[Histonet] Negative controls
Ronald.Houston <@t> nationwidechildrens.org
Thu Mar 4 09:52:28 CST 2010
We too use the Universal negative from Biocare, and it has passed
through 2 CAP inspections so no problem on that front.
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gagnon,
Sent: Thursday, March 04, 2010 10:48 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Negative controls
We are currently examining the negative control issue in our laboratory.
We also have Ventanas. We currently use a normal goat serum as our
"non-immune" serum. We are considering changing our SOP's to use the
Ventana Neg Control Rabbit and Neg Control Mouse in dispensers.
Linda's suggestion of Biocare's Universal Negative Control Serum is
intriguing. It would seem regressive to go back to the days of
delineating mouse/rabbit antisera, using separate dispensers for each,
and a universal negative seems to be the way to go, especially when
using universal DAB kits. Using such a reagent would mean it could be
applied to each case's negative control slide.
Linda or others, would such a negative control serum protocol include
your most aggressive pretreatment?
Eric Gagnon MLT
Kingston General Hospital
Kingston, Ontario, Canada
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