[Histonet] Anti-human abs work on porcine tissue
anonwums1 <@t> gmail.com
Wed Mar 17 20:38:01 CDT 2010
I routine use anti-human antibodies to stain mouse tissue, so it can be
done. It completely depends on the antigen they used to immunize. If the
human and pig proteins are highly homologous or identical, then it has a
good chance of working. If they're not, you're in uncharted territories. If
you ask nicely, most companies will tell you what antigen or antigen
fragment they used, but most don't actively advertise this.
On Wed, Mar 17, 2010 at 8:15 PM, RICKY MATHIS <cmmathis1 <@t> bellsouth.net>wrote:
> I work with some porcine tissues and it is sometimes difficult to find
> antibodies specific to pig. A doctor I work with asked some one he knew in
> Europe about using antibodies that are listed as working in human being used
> on porcine tissue. This person stated that there would be about a 10%
> chance that the anti-human antibodies would work on porcine tissues. The
> antibody companies mostly give the same "we did not try it on pig tissue"
> response. I understand that it is likely expensive to continue to test
> antibodies on a wide variety of animal tissues, so that is fine. But what
> do you guys think about the 10% chance? I would have said more than that.
> Thank you in advance for your time,
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