[Histonet] H. pylori RTU antibody

Mark Tarango marktarango <@t> gmail.com
Wed Jun 30 11:13:40 CDT 2010


Biocare has a rabbit polyclonal anti-HP ab and a mouse monoclonal.  Which
one do you use?

Mark
On Wed, Jun 30, 2010 at 8:05 AM, McMahon, Loralee A <
Loralee_Mcmahon <@t> urmc.rochester.edu> wrote:

> Use the RTU from Biocare.  It is clean and easy.  You can use it with the
> Dako Flex Kit.
>
> Loralee McMahon, HTL (ASCP)
> Immunohistochemistry Supervisor
> Strong Memorial Hospital
> Department of Surgical Pathology
> (585) 275-7210
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Drew Meyer [
> 41dmb41 <@t> gmail.com]
> Sent: Wednesday, June 30, 2010 10:41 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] H. pylori RTU antibody
>
> I was just wondering if a lot of people use the RTU antibodies available
> out
> there for H. pylori.  Currently, we're testing the RTU from Dako and we're
> having issues with a lot of background staining.  After tweaking the
> protocol a lot, I've managed to get a lot of it removed, but there are
> still
> some issues.  I was wondering if most people are making their own dilutions
> to get a better stain or if the RTUs are commonplace.  I appreciate the
> feedback!
>
> Thanks,
> Drew
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