[Histonet] Superfrost Plus Slides

[Cathy]

Hi Claire,

We experienced problems with our IHC staining the fall of 2008 and it was
traced back to a problem with particular lot numbers of the Superfrost
slides.  It was difficult to track down due to the inconsistency of the
problem and the variation in the problem.  We finally spoke with our Ventana
technical specialist and she was able to tie our problem to problems that
other facilities were experiencing.  
You could try contacting your Ventana technical resource person; they may be
able to help.

One thing we found out was that the Superfrost slides are manufactured at
one site and distributed to vendors under their own name.

Cathy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Fahy, Claire
Sent: Tuesday, March 16, 2010 4:05 AM
To: Lynn Lee
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Superfrost Plus Slides

Hi Lynn

Yes, our instrument is decontaminated and cleaned as per manual.

There was some old threads on Histonet where some people had got varying
staining between runs so I was wondering if anybody had found a solution to
this problem.Maybe it is simply the slides?
-----Original Message-----
From: Lynn Lee [mailto:lynnlee2010 <@t> live.com]
Sent: 16 March 2010 10:43
To: Fahy, Claire
Subject: RE: [Histonet] Superfrost Plus Slides

You could have contamination in the instrument. Has it been decontaminated
quarterly per the instruction manual? I have never heard of uneven staining
from plus slides.






> Date: Tue, 16 Mar 2010 10:16:38 +0000
> From: Claire.Fahy <@t> agriculture.gov.ie
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Superfrost Plus Slides
>
> Hi
>
> I know this is in reply to an old threat but we are experiencing varying
and uneven staining of our sections on the Ventana Benchmark.It is not
confined to a perticular anitbody and doesn't happen all the time either.
>
> We have been told that it may be the slides.We currently use Thermo
Scientific (Menzel-Glaser) superfrost plus slides.Does anyone use the
Ventana IHC slides?
>
> Has anyone has experienced similar problems?If so,what have you done to
resolve them.
>
> Thanks
>
> Claire fahy
> Lab analyst,
> Histopatholgy Lab
>
> Department of Agriculture, Fisheries and Food
>
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An Roinn Talmhaíochta, Iascaigh agus Bia

Tá an t-eolais san ríomhphost seo, agus in aon ceangláin leis, faoi
phribhléid agus faoi rún agus le h-aghaigh an seolaí amháin. D’fhéadfadh
ábhar an seoladh seo bheith faoi phribhléid profisiúnta nó dlíthiúil. Mura
tusa an seolaí a bhí beartaithe leis an ríomhphost seo a fháil, tá cosc air,
nó aon chuid de, a úsáid, a chóipeál, nó a scaoileadh. Má tháinig sé chugat
de bharr dearmad, téigh i dteagmháil leis an seoltóir agus scrios an t-ábhar
ó do ríomhaire le do thoil.
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