[Histonet] Negative controls

Houston, Ronald Ronald.Houston <@t> nationwidechildrens.org
Thu Mar 4 09:52:28 CST 2010

We too use the Universal negative from Biocare, and it has passed
through 2 CAP inspections so no problem on that front.

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gagnon,
Sent: Thursday, March 04, 2010 10:48 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Negative controls

Hi Anita,
We are currently examining the negative control issue in our laboratory.
We also have Ventanas. We currently use a normal goat serum as our
"non-immune" serum.  We are considering changing our SOP's to use the
Ventana Neg Control Rabbit and Neg Control Mouse in dispensers.  
Linda's suggestion of Biocare's Universal Negative Control Serum is
intriguing.  It would seem regressive to go back to the days of
delineating mouse/rabbit antisera, using separate dispensers for each,
and a universal negative seems to be the way to go, especially when
using universal DAB kits.  Using such a reagent would mean it could be
applied to each case's negative control slide.
Linda or others, would such a negative control serum protocol include
your most aggressive pretreatment? 
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital
Kingston, Ontario, Canada

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