FW: [Histonet] Microwave processing and IHCs

[Vickroy, Jim]

See below

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046


-----Original Message-----
From: Vickroy, Jim
Sent: Tuesday, March 02, 2010 8:16 AM
To: 'Mike Pence'
Subject: RE: [Histonet] Microwave processing and IHCs

I understand and agree in principle.  However let me ask this:
If a validation study shows that there is no difference between conventional tissue processing and microwave tissue processing in your IHC protocols then why can't you then use controls that are processed either by conventional or microwave.   If we can't then are we using controls that have been decaled for tissues that have to be decaled and so forth.  I have always believed if you can validate there is no appreciable difference in methods than we can use the same controls.

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Tuesday, March 02, 2010 8:04 AM
To: Green JumpyOne; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Microwave processing and IHCs

Your control tissue needs to be processed the same as your patient
samples.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Green
JumpyOne
Sent: Monday, March 01, 2010 7:15 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Microwave processing and IHCs



Does anyone here have any experience with using microwave tissue
processing and then performing IHC stains on those same tissues?

We have new microwave processors and are trying to determine if we will
need to get all new controls for our IHCs that have been microwave
processed.  If yes, we will need to do validations on the controls, but
I am wondering if the MW will have any discernible effect on the
reactivity of the tissue.

thanks for any help!

Michelle

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