[Histonet] Helicobacter Pylori antibody

[Sebree Linda A]

Hi Chakib,

We use Ventana's (manufactured by Cell Marque) H. Pylori on their instruments.  The protocol is specific to the instruments but involves a routine HIER step and Avidin/Biotin blocking.  The antibody itself is ready-to-use but is available from Cell Marque as a concentrate as well.  The protocol produces a very clean stain; essential since these bugs are so small.

Hope this helps,

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Chakib Boussahmain
Sent: Tuesday, March 09, 2010 12:46 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Helicobacter Pylori antibody


Hey Histonet,
Is anyone using Helicobacter pylori antibody? if so, can you tell me where did you get it? and the protocol? Any input will appreciated Thank you so much. Chakib HTL(ASCP) MIT-DCM


      
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