
>
>
> Background: I am using an IgM antibody with a FITC conjugated secondary
> on 5 micron sections of formalin fixed tissue. I'm getting a lot of
> non-specific labelling of erythrocytes, but I'm not sure whether this is
> due to the IgM binding to the erythrocytes, or auto-fluorescence due to
> the tissue being formalin fixed. Changing the fixative is not possible
> as all the experimental tissue was collected a long time ago, So I'm
> looking for any other solutions.
>
>
>
> Any suggestions would be gratefully  received
>
>
>
> Ceri
>
>
>
> Research Technician
>
> School of Veterinary Medicine and Science
>
> Sutton Bonnington Campus
>
> University of Nottingham
>
>
>
> Tel. 0115 95 16459
>
> ceri.allen <@t> nottingham.ac.uk
>
>
>
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