SPAM-LOW: [Histonet] Re: Formalin vs alcoholic formalin

[Patsy Ruegg]

Her2 loves alcohol and the use of it can cause false positive results.  Even
how the formalin is buffered matters, use only phosphate neutral buffered
formalin.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert
Richmond
Sent: Thursday, March 11, 2010 2:20 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] Re: Formalin vs alcoholic formalin

William (Bill) O'Donnell, HT (ASCP) QIHC, Lead Histologist, Good
Samaritan Hospital, Kearney, Nebraska asks:

>>Help! I think I know the answer, but need some rapid clarification. Is an
alcohol-formalin fixative acceptable for use in breast tissue, or does it
need to be an aqueous 10% NBF?<<

According to the FDA, the required fixation for the HER2 immunostain
on breast tissue is neutral buffered formalin - accept no substitutes!

I agree with them.

Bob Richmond
Samurai Pathologist
Knoxville TN

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