[Histonet] Deleting extraneous parts of posts when replying

Merced M Leiker leiker <@t> buffalo.edu
Wed Mar 3 08:25:40 CST 2010

My brief experience with paraffin and fluorescence (DAPI and EGFP) is that 
the paraffin embedding process destroys it.


--On Tuesday, March 02, 2010 3:25 PM -0600 Fabrice gankam 
<gankam <@t> googlemail.com> wrote:

> Hi,
> Just wondering if anyone has use hydroethinide to detect the  free
> radicals (ROS) in the CNS or anyother tissue of rats after 4% formallin
> fixation and parrafin embedding.
> I wanted to used the hydroethidine on paraffin sections.
> I wonder if the fluorescence is lost by fixation (12hrs in 4% PAF) and
> paraffin embedding plus deparaffination.
> All the papers I reviewed used hydroethidine on frozen section but our
> facility does not have vibratome or cryostat.
> Please help
> Please Help.
> Fabrice
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

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