[Histonet] problem in IF stains

[Aazath Raj]

Dear Friends,

             I am having a problem in IF stains on Renal Bx.In direct immunofloresence on the frozen sections does not have disered contrast and many of the cases its falsely negative.

Can you people tell me the possible mistake which may cause these kind of effects on the IF stain.

I dont see any gross mistakes on the Frozen sections after sectioning.All the temperature and other things are fine on the cryostate, but my pathologist feels that there may be any problem in section.will that have any effects on the IF stains..

 

Aazathraj.

Technical Officer.

Apollo Hospitals

India.
 		 	   		  
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