[Histonet] Removing Yellow color from slides refixed in Bouin'ssolution

Tony Henwood AnthonyH <@t> chw.edu.au
Mon Mar 15 19:03:00 CDT 2010

Place slides in saturated lithium carbonate for a few minutes.
This should do the trick


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
cscampbe <@t> uci.edu
Sent: Tuesday, 16 March 2010 10:42 AM
To: HistoNet
Subject: [Histonet] Removing Yellow color from slides refixed in

Hi Histonet,

After placing slides in Bouin's for 1 hour at 56 degrees, I am finding
it very difficult to remove the yellow coloring. Rinses with water are
not doing the trick. Does anyone have some advice on how to bring the
slides back to a clear color so that I may proceed with the Masson
Trichrome procedure?


Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.

More information about the Histonet mailing list