[Histonet] Superfrost Plus Slides

[Fahy, Claire]

Hi Lynn

Yes, our instrument is decontaminated and cleaned as per manual.

There was some old threads on Histonet where some people had got varying staining between runs so I was wondering if anybody had found a solution to this problem.Maybe it is simply the slides?
-----Original Message-----
From: Lynn Lee [mailto:lynnlee2010 <@t> live.com]
Sent: 16 March 2010 10:43
To: Fahy, Claire
Subject: RE: [Histonet] Superfrost Plus Slides

You could have contamination in the instrument. Has it been decontaminated quarterly per the instruction manual? I have never heard of uneven staining from plus slides.






> Date: Tue, 16 Mar 2010 10:16:38 +0000
> From: Claire.Fahy <@t> agriculture.gov.ie
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Superfrost Plus Slides
>
> Hi
>
> I know this is in reply to an old threat but we are experiencing varying and uneven staining of our sections on the Ventana Benchmark.It is not confined to a perticular anitbody and doesn't happen all the time either.
>
> We have been told that it may be the slides.We currently use Thermo Scientific (Menzel-Glaser) superfrost plus slides.Does anyone use the Ventana IHC slides?
>
> Has anyone has experienced similar problems?If so,what have you done to resolve them.
>
> Thanks
>
> Claire fahy
> Lab analyst,
> Histopatholgy Lab
>
> Department of Agriculture, Fisheries and Food
>
> The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege. If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s).
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> An Roinn Talmhaíochta, Iascaigh agus Bia
>
> Tá an t-eolais san ríomhphost seo, agus in aon ceangláin leis, faoi phribhléid agus faoi rún agus le h-aghaigh an seolaí amháin. D’fhéadfadh ábhar an seoladh seo bheith faoi phribhléid profisiúnta nó dlíthiúil. Mura tusa an seolaí a bhí beartaithe leis an ríomhphost seo a fháil, tá cosc air, nó aon chuid de, a úsáid, a chóipeál, nó a scaoileadh. Má tháinig sé chugat de bharr dearmad, téigh i dteagmháil leis an seoltóir agus scrios an t-ábhar ó do ríomhaire le do thoil.
>
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An Roinn Talmhaíochta, Iascaigh agus Bia

Tá an t-eolais san ríomhphost seo, agus in aon ceangláin leis, faoi phribhléid agus faoi rún agus le h-aghaigh an seolaí amháin. D’fhéadfadh ábhar an seoladh seo bheith faoi phribhléid profisiúnta nó dlíthiúil. Mura tusa an seolaí a bhí beartaithe leis an ríomhphost seo a fháil, tá cosc air, nó aon chuid de, a úsáid, a chóipeál, nó a scaoileadh. Má tháinig sé chugat de bharr dearmad, téigh i dteagmháil leis an seoltóir agus scrios an t-ábhar ó do ríomhaire le do thoil.