AW: [Histonet] Re: paraffin processing times for brain

[Gudrun Lang]

Stephen,
we doubled the routine protocol for braintissue from 13 to 24 hour. We
stayed at one hour for the formalin steps, because the tissue is fixed for
several days before processing. This protocol is also used for prostate big
cassettes.
Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Stephen
Kum Jew
Gesendet: Mittwoch, 03. März 2010 21:43
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Re: paraffin processing times for brain

Hi all

    I was wondering if anyone can send me please the optimal paraffin
processing times for brain autopsy tissue.

    Currently the blocks have cut and stained well but 4-5mm thick tissue
has expanded (bulged out) following cutting as if they had absorbed water.

    Solutions been all changed.

    thanks
    Stephen


|Stephen Kum Jew
|Senior Technical Officer
|Discipline of Pathology
|School of Medical Sciences
|Blackburn Building D06
|University of Sydney NSW 2006
|Australia
|Ph: + 61 2 9036 9027
|Fax:+61 2 9351 3429


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet