[Histonet] hydrometer
Beth Austin
baustin <@t> cbgbiotech.com
Wed Mar 10 10:17:06 CST 2010
CBG Biotech offers hydrometers.
Regards,
Beth Austin
CBG Biotech
1-800-941-9484 ext 200
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
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Subject: Histonet Digest, Vol 76, Issue 11
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Today's Topics:
1. Helicobacter Pylori antibody (Chakib Boussahmain)
2. RE: Helicobacter Pylori antibody (McMahon, Loralee A)
3. RE: Helicobacter Pylori antibody (Sebree Linda A)
4. Tissue Tek (ricky hachy)
5. RE: Helicobacter Pylori antibody (Cynthia Pyse)
6. Re: Helicobacter Pylori antibody (Kim Tournear)
7. Re: Eosin too pink (Kim Tournear)
8. hydrometer (Vickroy, Jim)
9. Re: hydrometer (DKBoyd <@t> chs.net)
10. Region II mtg addiiton and up-date: (Barone, Carol )
11. p16 (Knutson, Deanne)
12. Re: Butyl alcohol (Rene J Buesa)
13. Masson Trichrome, Alligator Heart Tissue, colors turning out
purple (cscampbe <@t> uci.edu)
14. Need help with FISH staining protocol on frozen tissue
sections (Shen, Jinhui)
15. Re: p16 (Dana Settembre)
16. RE: p16 (Cynthia Pyse)
17. Masson Trichrome, Alligator Heart Tissue, colors turning out
purple (Durden, Kelley)
18. Microwave Tissue Processor Protocols (Green JumpyOne)
19. HistoDay (Amy Johnson)
20. Histology Professionals Day (Breeden, Sara)
21. Re: Histology Professionals Day (Nita Searcy)
22. Happy HPD to our "Unsung Heroes" (Alyssa Peterson)
23. Re: Need help with FISH staining protocol on frozen tissue
sections (Merced M Leiker)
24. RE: Histology Professionals Day (Beckham, Sharon)
25. RE: HistoDay (Marsh, Nannette)
26. RE: HistoDay (Marsh, Nannette)
27. Histo Professionals Day (Breeden, Sara)
----------------------------------------------------------------------
Message: 1
Date: Tue, 9 Mar 2010 10:45:54 -0800 (PST)
From: Chakib Boussahmain <chak_bou <@t> yahoo.com>
Subject: [Histonet] Helicobacter Pylori antibody
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <591938.93716.qm <@t> web58105.mail.re3.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hey Histonet,
Is anyone using Helicobacter pylori antibody? if so, can you tell me where
did you get it? and the protocol?
Any input will appreciated
Thank you so much.
Chakib HTL(ASCP)
MIT-DCM
------------------------------
Message: 2
Date: Tue, 9 Mar 2010 13:52:36 -0500
From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
Subject: RE: [Histonet] Helicobacter Pylori antibody
To: Chakib Boussahmain <chak_bou <@t> yahoo.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C27AA2A01CEF31469813089E226F582E63759C58 <@t> urmcms7.urmc-sh.rochester.edu>
Content-Type: text/plain; charset="us-ascii"
I have ordered mine from Cell Marque. We use it at 1/50 dilution with a 30
minute incubation at RT on the Dako Autostainers. Works great.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Chakib Boussahmain
[chak_bou <@t> yahoo.com]
Sent: Tuesday, March 09, 2010 1:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Helicobacter Pylori antibody
Hey Histonet,
Is anyone using Helicobacter pylori antibody? if so, can you tell me where
did you get it? and the protocol?
Any input will appreciated
Thank you so much.
Chakib HTL(ASCP)
MIT-DCM
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
Date: Tue, 9 Mar 2010 12:58:14 -0600
From: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Subject: RE: [Histonet] Helicobacter Pylori antibody
To: "Chakib Boussahmain" <chak_bou <@t> yahoo.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8C023B4AB999614BA4791BAEB26E27381BF7E4 <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="iso-8859-1"
Hi Chakib,
We use Ventana's (manufactured by Cell Marque) H. Pylori on their
instruments. The protocol is specific to the instruments but involves a
routine HIER step and Avidin/Biotin blocking. The antibody itself is
ready-to-use but is available from Cell Marque as a concentrate as well.
The protocol produces a very clean stain; essential since these bugs are so
small.
Hope this helps,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Chakib
Boussahmain
Sent: Tuesday, March 09, 2010 12:46 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Helicobacter Pylori antibody
Hey Histonet,
Is anyone using Helicobacter pylori antibody? if so, can you tell me where
did you get it? and the protocol? Any input will appreciated Thank you so
much. Chakib HTL(ASCP) MIT-DCM
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Tue, 9 Mar 2010 19:00:49 +0000
From: ricky hachy <elciba <@t> hotmail.com>
Subject: [Histonet] Tissue Tek
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT114-W552E106567733D196E0811CF340 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
I need the 2 parafin pots for a Tissue Tek/Sakura tissue processor working
or not .
Anyone has them ?
Regards
Ricky
_________________________________________________________________
Your E-mail and More On-the-Go. Get Windows Live Hotmail Free.
http://clk.atdmt.com/GBL/go/201469229/direct/01/
------------------------------
Message: 5
Date: Tue, 9 Mar 2010 14:16:08 -0500
From: "Cynthia Pyse" <cpyse <@t> x-celllab.com>
Subject: RE: [Histonet] Helicobacter Pylori antibody
To: "'Chakib Boussahmain'" <chak_bou <@t> yahoo.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001cabfbc$f93ecdc0$ebbc6940$@com>
Content-Type: text/plain; charset="iso-8859-1"
We purchase it from Biocare. Protocol: pretreatment Diva (Biocare) 20
minutes water bath 20 minutes cool down. Detection system Mach 4 (Biocare)
10 minutes H2O2, 10 minute primary antibody dilution 1:100, 5 minutes mouse
probe, 12 minutes polymer, 10 minutes DAB(Dako), 5 minutes
hematoxylin(Dako).
Works great.
Cindy
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Chakib
Boussahmain
Sent: Tuesday, March 09, 2010 1:46 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Helicobacter Pylori antibody
Hey Histonet,
Is anyone using Helicobacter pylori antibody? if so, can you tell me where
did you get it? and the protocol?
Any input will appreciated
Thank you so much.
Chakib HTL(ASCP)
MIT-DCM
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Tue, 9 Mar 2010 11:52:54 -0800 (PST)
From: Kim Tournear <kimtournear <@t> yahoo.com>
Subject: Re: [Histonet] Helicobacter Pylori antibody
To: Chakib Boussahmain <chak_bou <@t> yahoo.com>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <544545.25352.qm <@t> web54204.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
We use Cell Marque's Polyclonal H. Pylori (CMC435) at a 1:100 dilution on
the Ventana BenchMark for 32 minutes, Protease 1 for 12 minutes.
~Kim Tournear ~ HT (ASCP), QIHC (ASCP)
Histology Supervisor
Tucson Medical Center
Tucson, AZ
~Don't let your life end before it begins~
OU Rocks!!!!
________________________________
From: Chakib Boussahmain <chak_bou <@t> yahoo.com>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Tue, March 9, 2010 11:45:54 AM
Subject: [Histonet] Helicobacter Pylori antibody
Hey Histonet,
Is anyone using Helicobacter pylori antibody? if so, can you tell me where
did you get it? and the protocol?
Any input will appreciated
Thank you so much.
Chakib HTL(ASCP)
MIT-DCM
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Tue, 9 Mar 2010 12:00:43 -0800 (PST)
From: Kim Tournear <kimtournear <@t> yahoo.com>
Subject: Re: [Histonet] Eosin too pink
To: "Smith, Lesley" <Lesley.Smith <@t> ramsayhealth.co.uk>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <382091.74830.qm <@t> web54203.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
We add 100mL of 100% alcohol to 150mL of Eosin to tone it down each day.
~Kim Tournear ~ HT (ASCP), QIHC (ASCP)
Histology Supervisor
Tucson Medical Center
Tucson, AZ
~Don't let your life end before it begins~
OU Rocks!!!!
________________________________
From: "Smith, Lesley" <Lesley.Smith <@t> ramsayhealth.co.uk>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Tue, March 9, 2010 7:51:46 AM
Subject: [Histonet] Eosin too pink
Our Pathologists have, over the last few weeks, complained that the eosin is
too pink. We have not altered our procedures or reagents in any way. It
seems to be particularly bad in endoscopic biopsies. Thanks
Lesley Smith
Senior Biomedical Scientist Cellular Pathology
The Yorkshire Clinic
Bradford Road
Bingley
BD16 1TW
Switchboard: +44 1274 550600 ext 3348
Direct Dial: +44 1274 550800
http: www.ramsayhealth.co.uk <http://www.ramsayhealth.co.uk/>
************************************************
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------------------------------
Message: 8
Date: Tue, 9 Mar 2010 14:08:17 -0600
From: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
Subject: [Histonet] hydrometer
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<24A4826E8EF0964D86BC5317306F58A54257BC3090 <@t> mmc-mail.ad.mhsil.com>
Content-Type: text/plain; charset="us-ascii"
Anybody have any idea where to purchase a simple hydrometer for checking the
water content in alcohols? I found one at TBS but do not know if it will
fit our purposes since it says use with a specific recycler.
Thanks
James Vickroy BS, HT(ASCP)
Surgical and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046
________________________________
This message (including any attachments) contains confidential information
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------------------------------
Message: 9
Date: Tue, 9 Mar 2010 15:12:05 -0500
From: DKBoyd <@t> chs.net
Subject: Re: [Histonet] hydrometer
To: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
Cc: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OF34202695.26373C83-ON852576E1.006EEA59-852576E1.006EF94E <@t> chs.net>
Content-Type: text/plain; charset="US-ASCII"
We ordered ours from Fisher Scientific.
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
804-765-5582 l dkboyd <@t> chs.net
--------------------------------------------------------------------------
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is intended only for the use of the individual(s) and entity named
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------------------------------
Message: 10
Date: Tue, 9 Mar 2010 17:02:30 -0500
From: "Barone, Carol " <cbarone <@t> NEMOURS.ORG>
Subject: [Histonet] Region II mtg addiiton and up-date:
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<37E4BAC017F57141AF64FAA5AEB04CE8033A7AC2 <@t> wlmmsx01.nemours.org>
Content-Type: text/plain; charset="us-ascii"
Histonetter's, Region II up-date: Save the date for the
2010 Region II Symposium! June 10-12, 2010. Clarion Hotel, Altlantic
City, N.J ...Come for a day... or come for the weekend.
Excellent Speakers, new workshops, vendor fair and of course...there is
always the beach.
..(for studying, of course!). Stay tuned to the histonet, for more
information
regarding specific seminars and workshops.
More to come...
Information on registration will be posted on the histonet in April.
So keep up with the histonet...for lots of great networking.... and info
on the Region II Symposium
See you there. Carol Barone - Region II Director...Oh!...and keep
networking! It is the best educational tool of all!
------------------------------
Message: 11
Date: Tue, 9 Mar 2010 16:27:30 -0600
From: "Knutson, Deanne" <DKnutson <@t> primecare.org>
Subject: [Histonet] p16
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1E0E2B14C709174B8AC2BE0AE7F768338FE967C68A <@t> EXCHANGE2K7.staprimecare.org>
Content-Type: text/plain; charset="us-ascii"
Hello fellow Histonetters,
Does anyone run the antibody p16? If so, where do you purchase it from and
maybe you can share your protocol with me? Thank you in advance.
Deanne Knutson
Anatomic Pathology Supervisor
St. Alexius Medical Center
(701)-530-6730
dknutson <@t> primecare.org<mailto:dknutson <@t> primecare.org>
________________________________
This email may include confidential and privileged information. If this is
not intended for your use, please destroy immediately and contact the sender
of the message.
------------------------------
Message: 12
Date: Tue, 9 Mar 2010 14:43:54 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Butyl alcohol
To: ToddKrueger <Todd.Krueger <@t> bsci.com>, Michael Folsom
<mwfolsom <@t> rgbio.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <169396.76443.qm <@t> web65709.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
As an additional note: paraffin oil = mineral oil.
Reni J.
--- On Tue, 3/9/10, Michael Folsom <mwfolsom <@t> rgbio.com> wrote:
From: Michael Folsom <mwfolsom <@t> rgbio.com>
Subject: Re: [Histonet] Butyl alcohol
To: "Krueger, Todd" <Todd.Krueger <@t> bsci.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, March 9, 2010, 11:17 AM
Hi:
I'm not sure if this helps but Botanists have long used tert-butanol
(not N-butanol) to embed tissue in paraffin. This classic protocol was
popularized by D. A. Johansen in the mid 1930's and involves transition
from water to alcohol to t-butanol (I can supply more detailed info if
you need it). Once in 100% t-butanol tissue is transitioned to light
paraffin oil. The final stage involves filling some large shell vials
1/3 full with paraffin and letting them harden. Tissue plus paraffin
oil is added to the vials (volume of paraffin oil + tissue should be be
approximately equal to the amount of paraffin). Vials are now placed in
an oven for paraffin to melt and infiltration to begin. Besides doing
enough changes of paraffin to infiltrate the tissue and remove all the
paraffin oil that's about it. From there its the old embed, section and
enjoy!
Hope this helps -
Mike Folsom
Rio Grande Biological
mwfolsom <@t> rgbio.com
On Mon, 2010-03-08 at 09:00 -0600, Krueger, Todd wrote:
> Does anyone have a procedure for processing with N-butyl Alcohol. We
> need to find a procedure w/o xylene.
> Thanks
>
> Todd Krueger
>
> HTL(ASCP)CM
>
> Boston Scientific
>
> 2 Scimed Place, P121
>
> Osseo, MN 55311
>
> Phone: 763-694-5709
>
> Fax: 763-694-5505
>
> e-mail: todd.krueger <@t> bsci.com
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 13
Date: Tue, 9 Mar 2010 17:06:21 -0800
From: cscampbe <@t> uci.edu
Subject: [Histonet] Masson Trichrome, Alligator Heart Tissue, colors
turning out purple
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<88928dfe83ca47dbf12638e73258f4a1.squirrel <@t> webmail.uci.edu>
Content-Type: text/plain;charset=iso-8859-1
To Histonet:
This is my first time mailing the histonet community. Coincidentally, this
is my first venture into histology. My lab is conducting research on
alligator heart tissue. At the moment, I am attempting to stain my slides
using Masson Trichrome to differentiate muscle from connective tissue.
Results have not been optimal. The slides turn out to be more purple than
I had anticipated (comparing to pictures from journals). The red can be
told apart from the teal, but the colors are not bright.
Here is the procedure I am using:
1. Deparaffinize and hydrate to water
2. Iron Alum, 30 min
3. Wash in Dis. Water, 5 min
4. Hematoxylin stain, 30 min
5. Wash in Dis. Water, 5 min
6. Differentiate in saturated aq. picric acid, 1 min
7. Wash in dis. water, 10 min
8. Stain in acid fuschin, 5 min
9. Rinse in water
10. Stain in Ponceau de Xylidene, 5 min
11. Rinse in water
12. Differentiate in Phosphomolybdic acid, 5 min
13. Fast Green, 2 min
14. Differentiate in 1% aq. acetic acid, 5 min
15. Dehydrate
16. xylene (two washes, 3 min each)
I would greatly appreciate any advice on how to modify my procedure to
better stain the cardiac tissue. Or should I be using a different
trichrome method better suited to this particular tissue?
Thank you!
-Colin
------------------------------
Message: 14
Date: Wed, 10 Mar 2010 00:44:08 -0600
From: "Shen, Jinhui" <jinhui <@t> uta.edu>
Subject: [Histonet] Need help with FISH staining protocol on frozen
tissue sections
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F1CF91345A31FC4795BCDE7836D0EAB54F46F66A14 <@t> MAVMAIL2.uta.edu>
Content-Type: text/plain; charset="us-ascii"
Hi everyone,
Has anyone done FISH staining on mice tissue frozen sections for Y
chromosome? I need help with a successful protocol.
Recently I started to do FISH staining on mice liver frozen sections for
chromosome Y, but got no positive results even on male samples yet. The
probe I used was IDMF1057 Green label Chr Y probe from ID Labs Inc.
(www.idlabs.com). They have a protocol online probably for cultured cells
(http://www.idlabs.com/product/datasheets/IDMF1057.pdf?PHPSESSID=b39f16507cf
76b69495166c651f0b52c) which I haven't tried yet. I followed the following
protocol for frozen sections:
1. Fix sections in 4% PFA at 4C for 10 mins, then wash in PBS.
2. Treat the sections with 0.01% Pepsin, 37C 10min, wash in PBS.
3. Dehydrate the slides using 70% Ethanol (2minsX2), 90% Ethanol (2mins X2),
100% Ethanol (5 mins X1).Then Age the slides at 65C for one hour.
4. Mix the probe and hybridization buffer. Denature the probe by incubate it
at 65C for 10 mins, then hold it at 37C for 30 - 60 mins.
5. Denature the sections by incubate them in denaturing solution
(formamide:2XSSC, 70:30) at 75C for 2 mins.
6. Quench the slides in 4C 70% Ethanol for 4 mins. Dehydrate the slides as
step 3.
7. Apply the probe and hybridize at 37C overnight.
8. Immerse the slides in 1XSSC for 5 mins to remove the cover slips.
9. Wash slides in stringency solution (formamide:2XSSC, 50:50) at 45C, 5
mins X2.
10. Wash slides in 1XSSC at 45C, 5minsX2.
11. Wash slides in detergent wash solution at 45C, 5minX1.
12. Rinse slides in PBS.
13. Air dry for a couple of minutes and counterstain with DAPI.
14. Mount the slides and observe the results or keep in 4C for late check.
Are there any problems with this procedure? Please help me to check, or if
you could recommend a working protocol you had used, I would really
appreciate.
Thanks,
Jinhui
------------------------------
Message: 15
Date: Wed, 10 Mar 2010 08:06:57 -0500
From: "Dana Settembre" <settembr <@t> umdnj.edu>
Subject: Re: [Histonet] p16
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>, "Deanne Knutson"
<DKnutson <@t> primecare.org>
Message-ID: <sb975329.087 <@t> smtpnpc.umdnj.edu>
Content-Type: text/plain; charset=US-ASCII
Hi Deanne,
My understanding is that you must get p16 from MTM Laboratories. They
have some sort of monopoly right now. I still have some leftover from
Cell Marque and when I run low or the antibody is about to expire I must
also go to MTM.
I currently use the Cell Marque p16 Ready To Use by pretreating with
Dako's TRS for 40min in steamer, and detect with labelled polymer.
Dako's Envision + Mouse kit. It works nicely.
Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ USA
>>> "Knutson, Deanne" <DKnutson <@t> primecare.org> 03/09/10 5:27 PM >>>
Hello fellow Histonetters,
Does anyone run the antibody p16? If so, where do you purchase it from
and maybe you can share your protocol with me? Thank you in advance.
Deanne Knutson
Anatomic Pathology Supervisor
St. Alexius Medical Center
(701)-530-6730
dknutson <@t> primecare.org<mailto:dknutson <@t> primecare.org>
________________________________
This email may include confidential and privileged information. If this
is not intended for your use, please destroy immediately and contact the
sender of the message.
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 16
Date: Wed, 10 Mar 2010 08:25:25 -0500
From: "Cynthia Pyse" <cpyse <@t> x-celllab.com>
Subject: RE: [Histonet] p16
To: "'Knutson, Deanne'" <DKnutson <@t> primecare.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001cac055$24c65010$6e52f030$@com>
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We purchase our p16 through MTM laboratories reference # 9517.
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cpyse <@t> x-celllab.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Knutson,
Deanne
Sent: Tuesday, March 09, 2010 5:28 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] p16
Hello fellow Histonetters,
Does anyone run the antibody p16? If so, where do you purchase it from and
maybe you can share your protocol with me? Thank you in advance.
Deanne Knutson
Anatomic Pathology Supervisor
St. Alexius Medical Center
(701)-530-6730
dknutson <@t> primecare.org<mailto:dknutson <@t> primecare.org>
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------------------------------
Message: 17
Date: Wed, 10 Mar 2010 09:15:19 -0500
From: "Durden, Kelley" <kelleydurden <@t> pathology.ufl.edu>
Subject: [Histonet] Masson Trichrome, Alligator Heart Tissue, colors
turning out purple
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<92E6B93E0A3D544C87DDDE33E7608AAE591DBE3A <@t> HSC-CMS01.ad.ufl.edu>
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I've been using the Masson Trichrome kit from Richard Allan since 2001 and
I've always gotten really great results. It is a little more expensive than
using home made reagents but the results are very consistent.
Kelley
------------------------------
Message: 18
Date: Wed, 10 Mar 2010 06:44:18 -0800
From: Green JumpyOne <greenjumpyone <@t> hotmail.com>
Subject: [Histonet] Microwave Tissue Processor Protocols
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY107-W37DD5A5D44A2566D4154F5AB330 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
I am working on the validation of our microwave processors and have noticed
that the tissues are a bit dry. Would anyone be willing to share their
protocol timings for their fixation, ethanol, isopropyl and wax impregnation
steps?
We are using the Histos-5 microwaves from Milestone and are utilizing the
2mm (for the smalls) and the 3mm (for the larger specimens) default program
settings.
thanks for any help!
Michelle
ps, my apologies to the board for someone spamming the board using a spoof
of my email address. :o(
_________________________________________________________________
Your E-mail and More On-the-Go. Get Windows Live Hotmail Free.
http://clk.atdmt.com/GBL/go/201469229/direct/01/
------------------------------
Message: 19
Date: Wed, 10 Mar 2010 08:54:11 -0600
From: "Amy Johnson" <AJohnson <@t> aipathology.com>
Subject: [Histonet] HistoDay
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<704247D5A09D004C9E6B115138D1703A1C4B89 <@t> hpserv001.aipathology.local>
Content-Type: text/plain; charset="us-ascii"
Happy Histology Professionals Day!!!!!!
When I was going to school at Marshfield one of the Pathologists told me
that without histotechs his work would be much harder.
Thank you Dr. Krawicz for appreciating the job we do!!!
------------------------------
Message: 20
Date: Wed, 10 Mar 2010 08:02:17 -0700
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Histology Professionals Day
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4D14F0FC9316DD41972D5F03C070908B02E46F26 <@t> nmdamailsvr.nmda.ad.nmsu.edu>
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If it weren't for a med-tech-turned-pathologist that took me up on my
interest in becoming trained in histology (1967), I would not have just
marked my 41st year as a histologist. Back then, you just had to have
OJT and study independently for your written and practical tests. I had
no idea what I was getting into but it was one of the absolute best
choices I ever made. To pretend to quote some baseball player,
"Histology been berry, berry good to me"!
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
------------------------------
Message: 21
Date: Wed, 10 Mar 2010 09:07:17 -0600
From: "Nita Searcy" <NSEARCY <@t> swmail.sw.org>
Subject: Re: [Histonet] Histology Professionals Day
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>, "Sara Breeden"
<sbreeden <@t> nmda.nmsu.edu>
Message-ID: <4B976145.5D38.00EF.0 <@t> swmail.sw.org>
Content-Type: text/plain; charset="us-ascii"
I too "stumbled" into the profession in 1967 and share Sara's sentiments.
I sent the following to our senior staff as well as the supervisory group of
our laboratory:
Today is the first annual National Histotechnology day and is being
celebrated by the histology staff by a morning breakfast ( in order that the
entire staff can participate) and the wearing of special t-shirts.
In spite of almost total anonymity to most of the general public, as well as
a considerable amount of health care professionals, these highly skilled
individuals play a vital role in healthcare.
Wish them congratulations.
Nita Searcy, HT/HTL (ASCP)
Scott and White Hospital
Division Manager, Anatomic Pathology
2401 S. 31st. Street
254-724-2438
Temple, Texas, 76502
nsearcy <@t> swmail.sw.org
254-724-2438
>>> "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu> 3/10/2010 9:02 AM >>>
If it weren't for a med-tech-turned-pathologist that took me up on my
interest in becoming trained in histology (1967), I would not have just
marked my 41st year as a histologist. Back then, you just had to have
OJT and study independently for your written and practical tests. I had
no idea what I was getting into but it was one of the absolute best
choices I ever made. To pretend to quote some baseball player,
"Histology been berry, berry good to me"!
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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BEGIN:VCARD
VERSION:2.1
X-GWTYPE:USER
FN:Nita Searcy
TEL;WORK:4-2438
ORG:;Anatomic Pathology
EMAIL;WORK;PREF;NGW:NSEARCY <@t> swmail.sw.org
N:Searcy;Nita
TITLE:Manager, Pathology Division
TEL;PAGER:633-2370
END:VCARD
BEGIN:VCARD
VERSION:2.1
X-GWTYPE:USER
FN:Nita Searcy
TEL;WORK:4-2438
ORG:;Anatomic Pathology
EMAIL;WORK;PREF;NGW:NSEARCY <@t> swmail.sw.org
N:Searcy;Nita
TITLE:Manager, Pathology Division
TEL;PAGER:633-2370
END:VCARD
------------------------------
Message: 22
Date: Wed, 10 Mar 2010 10:09:18 -0500
From: Alyssa Peterson <alyssa <@t> alliedsearchpartners.com>
Subject: [Histonet] Happy HPD to our "Unsung Heroes"
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<bbc6db3a1003100709i53003bf5q8e2eb2fc73ef2336 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Allied Search Partners wants to wish you a Happy Histology Professionals
Day!
Histology professionals are the unsung heroes behind the laboratory doors.
The patient may not know you but what you all do is the absolute best for
them because what you do affects their lives and their loved ones lives.
I want to thank each and every one of you for doing what you do behind
closed doors away from the surgeons who see the patients and actually get to
remove the problem.
Thank you for being the Unspoken Heroes!!
Please contact us today to inquire about Histology jobs in your area.
Alyssa Peterson
Director of Recruitment
Allied Search Partners
Alyssa <@t> alliedsearchpartners.com
WWW.ALLIEDSEARCHPARTNERS.COM <http://www.alliedsearchpartners.com/>
------------------------------
Message: 23
Date: Wed, 10 Mar 2010 10:13:35 -0500
From: Merced M Leiker <leiker <@t> buffalo.edu>
Subject: Re: [Histonet] Need help with FISH staining protocol on
frozen tissue sections
To: "Shen, Jinhui" <jinhui <@t> uta.edu>, histonet <@t> lists.utsouthwestern.edu
Message-ID: <CFAEA1255B1583D39CF3E2F4 <@t> CDYwxp1931.ad.med.buffalo.edu>
Content-Type: text/plain; charset=us-ascii; format=flowed
I have been using IDLabs porcine Y chromosome green label probe on frozen
pig tissue with good results most of the time. You say you denature the
probe and then hold it at 37oC...I would think that would allow the probe
to reanneal to itself since you also hybridize later on at that temp...try
following the IDLabs protocol where they say to co-denature the probe and
the slide. I do this for my frozen sections and it works.
Regards,
Merced
--On Wednesday, March 10, 2010 12:44 AM -0600 "Shen, Jinhui"
<jinhui <@t> uta.edu> wrote:
> Hi everyone,
>
> Has anyone done FISH staining on mice tissue frozen sections for Y
> chromosome? I need help with a successful protocol.
>
> Recently I started to do FISH staining on mice liver frozen sections for
> chromosome Y, but got no positive results even on male samples yet. The
> probe I used was IDMF1057 Green label Chr Y probe from ID Labs Inc.
> (www.idlabs.com). They have a protocol online probably for cultured cells
> (http://www.idlabs.com/product/datasheets/IDMF1057.pdf?PHPSESSID=b39f1650
> 7cf76b69495166c651f0b52c) which I haven't tried yet. I followed the
> following protocol for frozen sections:
>
> 1. Fix sections in 4% PFA at 4C for 10 mins, then wash in PBS.
>
> 2. Treat the sections with 0.01% Pepsin, 37C 10min, wash in PBS.
>
> 3. Dehydrate the slides using 70% Ethanol (2minsX2), 90% Ethanol (2mins
> X2), 100% Ethanol (5 mins X1).Then Age the slides at 65C for one hour.
>
> 4. Mix the probe and hybridization buffer. Denature the probe by incubate
> it at 65C for 10 mins, then hold it at 37C for 30 - 60 mins.
>
> 5. Denature the sections by incubate them in denaturing solution
> (formamide:2XSSC, 70:30) at 75C for 2 mins.
>
> 6. Quench the slides in 4C 70% Ethanol for 4 mins. Dehydrate the slides
> as step 3.
>
> 7. Apply the probe and hybridize at 37C overnight.
>
> 8. Immerse the slides in 1XSSC for 5 mins to remove the cover slips.
>
> 9. Wash slides in stringency solution (formamide:2XSSC, 50:50) at 45C, 5
> mins X2.
>
> 10. Wash slides in 1XSSC at 45C, 5minsX2.
>
> 11. Wash slides in detergent wash solution at 45C, 5minX1.
>
> 12. Rinse slides in PBS.
>
> 13. Air dry for a couple of minutes and counterstain with DAPI.
>
> 14. Mount the slides and observe the results or keep in 4C for late check.
>
> Are there any problems with this procedure? Please help me to check, or
> if you could recommend a working protocol you had used, I would really
> appreciate.
>
> Thanks,
>
> Jinhui
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
------------------------------
Message: 24
Date: Wed, 10 Mar 2010 09:13:46 -0600
From: "Beckham, Sharon" <SLB <@t> stowers.org>
Subject: [Histonet] RE: Histology Professionals Day
To: "'Breeden, Sara'" <sbreeden <@t> nmda.nmsu.edu>, histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BD62CBAC4395B94096109020651BE2EC12FF1219D0 <@t> EXCHMB-02.stowers-institute.org>
Content-Type: text/plain; charset="us-ascii"
Sally, I have pretty much the same story. Back in 1970 I applied for a
pathology secretary position and the pathologist asked me if I could sew,
which meant he wondered if I could do intricate work with my hands. In my
spare time, I trained in histology and took my registry in 1973. It was the
best decision I ever made to take him up on his offer. I love, love, love
being a histologist and hope that my health remains good so I can do it for
many more years to come.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Wednesday, March 10, 2010 9:02 AM
To: histonet
Subject: [Histonet] Histology Professionals Day
If it weren't for a med-tech-turned-pathologist that took me up on my
interest in becoming trained in histology (1967), I would not have just
marked my 41st year as a histologist. Back then, you just had to have OJT
and study independently for your written and practical tests. I had no idea
what I was getting into but it was one of the absolute best choices I ever
made. To pretend to quote some baseball player, "Histology been berry,
berry good to me"!
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 25
Date: Wed, 10 Mar 2010 09:13:45 -0600
From: "Marsh, Nannette" <NMP <@t> stowers.org>
Subject: [Histonet] RE: HistoDay
To: 'Amy Johnson' <AJohnson <@t> aipathology.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BD62CBAC4395B94096109020651BE2EC12FEFE2C7A <@t> EXCHMB-02.stowers-institute.org>
Content-Type: text/plain; charset="us-ascii"
Here's a quote that we have posted on our Histology event board "Ah, the
Professional Hostologist! Walk in awe when you meet one....because there
are only a few people who can produce something as beautiful and useful as a
slide." by Drs. William Osler and F.E. Mohs----just about says it all,
doesn't it?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amy Johnson
Sent: Wednesday, March 10, 2010 8:54 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] HistoDay
Happy Histology Professionals Day!!!!!!
When I was going to school at Marshfield one of the Pathologists told me
that without histotechs his work would be much harder.
Thank you Dr. Krawicz for appreciating the job we do!!!
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 26
Date: Wed, 10 Mar 2010 09:18:54 -0600
From: "Marsh, Nannette" <NMP <@t> stowers.org>
Subject: [Histonet] RE: HistoDay
To: 'Amy Johnson' <AJohnson <@t> aipathology.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BD62CBAC4395B94096109020651BE2EC12FEFE2C7B <@t> EXCHMB-02.stowers-institute.org>
Content-Type: text/plain; charset="us-ascii"
Okay--so I don't proof read very well--that was supposed to be
'histologist'--not hostologist in my last post--although there are days when
hostility abounds in clinical pathology!!!!!
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amy Johnson
Sent: Wednesday, March 10, 2010 8:54 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] HistoDay
Happy Histology Professionals Day!!!!!!
When I was going to school at Marshfield one of the Pathologists told me
that without histotechs his work would be much harder.
Thank you Dr. Krawicz for appreciating the job we do!!!
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 27
Date: Wed, 10 Mar 2010 08:21:42 -0700
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Histo Professionals Day
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4D14F0FC9316DD41972D5F03C070908B02E46F28 <@t> nmdamailsvr.nmda.ad.nmsu.edu>
Content-Type: text/plain; charset="US-ASCII"
Perhaps what each of us ought to do is write a quick line naming the one
person that got you involved in histology and how they did that. I'd be
willing to gather them and pass them on to NSH so they could post it at
the Seattle meeting. If you'd like to do this, send the email to
nmhisto <@t> comcast.net and I'll collect. Just an idea...
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
------------------------------
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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