[Histonet] Microwave processing and IHCs

[McMahon, Loralee A]

In my experience I have not seen any change in the MW tissue versus the routine processing.  What we have tried to do since we have so many different antibodies and control tissues is create mini sausage blocks with one piece of MW tissue and one piece of non MW tissue.  Because we were not always sure what tissue were microwaved and what tissues were not.   
For example one small piece of tonsil microwaved and one non microwaved embedded in the same block. That way no matter what your patient tissue sample has gone through you have all the bases covered.  
It takes a long time to set up but will save you time in the end.  
If you run your tissues when you validate the microwave you'll have half of the process finished. 

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mike Pence [mpence <@t> grhs.net]
Sent: Tuesday, March 02, 2010 9:03 AM
To: Green JumpyOne; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Microwave processing and IHCs

Your control tissue needs to be processed the same as your patient
samples.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Green
JumpyOne
Sent: Monday, March 01, 2010 7:15 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Microwave processing and IHCs



Does anyone here have any experience with using microwave tissue
processing and then performing IHC stains on those same tissues?

We have new microwave processors and are trying to determine if we will
need to get all new controls for our IHCs that have been microwave
processed.  If yes, we will need to do validations on the controls, but
I am wondering if the MW will have any discernible effect on the
reactivity of the tissue.

thanks for any help!

Michelle

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