[Histonet] Anti-human abs work on porcine tissue
shive003 <@t> umn.edu
Thu Mar 18 07:48:53 CDT 2010
I work on all species, porcine being one of them. I've never done a %
survey of the number of Abs made against human epitopes that also work on
porcine tissue, but I can give you generalizations.
All of the rabbit polyclonal antibodies that I've tried have cross-reacted
with porcine tissue. The polyclonals that I use the most are the Abs
against hormones, neuroendocrine markers, vasculature, astrocytes, etc.
Most of the mouse monoclonal antibodies that I've tried have cross-reacted
with porcine tissue. These would be the intermediate filaments, melanoma
antigens, cell proliferating antigens, neuroendocrine markers, etc.
You may have more difficulty with leukocyte markers (CD markers). They seem
to have less homology with human markers than the above items, and not all
cross-react, in my experience. You only learn this by trial and error,
unfortunately, since data about species cross-reactivity is rare. I have 2
polyclonal and 2 monoclonal CD markers that cross-react with porcine. That
is not to say that there aren't more, but that's all that I currently have
worked up on porcine tissue.
I have not tried Abs like ER, PR, CEA, TTF, etc.
I've done species cross-reactivity studies with every antibody I have in
stock (>80), so if you send me a list of the antibodies you're interested in
(directly to me), I can let you know which ones cross-react with porcine
tissue, and which vendor works for me.
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN 55108
shive003 <@t> umn.edu
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----- Original Message -----
From: "RICKY MATHIS" <cmmathis1 <@t> bellsouth.net>
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, March 17, 2010 8:15 PM
Subject: [Histonet] Anti-human abs work on porcine tissue
I work with some porcine tissues and it is sometimes difficult to find
antibodies specific to pig. A doctor I work with asked some one he knew in
Europe about using antibodies that are listed as working in human being used
on porcine tissue. This person stated that there would be about a 10% chance
that the anti-human antibodies would work on porcine tissues. The antibody
companies mostly give the same "we did not try it on pig tissue" response. I
understand that it is likely expensive to continue to test antibodies on a
wide variety of animal tissues, so that is fine. But what do you guys think
about the 10% chance? I would have said more than that.
Thank you in advance for your time,
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