[Histonet] RE: paraffin processing times for brain

[McMahon, Loralee A]

we used to do one hour in each step formalin through to paraffin (2 steps of paraffin).  
But 4 to 5mm is a little on the thick side.  We used to try for 3 mm.  But didn't always get it that way.

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Stephen Kum Jew [stevenk <@t> med.usyd.edu.au]
Sent: Wednesday, March 03, 2010 3:42 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: paraffin processing times for brain

Hi all

    I was wondering if anyone can send me please the optimal paraffin
processing times for brain autopsy tissue.

    Currently the blocks have cut and stained well but 4-5mm thick tissue
has expanded (bulged out) following cutting as if they had absorbed water.

    Solutions been all changed.

    thanks
    Stephen


|Stephen Kum Jew
|Senior Technical Officer
|Discipline of Pathology
|School of Medical Sciences
|Blackburn Building D06
|University of Sydney NSW 2006
|Australia
|Ph: + 61 2 9036 9027
|Fax:+61 2 9351 3429


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