[Histonet] rehydrating dried out tissue and gram positive control(forget the slim jim)

Madary, Joseph MadaryJ <@t> MedImmune.com
Wed Jun 23 13:12:34 CDT 2010


I worked at AFIP for 16 years and worked in infectious disease for 6 and
we never used a slim jim.  People discussed it but the truth is there
was literally thousands of gram positive samples to use, why would we
have wanted to use a slim jim? Use appendix that has been tested.  

 

Dried out tissue as I recall an overnight soak in some 15ml glycerin, 65
ML NBFformalin and 20 ml Absolute alcohol and then wash in NBF a few
times before the processor.  Might work.

 

Nick Madary, HT/HTL(ASCP)QIHC

Medimmune Histology Mgr, 

OMW, Area 4, Lab 2438

301.398.4745(vm)

301.398.6360(lab)

301.398.9745(fax)

 




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