[Histonet] ROS

[John Kiernan]

Reactive oxygen species (ROS) are unstable ions or free radicals including superoxide, peroxynitrite and nitric oxide. Most exist for milliseconds before combining with other substances. Nitric oxide is exceptional because it can move about through cytoplasm, cell membranes and extracellular space for several tenths of a second before being changed into much more short-lived ROS. The fluorescent methods for ROS are for instant photography of cell cultures. 

John Kiernan
Anatomy, UWO
London, Canada 
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----- Original Message -----
From: Fabrice gankam 
Date: Tuesday, March 2, 2010 16:26
Subject: Re: [Histonet] Deleting extraneous parts of posts when replying
To: histonet <@t> lists.utsouthwestern.edu

> Hi,
> Just wondering if anyone has use hydroethinide to detect 
> the free radicals
> (ROS) in the CNS or anyother tissue of rats after 4% formallin 
> fixation and
> parrafin embedding.
> I wanted to used the hydroethidine on paraffin sections.
> I wonder if the fluorescence is lost by fixation (12hrs in 4% 
> PAF) and
> paraffin embedding plus deparaffination.
> All the papers I reviewed used hydroethidine on frozen section 
> but our
> facility does not have vibratome or cryostat.
> Please help
> Please Help.
> 
> Fabrice
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