[Histonet] RE: paraffin processing times for brain

McMahon, Loralee A Loralee_Mcmahon <@t> URMC.Rochester.edu
Wed Mar 3 14:52:34 CST 2010

we used to do one hour in each step formalin through to paraffin (2 steps of paraffin).  
But 4 to 5mm is a little on the thick side.  We used to try for 3 mm.  But didn't always get it that way.

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Stephen Kum Jew [stevenk <@t> med.usyd.edu.au]
Sent: Wednesday, March 03, 2010 3:42 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: paraffin processing times for brain

Hi all

    I was wondering if anyone can send me please the optimal paraffin
processing times for brain autopsy tissue.

    Currently the blocks have cut and stained well but 4-5mm thick tissue
has expanded (bulged out) following cutting as if they had absorbed water.

    Solutions been all changed.


|Stephen Kum Jew
|Senior Technical Officer
|Discipline of Pathology
|School of Medical Sciences
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