[Histonet] Helicobacter Pylori antibody

[Cynthia Pyse]

We purchase it from Biocare. Protocol: pretreatment Diva (Biocare) 20
minutes water bath 20 minutes cool down. Detection system Mach 4 (Biocare)
10 minutes H2O2, 10 minute primary antibody dilution 1:100, 5 minutes mouse
probe, 12 minutes polymer, 10 minutes DAB(Dako), 5 minutes
hematoxylin(Dako).  
Works great.
Cindy
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Chakib
Boussahmain
Sent: Tuesday, March 09, 2010 1:46 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Helicobacter Pylori antibody

Hey Histonet,
Is anyone using Helicobacter pylori antibody? if so, can you tell me where
did you get it? and the protocol?
Any input will appreciated
Thank you so much.
Chakib HTL(ASCP)
MIT-DCM


      
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet