[Histonet] Removing Yellow color from slides refixed in Bouin's solution

Geoff McAuliffe mcauliff <@t> umdnj.edu
Tue Mar 16 09:13:48 CDT 2010

A few drops of saturated aqueous lithium carbonate added to the 70% 
alcohol step will do the job nicely.


cscampbe <@t> uci.edu wrote:
> Hi Histonet,
> After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it
> very difficult to remove the yellow coloring. Rinses with water are not
> doing the trick. Does anyone have some advice on how to bring the slides
> back to a clear color so that I may proceed with the Masson Trichrome
> procedure?
> Thanks!
> -Colin
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu

More information about the Histonet mailing list