[Histonet] how to process calcified bone for cryomicrotomy

Thu Mar 18 05:27:46 CDT 2010

Hello Fellow Histologists,

I am writing because I need information on how to process calcified bone for cryomicrotomy. I am working on an animal bone study that will require cryosectioning of calcified bone to stain for fat (Oil Red O).  Does anyone have any information on this topic that would like to share?  A procedure, cryostat used, and mounting medium.

Thanks in advance,

Fanny

Maria V. De Guzman| Histology Technician I

Main     908.947.1100              Fax       908.947.1085

Direct   908.947.1482             Email   mdeguzman <@t> lifecell.com

Mobile  732.688.1386              www.Lifecell.com

LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, March 17, 2010 11:08 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 76, Issue 24

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Today's Topics:

   1. UNSUBSCRIBE (Vanessa Avalos)
   2. Exciting Histotech Job in New England!! (Alisha Dynan)
   3. Re: UNSUBSCRIBE (Kim.Donadio <@t> bhcpns.org)
   4. HT/HTL position Gainesville. FL (Konni Black)
   5. Re: proficiency testing for Muscle EHC- cont'd. (Barone, Carol )
   6. Spencer AO 820 microtome questions (Scott Parker)
   7. Job Openings in Phoenix, Arizona (Turner, Mark)
   8. Lab Assistant Position in Phoenix, AZ (Turner, Mark)
   9. Saffron/Eosin as a counterstain for H&E??? (sheila adey)
  10. RE: Saffron/Eosin as a counterstain for H&E??? (Liz Chlipala)
  11. NY License (Jeffrey Silverman)
  12. (no subject) (Jeffrey Silverman)
  13. CAP cancer protocols and checklists  (Carol Bryant)
  14. (no subject) (Jeffrey Silverman)
  15. Re: Removing Yellow color from slides refixed in Bouin's
      solution (Joseph Saby)
  16. RE: (no subject) (Sherwood, Margaret )
  17. RE: (no subject) (WILLIAM DESALVO)
  18. Re: Spencer AO 820 microtome questions (Michael Folsom)
  19. Re: Saffron/Eosin as a counterstain for H&E??? (John Kiernan)
  20. RE: Superfrost Plus Slides (Cathy)
  21. problem in IF stains (Aazath Raj)
  22. Accessioning Similar Samples (Breeden, Sara)
  23. Re: Accessioning Similar Samples (DKBoyd <@t> chs.net)
  24. RE: Accessioning Similar Samples (Weems, Joyce)
  25. Mc Coy fixative (Cynthia Pyse)

----------------------------------------------------------------------

Message: 1
Date: Tue, 16 Mar 2010 10:01:22 -0700
From: "Vanessa Avalos" <vavalos <@t> allergydermatology.com>
Subject: [Histonet] UNSUBSCRIBE
To: "'HISTONET LISTS'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001001cac52a$4ef4ba00$ecde2e00$@com>
Content-Type: text/plain;       charset="UTF-8"

I can only speak for myself but yes, I am aware of all steps to be taken in unsubscribing. Have followed them more than once but have been unsuccessful. So yes, my frustration as lead me to write IN ALL CAPS!!!!!  :)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Tuesday, March 16, 2010 7:53 AM
To: Dimitri Scholz
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] (no subject)

both of you are not allowed to use the internet anymore.
because you can't follow instructions.
WE CAN'T UNSUBSCRIBE YOU.  ONLY YOU CAN.

A state-imposed metaphysic or religion should be opposed, if necessary
at pistol-point.  We must fight for variety if we fight at all.  The
uniform is as dull as a sculptured egg.
--Lawrence Durrell, Consequential Data, "Balthazar"

On Tue, Mar 16, 2010 at 10:49 AM, Dimitri Scholz <dimitri.scholz <@t> ucd.ie> wrote:
> Me too!
>
> Dimitri Scholz, PhD, Dr. Sci.
> Director of Biological Imaging
> Conway Institute
> University College Dublin (UCD)
> Belfield, Dublin 4
> Ireland
> Tel: +353-1 716 6736
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vanessa
> Gutierrez
> Sent: 16 March 2010 14:21
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] (no subject)
>
> UNSUBSCRIBE ME FOR THE 5TH TIME!!! I HAVE FOLLOWED INSTRUCTIONS AND STILL AM
> ON THIS LIST! SORRY, I KNOW CAPS IS NOT ALLOWED SO LETS SEE IF BREAKING THE
> RULES GETS ME OFF!
>
> THANK YOU
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 2
Date: 16 Mar 2010 13:40:23 -0400
From: Alisha Dynan <alisha <@t> ka-recruiting.com>
Subject: [Histonet] Exciting Histotech Job in New England!!
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1516952045.1268761223703.JavaMail.cfservice <@t> webserver54>
Content-Type: text/plain; charset="utf-8"

Hi Histotechs,

I am a recruiter at a highly successful and well respected Healthcare recruiting firm.  I help place Lab Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities?  We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. I am currently working on some great job opportunities in various areas across the country.

One particular client that I am working with is a financially sound, medically advanced, full-service hospital in New England. My client is looking for both a HTL(ASCP) certified histotechnologist and a HT(ASCP) certified histotech for their hospital. Both positions would work a day shift. My client is offering a very competitive compensation package, full benefits, and relocation assistance. If interested, please email me a copy of your resume and follow-up with a call to learn more!

Below is a list of some of the great opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates.

Current Opportunities:

CT - Histology Operations Manager

NY - New York City - Histotech 3rd shift

NV -Las Vegas - Histotech 3rd shift

NV Las Vegas - Histology Supervisor - 3rd shift

PA - Cytology Supervisor

CA - Palm Springs - Histotech - 1st shift

CA - Southern - Histotech

VA - Histotech 2nd shift

NH - HTL and HT

GA - Atlanta - Histotech

GA - Atlanta - Pathology Coordinator (HT or CT)

NV - Pathologist Assistant

TN - Pathologist Assistant

ND - Pathologist Assistant

GA - Pathologist Assistant

If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is.

If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future.  I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country.

To view some additional opportunities please visit our website at www.ka-recruiting.com.

Sincerely,

Alisha (Taylor) Dynan, Founder

K.A. Recruiting, Inc.

Your Partner in Healthcare Recruiting

10 Post Office Square 8th Floor SOUTH

Boston, MA 02109

P: (617) 692-2949

F: (617) 507-8009

alisha <@t> ka-recruiting.com

www.ka-recruiting.com

------------------------------

Message: 3
Date: Tue, 16 Mar 2010 12:47:31 -0500
From: Kim.Donadio <@t> bhcpns.org
Subject: Re: [Histonet] UNSUBSCRIBE
To: "Vanessa Avalos" <vavalos <@t> allergydermatology.com>
Cc: 'HISTONET LISTS' <histonet <@t> lists.utsouthwestern.edu>,
        histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
        <OF178264B0.5BD13068-ON862576E8.006155FC-862576E8.0061BBE6 <@t> bhcpns.org>
Content-Type: text/plain;       charset="US-ASCII"

I have a suggestion. If you are extremely frustrated with getting all the
emails that come from here and you are having a hard time unsubscribing,
perhaps you could put a block on histonet so you cant receive emails from
them until you get unsubscribed?  Just a thought.

Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996

"Vanessa Avalos" <vavalos <@t> allergydermatology.com>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
03/16/2010 12:01 PM

To
"'HISTONET LISTS'" <histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] UNSUBSCRIBE

I can only speak for myself but yes, I am aware of all steps to be taken
in unsubscribing. Have followed them more than once but have been
unsuccessful. So yes, my frustration as lead me to write IN ALL CAPS!!!!!
:)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Emily
Sours
Sent: Tuesday, March 16, 2010 7:53 AM
To: Dimitri Scholz
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] (no subject)

both of you are not allowed to use the internet anymore.
because you can't follow instructions.
WE CAN'T UNSUBSCRIBE YOU.  ONLY YOU CAN.

A state-imposed metaphysic or religion should be opposed, if necessary
at pistol-point.  We must fight for variety if we fight at all.  The
uniform is as dull as a sculptured egg.
--Lawrence Durrell, Consequential Data, "Balthazar"

On Tue, Mar 16, 2010 at 10:49 AM, Dimitri Scholz <dimitri.scholz <@t> ucd.ie>
wrote:
> Me too!
>
> Dimitri Scholz, PhD, Dr. Sci.
> Director of Biological Imaging
> Conway Institute
> University College Dublin (UCD)
> Belfield, Dublin 4
> Ireland
> Tel: +353-1 716 6736
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vanessa
> Gutierrez
> Sent: 16 March 2010 14:21
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] (no subject)
>
> UNSUBSCRIBE ME FOR THE 5TH TIME!!! I HAVE FOLLOWED INSTRUCTIONS AND
STILL AM
> ON THIS LIST! SORRY, I KNOW CAPS IS NOT ALLOWED SO LETS SEE IF BREAKING
THE
> RULES GETS ME OFF!
>
> THANK YOU
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

-----------------------------------------
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------------------------------

Message: 4
Date: Tue, 16 Mar 2010 10:59:16 -0700
From: "Konni Black" <kblack <@t> digestivehlth.com>
Subject: [Histonet] HT/HTL position Gainesville. FL
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <DAB581F471B84B6788798F6A3570A878 <@t> digestivehlth.com>
Content-Type: text/plain;       charset="Windows-1252"

Fulltime HT/HTL opening in GI lab in Gainesville, Florida. Must have 3-5 years experience. Great lab. Flexible work schedule. Contact Konni Black at kblack <@t> digestivehlth.com or 253-503-2560.

------------------------------

Message: 5
Date: Tue, 16 Mar 2010 14:10:50 -0400
From: "Barone, Carol " <cbarone <@t> NEMOURS.ORG>
Subject: [Histonet] Re: proficiency testing for Muscle EHC- cont'd.
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <37E4BAC017F57141AF64FAA5AEB04CE8033A7AFC <@t> wlmmsx01.nemours.org>
Content-Type: text/plain;       charset="us-ascii"

Histonetter's I only received one reply on my question regarding "what
everyone is doing for proficiency testing of enzyme HC staining for
muscle"...espeically for CLIA.compliance, now that I will lose my
indepent reviewer.

 My thanks to Jan Minchew for her reply. I am working from that for now,
though my sitiation somewhat different. .....but, I am finding it
particularly frustrating finding an answer to this question over the
last three months or more, that I have searched everywhere from the
histonet to the internet toevery friend I have doing EHC. Is it required
under CLIA? My auditor says yes..so I am trying to comply, though I
cannot fiind it specifically in any CLIA reg.

Who is the CLIA expert out there...and why is this not included in HQIP?
Can someone shed more light. Thx. CB

------------------------------

Message: 6
Date: Tue, 16 Mar 2010 14:20:02 -0400
From: Scott Parker <sparker <@t> vt.edu>
Subject: [Histonet] Spencer AO 820 microtome questions
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <ed777d3c1003161120l73339e82t8c699af45feb7749 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Dear all,

I have access to a Spencer AO 820 microtome (a "black beauty" in
Histo-parlance)  that is does not have a disposable blade holder nor a chuck
for holding paraffin cassettes. The microtome otherwise appears to be in
good condition. I would like to get suggestions for where I might be able to
purchase a chuck and disposable blade holder for this classic model.
Alternatively, would it be a better idea for me to put this piece of
equipment on display in a glass case and instead purchase a newer microtome.
My work involves relatively low volume, basic sectioning of paraffin blocks
for teaching and research. I don't need anything too fancy, just a microtome
that is reliable and appropriate for student researchers to use.

Thank you in advance for your responses.

Scott

Scott L. Parker, Ph.D.

Assistant Professor Biology

Coastal Carolina University

P.O. Box  261954

Conway, SC 29528-6054

Office Phone: (843)-349-2491

------------------------------

Message: 7
Date: Tue, 16 Mar 2010 11:52:43 -0700
From: "Turner, Mark" <mturner <@t> carisdx.com>
Subject: [Histonet] Job Openings in Phoenix, Arizona
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <C3B8B71FD81E9F418F7F4725046D395401B483F6 <@t> s-phx-exc01.PathologyPartners.intranet>

Content-Type: text/plain;       charset="us-ascii"

Hello Histonetters!

Caris MPI, a division of Caris Life Sciences, is looking for 2
histotechs to assist with a dramatically increasing workflow.  We are a
very dynamic IHC lab which is growing exponentially.  The positions are
day shift, Monday through Friday, with occasional weekends.  The
successful candidates will have HT (ASCP) certification, 1-2 years IHC
experience, preferably with the Ventana and Dako platforms, and will
enjoy being part of a fast paced, quality oriented team.  Qualification
in Immunohistochemistry (QIHC) is a plus.

Caris MPI is located in sunny Phoenix, AZ and will be moving to a brand
new lab in May 2010.  Equipment is state of the art, and there is a
corporate commitment toward quality.   Caris has had a presence in the
Phoenix area for 3 years and is continuing to grow.  The area is the 5th
largest metropolitan in the country and has available anything you need
to make life more interesting.

If you would like to grow with us, please submit your resume in
confidence to mturner <@t> carisdx.com.

Mark Turner, Ph. D., HT(ASCP) QIHC

Supervisor IHC

Target Now MPI

Caris Life Sciences

445 N. 5th Street

Phoenix, AZ  85004

Cell:   602-309-5084

Direct  602-358-8913

Fax:  602-358-8919

mturner <@t> carisdx.com

------------------------------

Message: 8
Date: Tue, 16 Mar 2010 11:57:47 -0700
From: "Turner, Mark" <mturner <@t> carisdx.com>
Subject: [Histonet] Lab Assistant Position in Phoenix, AZ
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <C3B8B71FD81E9F418F7F4725046D395401B483FF <@t> s-phx-exc01.PathologyPartners.intranet>

Content-Type: text/plain;       charset="us-ascii"

Caris MPI, a division of Caris Life Sciences, is looking to hire a Lab
Assistant for 2nd shift.  This will be a support position for our team
of histology professionals, and is a great entry level position into the
laboratory setting.  Some college is preferred, with a focus on the
sciences.  Opportunities for advancement abound, as this position is
open due to our previous lab assistant moving into a histotech role.

Caris MPI is located in sunny Phoenix, AZ and will be moving to a brand
new lab in May 2010.  Equipment is state of the art, and there is a
corporate commitment toward quality.   Caris has had a presence in the
Phoenix area for 3 years and is continuing to grow.  The area is the 5th
largest metropolitan in the country and has available anything you need
to make life more interesting.

If you would like to grow with us, please submit your resume in
confidence to mturner <@t> carisdx.com.

Mark Turner, Ph. D., HT(ASCP) QIHC

Supervisor IHC

Target Now MPI

Caris Life Sciences

445 N. 5th Street

Phoenix, AZ  85004

Cell:   602-309-5084

Direct  602-358-8913

Fax:  602-358-8919

mturner <@t> carisdx.com

------------------------------

Message: 9
Date: Tue, 16 Mar 2010 15:04:23 -0400
From: sheila adey <sheila_adey <@t> hotmail.com>
Subject: [Histonet] Saffron/Eosin as a counterstain for H&E???
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY129-W13E52F7359896731646558932D0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

Hello,

Our pathologist said that he say H&E slides with a counterstain that had some amount of saffron in it?

Is anyone familiar with this?

Sheila Adey HT MLT

_________________________________________________________________
Stay in touch.
http://go.microsoft.com/?linkid=9712959

------------------------------

Message: 10
Date: Tue, 16 Mar 2010 13:30:38 -0600
From: "Liz Chlipala" <liz <@t> premierlab.com>
Subject: RE: [Histonet] Saffron/Eosin as a counterstain for H&E???
To: "sheila adey" <sheila_adey <@t> hotmail.com>,
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <EE33BE5C905A3046A7FF8F58A64C8E4B100D7E <@t> server.PremierLab.local>
Content-Type: text/plain;       charset="us-ascii"

Its hematoxylin, ploxine and saffron.  Believe it or not it was the
stain that we used at my first job back in 1973.  We also fixed in
Zenkers too.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com

Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of sheila
adey
Sent: Tuesday, March 16, 2010 1:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Saffron/Eosin as a counterstain for H&E???

Hello,

Our pathologist said that he say H&E slides with a counterstain that had
some amount of saffron in it?

Is anyone familiar with this?

Sheila Adey HT MLT

_________________________________________________________________
Stay in touch.
http://go.microsoft.com/?linkid=9712959_________________________________
______________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 11
Date: Tue, 16 Mar 2010 14:07:54 -0700 (PDT)
From: Jeffrey Silverman <pathmaster <@t> yahoo.com>
Subject: [Histonet] NY License
To: rmoody <@t> ameripath.com
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <12350.35531.qm <@t> web111103.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=us-ascii

New York State Education Department- google it and you'll see a link for licensed professionals.

Jeff Silverman

------------------------------

Message: 12
Date: Tue, 16 Mar 2010 14:14:57 -0700 (PDT)
From: Jeffrey Silverman <pathmaster <@t> yahoo.com>
Subject: [Histonet] (no subject)
To: cscampbe <@t> uci.edu
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <161299.62124.qm <@t> web111104.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Don't know what you mean by rinses, but 5 to at most 10 minutes in gently running tap water should do the trick. If not, the lithium carbonate is what's prescribed.

Jeff Silverman

------------------------------

Message: 13
Date: Tue, 16 Mar 2010 17:19:33 -0400
From: Carol Bryant <cbrya <@t> lexclin.com>
Subject: [Histonet] CAP cancer protocols and checklists
To: "Histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF0567DDC388 <@t> EXCHANGESB>
Content-Type: text/plain; charset="us-ascii"

I am interested in how you are implementing the CAP cancer protocols in your laboratory?  Are you using synoptic reporting, dictating from the checklists, etc?  Any information you can provide would be greatly appreciated.
Thank you in advance for your comments and input.

Carol

Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Pathology Services
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya <@t> lexclin.com

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------------------------------

Message: 14
Date: Tue, 16 Mar 2010 14:23:45 -0700 (PDT)
From: Jeffrey Silverman <pathmaster <@t> yahoo.com>
Subject: [Histonet] (no subject)
To: Allison_Scott <@t> hchd.tmc.edu
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <190750.38557.qm <@t> web111106.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi Allison-
That's our policy- don't accession like biopsies back to back.
But if it is unavoidable, you can mark the second like biopsy case with a dab of yellow margin ink on at least one piece in each block and dictate that into the gross.? ie One fragment is marked in yellow for positive patient identification.

Jeff Silverman- busy PA

------------------------------

Message: 15
Date: Tue, 16 Mar 2010 14:25:01 -0700 (PDT)
From: Joseph Saby <saby_joseph_a <@t> yahoo.com>
Subject: Re: [Histonet] Removing Yellow color from slides refixed in
        Bouin's solution
To: Cynthia Pyse <cpyse <@t> x-celllab.com>, cscampbe <@t> uci.edu,       HistoNet
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <619715.30232.qm <@t> web113816.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Running tap water for 10 minutes should do the trick.

Joe Saby

________________________________
From: Cynthia Pyse <cpyse <@t> x-celllab.com>
To: cscampbe <@t> uci.edu; HistoNet <histonet <@t> lists.utsouthwestern.edu>
Sent: Tue, March 16, 2010 10:52:20 AM
Subject: RE: [Histonet] Removing Yellow color from slides refixed in Bouin's solution

I just place the slides into 80% ETOH for 2 minutes.
Cindy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
cscampbe <@t> uci.edu
Sent: Monday, March 15, 2010 7:42 PM
To: HistoNet
Subject: [Histonet] Removing Yellow color from slides refixed in Bouin's
solution

Hi Histonet,

After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it
very difficult to remove the yellow coloring. Rinses with water are not
doing the trick. Does anyone have some advice on how to bring the slides
back to a clear color so that I may proceed with the Masson Trichrome
procedure?

Thanks!
-Colin

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Message: 16
Date: Tue, 16 Mar 2010 17:34:15 -0400
From: "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG>
Subject: RE: [Histonet] (no subject)
To: "Jeffrey Silverman" <pathmaster <@t> yahoo.com>, <cscampbe <@t> uci.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <073AE2BEA1C2BA4A8837AB6C4B943D9703E240DD <@t> PHSXMB30.partners.org>
Content-Type: text/plain; charset="us-ascii"

We also just wash in running tap water and the yellow stain disappears.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jeffrey
Silverman
Sent: Tuesday, March 16, 2010 5:15 PM
To: cscampbe <@t> uci.edu
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Don't know what you mean by rinses, but 5 to at most 10 minutes in gently
running tap water should do the trick. If not, the lithium carbonate is what's
prescribed.

Jeff Silverman

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Message: 17
Date: Tue, 16 Mar 2010 18:33:50 -0600
From: WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>
Subject: RE: [Histonet] (no subject)
To: <pathmaster <@t> yahoo.com>, <allison_scott <@t> hchd.tmc.edu>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU103-W23701C4208D2F9A987D432912C0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

An additional method to use to create positive identification when accessioning and grossing like specimens together when you must or just to increase the indetification factor for all specimens:

Use the Davidson marking system and have the grossing person rotate the colors used to mark margins or small tissue by case and/or specimen and of course dictate to specific color used. Once they are in the habit, no two cases or specimens will have the same ink color and you increase quality assurance.

William DeSalvo, B.S., HTL(ASCP)

> Date: Tue, 16 Mar 2010 14:23:45 -0700
> From: pathmaster <@t> yahoo.com
> To: Allison_Scott <@t> hchd.tmc.edu
> CC: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] (no subject)
>
> Hi Allison-
> That's our policy- don't accession like biopsies back to back.
> But if it is unavoidable, you can mark the second like biopsy case with a dab of yellow margin ink on at least one piece in each block and dictate that into the gross.  ie One fragment is marked in yellow for positive patient identification.
>
> Jeff Silverman- busy PA
>
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Message: 18
Date: Tue, 16 Mar 2010 18:53:05 -0600
From: Michael Folsom <mwfolsom <@t> rgbio.com>
Subject: Re: [Histonet] Spencer AO 820 microtome questions
To: Scott Parker <sparker <@t> vt.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1268787185.6216.14.camel <@t> chico.322tulane.org>
Content-Type: text/plain; charset="UTF-8"

Scott:

Does the chuck have a clamp in it?  If so soak a piece of soft wood that
fits in the chuck in 100% ETOH (pulling a vacuum on it would help) and
transition into paraffin.  Once its soaked in paraffin pull it out and
allow to solidify.  Your tissue will need to be flat embedded in a
simple boat.  Use a razor blade to cut it out but leave a good bit of
paraffin behind and around it. Once its cool use an artists pallet knife
(small) to melt the surface of the paraffin on the wood and the back of
the block and weld in place with the melted paraffin.

Re: the knife - they make a knife holder that holds either hi or low
profile disposable blades which fits in the microtome's knife holder.

One thing about the old AO - they are almost indestructible but never
back up the crank because it can strip the gears.

Good luck -

Mike
mwfolsom <@t> rgbio.cm

On Tue, 2010-03-16 at 14:20 -0400, Scott Parker wrote:
> Dear all,
>
> I have access to a Spencer AO 820 microtome (a "black beauty" in
> Histo-parlance)  that is does not have a disposable blade holder nor a chuck
> for holding paraffin cassettes. The microtome otherwise appears to be in
> good condition. I would like to get suggestions for where I might be able to
> purchase a chuck and disposable blade holder for this classic model.
> Alternatively, would it be a better idea for me to put this piece of
> equipment on display in a glass case and instead purchase a newer microtome.
> My work involves relatively low volume, basic sectioning of paraffin blocks
> for teaching and research. I don't need anything too fancy, just a microtome
> that is reliable and appropriate for student researchers to use.
>
> Thank you in advance for your responses.
>
> Scott
>
>
> Scott L. Parker, Ph.D.
>
> Assistant Professor Biology
>
> Coastal Carolina University
>
> P.O. Box  261954
>
> Conway, SC 29528-6054
>
>
>
> Office Phone: (843)-349-2491
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
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------------------------------

Message: 19
Date: Tue, 16 Mar 2010 23:05:55 -0400
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] Saffron/Eosin as a counterstain for H&E???
To: sheila adey <sheila_adey <@t> hotmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <fbb4e5315571.4ba00ed3 <@t> uwo.ca>
Content-Type: text/plain; CHARSET=US-ASCII

There is
Garvey,W (1991): Modification of the Mayer hematoxylin stain. J. Histotechnol. 14, 163-165.
The title is rather confusing. Her method included a modified composition of Mayer's haemalum (ethanol instead of chloral hydrate, and less alum), and instead of eosin Y the counterstain was a solution containing phloxin B (CI45410, Acid red 92) and saffron (which is CI 75100, Natural yellow 6). The author stated that saffron bought from a health food store was OK and much less expensive than that bought from a lab vendor.
     This counterstain was used by Rostoker et al (2001) Nephrology Dialysis Transplantation 16:513-517 in a study of intestinal pathology in glomerulonephritis.

John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: sheila adey <sheila_adey <@t> hotmail.com>
Date: Tuesday, March 16, 2010 15:05
Subject: [Histonet] Saffron/Eosin as a counterstain for H&E???
To: histonet <@t> lists.utsouthwestern.edu

>
> Hello,
>
> Our pathologist said that he say H&E slides with a counterstain
> that had some amount of saffron in it?
>
> Is anyone familiar with this?
>
> Sheila Adey HT MLT
>
>
>
> _________________________________________________________________
> Stay in touch.
> http://go.microsoft.com/?linkid=9712959_______________________________________________
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------------------------------

Message: 20
Date: Tue, 16 Mar 2010 20:34:43 -0700
From: "Cathy" <cfitz <@t> 007group.com>
Subject: RE: [Histonet] Superfrost Plus Slides
To: "'Fahy, Claire'" <Claire.Fahy <@t> agriculture.gov.ie>,  "'Lynn Lee'"
        <lynnlee2010 <@t> live.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <FB51FD708C6F4CF3B8BD1DA7BD8B0143 <@t> your8ba846406f>
Content-Type: text/plain;       charset="iso-8859-1"

Hi Claire,

We experienced problems with our IHC staining the fall of 2008 and it was
traced back to a problem with particular lot numbers of the Superfrost
slides.  It was difficult to track down due to the inconsistency of the
problem and the variation in the problem.  We finally spoke with our Ventana
technical specialist and she was able to tie our problem to problems that
other facilities were experiencing.
You could try contacting your Ventana technical resource person; they may be
able to help.

One thing we found out was that the Superfrost slides are manufactured at
one site and distributed to vendors under their own name.

Cathy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Fahy, Claire
Sent: Tuesday, March 16, 2010 4:05 AM
To: Lynn Lee
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Superfrost Plus Slides

Hi Lynn

Yes, our instrument is decontaminated and cleaned as per manual.

There was some old threads on Histonet where some people had got varying
staining between runs so I was wondering if anybody had found a solution to
this problem.Maybe it is simply the slides?
-----Original Message-----
From: Lynn Lee [mailto:lynnlee2010 <@t> live.com]
Sent: 16 March 2010 10:43
To: Fahy, Claire
Subject: RE: [Histonet] Superfrost Plus Slides

You could have contamination in the instrument. Has it been decontaminated
quarterly per the instruction manual? I have never heard of uneven staining
from plus slides.

> Date: Tue, 16 Mar 2010 10:16:38 +0000
> From: Claire.Fahy <@t> agriculture.gov.ie
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Superfrost Plus Slides
>
> Hi
>
> I know this is in reply to an old threat but we are experiencing varying
and uneven staining of our sections on the Ventana Benchmark.It is not
confined to a perticular anitbody and doesn't happen all the time either.
>
> We have been told that it may be the slides.We currently use Thermo
Scientific (Menzel-Glaser) superfrost plus slides.Does anyone use the
Ventana IHC slides?
>
> Has anyone has experienced similar problems?If so,what have you done to
resolve them.
>
> Thanks
>
> Claire fahy
> Lab analyst,
> Histopatholgy Lab
>
> Department of Agriculture, Fisheries and Food
>
> The information contained in this email and in any attachments is
confidential and is designated solely for the attention and use of the
intended recipient(s). This information may be subject to legal and
professional privilege. If you are not an intended recipient of this email,
you must not use, disclose, copy, distribute or retain this message or any
part of it. If you have received this email in error, please notify the
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>
> An Roinn Talmha?ochta, Iascaigh agus Bia
>
> T? an t-eolais san r?omhphost seo, agus in aon ceangl?in leis, faoi
phribhl?id agus faoi r?n agus le h-aghaigh an seola? amh?in. D?fh?adfadh
?bhar an seoladh seo bheith faoi phribhl?id profisi?nta n? dl?thi?il. Mura
tusa an seola? a bh? beartaithe leis an r?omhphost seo a fh?il, t? cosc air,
n? aon chuid de, a ?s?id, a ch?ipe?l, n? a scaoileadh. M? th?inig s? chugat
de bharr dearmad, t?igh i dteagmh?il leis an seolt?ir agus scrios an t-?bhar
? do r?omhaire le do thoil.
>
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T? an t-eolais san r?omhphost seo, agus in aon ceangl?in leis, faoi
phribhl?id agus faoi r?n agus le h-aghaigh an seola? amh?in. D?fh?adfadh
?bhar an seoladh seo bheith faoi phribhl?id profisi?nta n? dl?thi?il. Mura
tusa an seola? a bh? beartaithe leis an r?omhphost seo a fh?il, t? cosc air,
n? aon chuid de, a ?s?id, a ch?ipe?l, n? a scaoileadh. M? th?inig s? chugat
de bharr dearmad, t?igh i dteagmh?il leis an seolt?ir agus scrios an t-?bhar
? do r?omhaire le do thoil.
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------------------------------

Message: 21
Date: Wed, 17 Mar 2010 10:20:39 +0530
From: Aazath Raj <aazath <@t> hotmail.com>
Subject: [Histonet] problem in IF stains
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT137-w3417B9B444684F7A1B0FCAC82C0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

Dear Friends,

             I am having a problem in IF stains on Renal Bx.In direct immunofloresence on the frozen sections does not have disered contrast and many of the cases its falsely negative.

Can you people tell me the possible mistake which may cause these kind of effects on the IF stain.

I dont see any gross mistakes on the Frozen sections after sectioning.All the temperature and other things are fine on the cryostate, but my pathologist feels that there may be any problem in section.will that have any effects on the IF stains..

Aazathraj.

Technical Officer.

Apollo Hospitals

India.

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Message: 22
Date: Wed, 17 Mar 2010 06:04:10 -0600
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Accessioning Similar Samples
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <4D14F0FC9316DD41972D5F03C070908B02E46F82 <@t> nmdamailsvr.nmda.ad.nmsu.edu>

Content-Type: text/plain;       charset="us-ascii"

Regarding the need to avoid accessioning similar samples consecutively,
I addressed this last week with my bosses.  Specifically, I asked that
spleen specimens not be accessioned consecutively (because of shattering
and static and the possibility of fragments being carried over); my
response was that I should just not CUT the blocks consecutively.  Not
the point I was trying to make but typical in some cases when addressing
the issues histotechs face, true?

Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106

505-841-2576

------------------------------

Message: 23
Date: Wed, 17 Mar 2010 08:24:55 -0400
From: DKBoyd <@t> chs.net
Subject: Re: [Histonet] Accessioning Similar Samples
To: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>,
        histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
        <OFE1713805.E2A69779-ON852576E9.00441EB7-852576E9.0044331B <@t> chs.net>
Content-Type: text/plain;       charset="US-ASCII"

Sara,
Unfortunately the response you got is more common than I would like to
see.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F:
804-765-5582 l dkboyd <@t> chs.net

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Message: 24
Date: Wed, 17 Mar 2010 10:21:34 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: RE: [Histonet] Accessioning Similar Samples
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
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        <27648A6C5BC9B145813DF5182F83AB4507590C <@t> ITSSSXM01V1.one.ads.che.org>
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We have agar based markers - red, blue, green, yellow, orange, black,
that we include in the block for similar tissues - as we have several
that be separated. It is noted in the gross and confirmed at micro that
the markers are present. After we've gone through the colors once, we
put two, three, etc. It has worked well for us. J

Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax

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Message: 25
Date: Wed, 17 Mar 2010 11:00:35 -0400
From: "Cynthia Pyse" <cpyse <@t> x-celllab.com>
Subject: [Histonet] Mc Coy fixative
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001cac5e2$99201da0$cb6058e0$@com>
Content-Type: text/plain;       charset="us-ascii"

Hi Histonetters

I am having a senior moment. Can anyone tell me what McCoy fixative is made
of and what it is used for? Thanks in advance for the help.

Cindy Pyse, CLT, HT (ASCP)

Histology Supervisor

X-Cell Laboratories

e-mail cpyse <@t> x-celllab.com

------------------------------

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End of Histonet Digest, Vol 76, Issue 24
****************************************