[Histonet] Removing Yellow color from slides refixed in Bouin's solution

Geoff McAuliffe mcauliff <@t> umdnj.edu
Tue Mar 16 09:13:48 CDT 2010


A few drops of saturated aqueous lithium carbonate added to the 70% 
alcohol step will do the job nicely.

Geoff

cscampbe <@t> uci.edu wrote:
> Hi Histonet,
>
> After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it
> very difficult to remove the yellow coloring. Rinses with water are not
> doing the trick. Does anyone have some advice on how to bring the slides
> back to a clear color so that I may proceed with the Masson Trichrome
> procedure?
>
> Thanks!
> -Colin
>
>
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>
>   


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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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mcauliff <@t> umdnj.edu
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