[Histonet] Removing Yellow color from slides refixed in Bouin'ssolution

[Tony Henwood]

Place slides in saturated lithium carbonate for a few minutes.
This should do the trick

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
cscampbe <@t> uci.edu
Sent: Tuesday, 16 March 2010 10:42 AM
To: HistoNet
Subject: [Histonet] Removing Yellow color from slides refixed in
Bouin'ssolution


Hi Histonet,

After placing slides in Bouin's for 1 hour at 56 degrees, I am finding
it very difficult to remove the yellow coloring. Rinses with water are
not doing the trick. Does anyone have some advice on how to bring the
slides back to a clear color so that I may proceed with the Masson
Trichrome procedure?

Thanks!
-Colin


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