[Histonet] Anti-human abs work on porcine tissue

[Jan Shivers]

I work on all species, porcine being one of them.  I've never done a % 
survey of the number of Abs made against human epitopes that also work on 
porcine tissue, but I can give you generalizations.

All of the rabbit polyclonal antibodies that I've tried have cross-reacted 
with porcine tissue.  The polyclonals that I use the most are the Abs 
against hormones, neuroendocrine markers, vasculature, astrocytes, etc.
Most of the mouse monoclonal antibodies that I've tried have cross-reacted 
with porcine tissue.  These would be the intermediate filaments, melanoma 
antigens, cell proliferating antigens, neuroendocrine markers, etc.
You may have more difficulty with leukocyte markers (CD markers).  They seem 
to have less homology with human markers than the above items, and not all 
cross-react, in my experience.  You only learn this by trial and error, 
unfortunately, since data about species cross-reactivity is rare.  I have 2 
polyclonal and 2 monoclonal CD markers that cross-react with porcine.  That 
is not to say that there aren't more, but that's all that I currently have 
worked up on porcine tissue.
I have not tried Abs like ER, PR, CEA, TTF, etc.
I've done species cross-reactivity studies with every antibody I have in 
stock (>80), so if you send me a list of the antibodies you're interested in 
(directly to me), I can let you know which ones cross-react with porcine 
tissue, and which vendor works for me.

Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu

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----- Original Message ----- 
From: "RICKY MATHIS" <cmmathis1 <@t> bellsouth.net>
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, March 17, 2010 8:15 PM
Subject: [Histonet] Anti-human abs work on porcine tissue


I work with some porcine tissues and it is sometimes difficult to find 
antibodies specific to pig. A doctor I work with asked some one he knew in 
Europe about using antibodies that are listed as working in human being used 
on porcine tissue. This person stated that there would be about a 10% chance 
that the anti-human antibodies would work on porcine tissues. The antibody 
companies mostly give the same "we did not try it on pig tissue" response. I 
understand that it is likely expensive to continue to test antibodies on a 
wide variety of animal tissues, so that is fine. But what do you guys think 
about the 10% chance? I would have said more than that.
Thank you in advance for your time,
Cathy
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