[Histonet] bone marrow fixative

Rene J Buesa rjbuesa <@t> yahoo.com
Tue Mar 2 14:38:23 CST 2010

Buffered 10% NBF, but with the pH exactly at 7.0, checked for each case. IHC is perfect.
René J.

--- On Tue, 3/2/10, Amy Farnan <Farnana <@t> nehealth.com> wrote:

From: Amy Farnan <Farnana <@t> nehealth.com>
Subject: [Histonet] bone marrow fixative
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, March 2, 2010, 2:12 PM

Good afternoon everyone,

In past years our lab used B-5 fixative on bone marrow biopsies for better immunostaining.
With the chemical hazards of B-5 we switched to AZF fixative and have been using this for the past few years. I am just curious what everyone else is using for fixative on their bone marrows and how is your immunoreactivity?  Maybe a better question is what is the preferred fixative for bone marrows?

Have a nice day,
Amy Farnan
Histology Supervisor
Northeast Health

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