SPAM-LOW: [Histonet] Re: Formalin vs alcoholic formalin

Patsy Ruegg pruegg <@t>
Sat Mar 13 10:09:25 CST 2010

Her2 loves alcohol and the use of it can cause false positive results.  Even
how the formalin is buffered matters, use only phosphate neutral buffered


Patsy Ruegg, HT(ASCP)QIHC
12635 Montview Blvd. Ste.215
Aurora, CO 80045
fax 720-859-4110

-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of Robert
Sent: Thursday, March 11, 2010 2:20 PM
To: histonet <@t>
Subject: SPAM-LOW: [Histonet] Re: Formalin vs alcoholic formalin

William (Bill) O'Donnell, HT (ASCP) QIHC, Lead Histologist, Good
Samaritan Hospital, Kearney, Nebraska asks:

>>Help! I think I know the answer, but need some rapid clarification. Is an
alcohol-formalin fixative acceptable for use in breast tissue, or does it
need to be an aqueous 10% NBF?<<

According to the FDA, the required fixation for the HER2 immunostain
on breast tissue is neutral buffered formalin - accept no substitutes!

I agree with them.

Bob Richmond
Samurai Pathologist
Knoxville TN

Histonet mailing list
Histonet <@t>

More information about the Histonet mailing list