[Histonet] RE:Empty specimen containers

Goodwin, Diana Goodwd2 <@t> LabCorp.com
Wed Jun 23 10:47:39 CDT 2010


We retain our empty containers for 2 weeks post-final report.

D. Goodwin
SJGI
Marlton, NJ

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu [histonet-request <@t> lists.utsouthwestern.edu]
Sent: Wednesday, June 23, 2010 9:02 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 79, Issue 28

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Today's Topics:

   1. RE: Breezy lab/flyaway ribbons! (R J VAZQUEZ)
   2. slim jims (Perry, Margaret)
   3. RE: Breezy lab/flyaway ribbons! (Gill, Caula A.)
   4. Re: Autofluorescence in murine white adipose tissue
      cryosections (Johnson, Teri)
   5. Re: slim jims (V. Neubert)
   6. X-gal staining troubleshooting (Johnson, Teri)
   7. Glut1 antibody (Martha Ward)
   8. RE: Breezy lab/flyaway ribbons! (Sebree Linda A)
   9. Re: slim jims (Emily Sours)
  10. IHC for Legionella? (Harrison, Sandra C.)
  11. (no subject) (Hartz, Rhonda  SktnHR)
  12. Re: IHC for Legionella? (Richard Cartun)
  13. Re: Help! In need of positive Gram Control (Jay Lundgren)
  14. Re: (no subject) (Jennifer MacDonald)
  15. Oven for paraffin slide drying (Mia Woodruff)
  16. Re: Help! In need of positive Gram Control (Jackie M
      O'Connor) (Robert Richmond)
  17. Re: Oven for paraffin slide drying (Emily Sours)
  18. Re: Re: Amyloidosis control tissue (Komal Gada)
  19. RE: Help! In need of positive Gram Control (connie grubaugh)
  20. RE: New CAP question ANP.22760  (JMyers1 <@t> aol.com)
  21. RE: Help! In need of positive Gram Control
      (Susan.Walzer <@t> HCAHealthcare.com)
  22. RE: Glut1 antibody (Hoekert, W.E.J.)
  23. Retaining empty jars (mtitford <@t> aol.com)


----------------------------------------------------------------------

Message: 1
Date: Tue, 22 Jun 2010 11:07:32 -0700
From: R J VAZQUEZ <sweething63 <@t> msn.com>
Subject: RE: [Histonet] Breezy lab/flyaway ribbons!
To: <histotech <@t> imagesbyhopper.com>,
        <trathborne <@t> somerset-healthcare.com>,   <jcbritton <@t> cheshire-med.com>,
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT121-W529A2CE59D5619E7BFCC8CB8C40 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"


When I was doing histology, I would use a flexible end of a brush to roll the ribbon end closest to the blade and that would secure it beautifully and no fly aways.

Robyn Vazquez

> From: histotech <@t> imagesbyhopper.com
> To: trathborne <@t> somerset-healthcare.com; JCBRITTON <@t> Cheshire-Med.COM; histonet <@t> lists.utsouthwestern.edu
> Date: Tue, 22 Jun 2010 11:10:29 -0400
> Subject: RE: [Histonet] Breezy lab/flyaway ribbons!
> CC:
>
> We have a similar situation, but I have shown my techs how to 1) wet the
> brush they use to take the ribbon off the knife and 2) to roll/curl the
> ribbon over the damp end of the brush and 3) hover our hand over the top of
> the ribbon while moving it (to reduce the breeze) from the microtome to the
> waterbath.
>
> I learned this technique when I first started in Histology, as we had a
> choice, a HOT room with no AC and no wind, or learn how to deal with the
> breeze and don't sweat! I opted for dealing with the wind. Now, it's
> second nature and it matters not whether there is wind or not, I still use
> the same motion for tranfer of the ribbon!
>
> Good luck with your situation. :o)
>
> Michelle
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rathborne,
> Toni
> Sent: Tuesday, June 22, 2010 8:50 AM
> To: Josie Britton; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Breezy lab/flyaway ribbons!
>
>
> For the ceiling vents, you could ask to have deflectors installed. The ones
> we have are made of stainless, are slightly larger than the vent, and are
> suspended about 6"-8" below the opening.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Josie Britton
> Sent: Tuesday, June 22, 2010 6:23 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Breezy lab/flyaway ribbons!
>
>
> We have been having trouble with big breeze blowing our precious ribbons
>
> out of our hands while cutting. We would like a door on the Histology
>
> lab to cut down on the breeze of people walking by through the hall.
>
> Our facilities want to find out what other people are doing to stop this
>
> problem. We also have air ducts blowing down from above, which is not
>
> helping the problem. We would like as many labs solutions as possible.
>
> Our facilities have come up with all these crazy barriers that we would
>
> have to move to walk around when we need to put our racks on the
>
> stainer, answer timers, print more slides, use the oven, etc...
>
>
>
>
>
>
>
> Any input would be appreciated!
>
>
>
>
>
>
>
> Breezy girls,
>
>
>
>
>
>
>
> Josie Britton and Chris Braaten
>
>
>
> Cheshire Medical Center
>
>
>
> Keene, NH 03431
>
>
>
>
> CONFIDENTIALITY NOTICE: This electronic message, including any attachments,
> is for the sole use of the intended recipients and may contain confidential
> and privileged information. Any unauthorized review, use, disclosure or
> distribution is prohibited. If you are not the intended recipient, please
> contact the sender by electronic mail and destroy all copies of the original
> message. _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> CONFIDENTIALITY NOTICE
> This message and any included attachments are from Somerset Medical Center
> and are intended only for the addressee. The information contained in this
> message is confidential and may contain privileged, confidential,
> proprietary and/or trade secret information entitled to protection and/or
> exemption from disclosure under applicable law. Unauthorized forwarding,
> printing, copying, distribution, or use of such information is strictly
> prohibited and may be unlawful. If you are not the addressee, please
> promptly delete this message and notify the sender of the delivery error by
> e-mail or you may call Somerset Medical Center's computer Help Desk at
> 908-685-2200, ext. 4050.
>
> Be sure to visit Somerset Medical Center's Web site -
> www.somersetmedicalcenter.com - for the most up-to-date news,
> event listings, health information and more.
> No virus found in this incoming message.
> Checked by AVG - www.avg.com
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> 06:36:00
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>
>
>
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 2
Date: Tue, 22 Jun 2010 13:47:33 -0500
From: "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
Subject: [Histonet] slim jims
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <FCA5EF47F9BC694CBB4C58FEA04219634DBDB8D7BC <@t> SDSU-MBX.jacks.local>
Content-Type: text/plain; charset="us-ascii"

Do you put the slim jims in formalin and then process them or just put them in the processor?
Margaret


------------------------------

Message: 3
Date: Tue, 22 Jun 2010 15:41:41 -0400
From: "Gill, Caula A." <cgill <@t> marylandgeneral.org>
Subject: RE: [Histonet] Breezy lab/flyaway ribbons!
To: "R J VAZQUEZ" <sweething63 <@t> msn.com>,
        <histotech <@t> imagesbyhopper.com>, <trathborne <@t> somerset-healthcare.com>,
        <jcbritton <@t> cheshire-med.com>,   <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <087A9911BBAFDE4B8151CB148586E2C23A9EAD <@t> MDGEN-EXCH1.marylandgeneral.org>

Content-Type: text/plain;       charset="us-ascii"

Ditto, I use a small brush that I got from the craft store and prior to
picking up the ribbon I wet the brush and slip it under my ribbon end
closest to the blade and presto no flyaways.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of R J
VAZQUEZ
Sent: Tuesday, June 22, 2010 2:08 PM
To: histotech <@t> imagesbyhopper.com; trathborne <@t> somerset-healthcare.com;
jcbritton <@t> cheshire-med.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Breezy lab/flyaway ribbons!


When I was doing histology, I would use a flexible end of a brush to
roll the ribbon end closest to the blade and that would secure it
beautifully and no fly aways.

Robyn Vazquez

> From: histotech <@t> imagesbyhopper.com
> To: trathborne <@t> somerset-healthcare.com; JCBRITTON <@t> Cheshire-Med.COM;
> histonet <@t> lists.utsouthwestern.edu
> Date: Tue, 22 Jun 2010 11:10:29 -0400
> Subject: RE: [Histonet] Breezy lab/flyaway ribbons!
> CC:
>
> We have a similar situation, but I have shown my techs how to 1) wet
> the brush they use to take the ribbon off the knife and 2) to
> roll/curl the ribbon over the damp end of the brush and 3) hover our
> hand over the top of the ribbon while moving it (to reduce the breeze)

> from the microtome to the waterbath.
>
> I learned this technique when I first started in Histology, as we had
> a choice, a HOT room with no AC and no wind, or learn how to deal with

> the breeze and don't sweat! I opted for dealing with the wind. Now,
> it's second nature and it matters not whether there is wind or not, I
> still use the same motion for tranfer of the ribbon!
>
> Good luck with your situation. :o)
>
> Michelle
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Rathborne, Toni
> Sent: Tuesday, June 22, 2010 8:50 AM
> To: Josie Britton; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Breezy lab/flyaway ribbons!
>
>
> For the ceiling vents, you could ask to have deflectors installed. The

> ones we have are made of stainless, are slightly larger than the vent,

> and are suspended about 6"-8" below the opening.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Josie
> Britton
> Sent: Tuesday, June 22, 2010 6:23 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Breezy lab/flyaway ribbons!
>
>
> We have been having trouble with big breeze blowing our precious
> ribbons
>
> out of our hands while cutting. We would like a door on the Histology
>
> lab to cut down on the breeze of people walking by through the hall.
>
> Our facilities want to find out what other people are doing to stop
> this
>
> problem. We also have air ducts blowing down from above, which is not
>
> helping the problem. We would like as many labs solutions as possible.
>
> Our facilities have come up with all these crazy barriers that we
> would
>
> have to move to walk around when we need to put our racks on the
>
> stainer, answer timers, print more slides, use the oven, etc...
>
>
>
>
>
>
>
> Any input would be appreciated!
>
>
>
>
>
>
>
> Breezy girls,
>
>
>
>
>
>
>
> Josie Britton and Chris Braaten
>
>
>
> Cheshire Medical Center
>
>
>
> Keene, NH 03431
>
>
>
>
> CONFIDENTIALITY NOTICE: This electronic message, including any
> attachments, is for the sole use of the intended recipients and may
> contain confidential and privileged information. Any unauthorized
> review, use, disclosure or distribution is prohibited. If you are not
> the intended recipient, please contact the sender by electronic mail
> and destroy all copies of the original message.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> CONFIDENTIALITY NOTICE
> This message and any included attachments are from Somerset Medical
> Center and are intended only for the addressee. The information
> contained in this message is confidential and may contain privileged,
> confidential, proprietary and/or trade secret information entitled to
> protection and/or exemption from disclosure under applicable law.
> Unauthorized forwarding, printing, copying, distribution, or use of
> such information is strictly prohibited and may be unlawful. If you
> are not the addressee, please promptly delete this message and notify
> the sender of the delivery error by e-mail or you may call Somerset
> Medical Center's computer Help Desk at 908-685-2200, ext. 4050.
>
> Be sure to visit Somerset Medical Center's Web site -
> www.somersetmedicalcenter.com - for the most up-to-date news, event
> listings, health information and more.
> No virus found in this incoming message.
> Checked by AVG - www.avg.com
> Version: 8.5.439 / Virus Database: 271.1.1/2950 - Release Date:
> 06/22/10 06:36:00
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 4
Date: Tue, 22 Jun 2010 14:52:37 -0500
From: "Johnson, Teri" <TJJ <@t> stowers.org>
Subject: [Histonet] Re: Autofluorescence in murine white adipose
        tissue  cryosections
To: "'histonet <@t> lists.utsouthwestern.edu'"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <BD62CBAC4395B94096109020651BE2EC130875D0C6 <@t> EXCHMB-02.stowers-institute.org>

Content-Type: text/plain; charset="us-ascii"

Dear Frank,

Good luck with ridding your samples of autofluorescence. Fat is always very brightly fluorescent and I suspect it might even be so without any aldehyde fixation. First I wondered how successful you'd be using Sudan Black B, knowing it is a fat stain, and knowing that it's been published for this purpose. So you might try just that and see how that works. In addition you could try using a combination of UV irradiation and the Sudan Black B and see if the combination works for you. They use paraffin sections in this reference, but it might work as well with cryosections. http://www.microscopyu.com/references/pdfs/Viegas_etal_Eur_J_Histochem-51-59-2007.pdf

Good luck, and do report back if you find a solution to this dilemma!

Best wishes,
Teri Johnson, HT(ASCP)QIHC
Managing Director, Histology Facility
Stowers Institute for Medical Research
Kansas City, MO




------------------------------

Message: 5
Date: Tue, 22 Jun 2010 21:58:39 +0200
From: "V. Neubert" <histonet.nospam <@t> vneubert.com>
Subject: Re: [Histonet] slim jims
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4C2115EF.7030404 <@t> vneubert.com>
Content-Type: text/plain; charset=ISO-8859-1

I just found "a partially eaten Slim Jim snack" picture on wikipedia...

Is it Friday again? :->

> Do you put the slim jims in formalin and then process them or just put them in the processor?
> Margaret
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>




------------------------------

Message: 6
Date: Tue, 22 Jun 2010 15:02:44 -0500
From: "Johnson, Teri" <TJJ <@t> stowers.org>
Subject: [Histonet] X-gal staining troubleshooting
To: "'histonet <@t> lists.utsouthwestern.edu'"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <BD62CBAC4395B94096109020651BE2EC130875D0C7 <@t> EXCHMB-02.stowers-institute.org>

Content-Type: text/plain; charset="us-ascii"

Hi all,

I have a question about whole mount x-gal staining. Do any of you have experience with your samples turning brown after incubation with the x-gal?  If yes, do you know what causes this and how to keep it from happening?

Thanks in advance,

Teri Johnson, HT(ASCP)QIHC
Managing Director, Histology Facility
Stowers Institute for Medical Research
Kansas City, MO




------------------------------

Message: 7
Date: Tue, 22 Jun 2010 16:10:12 -0400
From: "Martha Ward" <mward <@t> wfubmc.edu>
Subject: [Histonet] Glut1 antibody
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <61135F0455D33347B5AAE209B903A30433DEBA4A <@t> EXCHVS2.medctr.ad.wfubmc.edu>

Content-Type: text/plain;       charset="us-ascii"

I have been using anti-human Glut-1 from Dako for several years but when
I went to order it I was told it was discontinued.  Does anyone have a
suggestion of where I can locate this antibody?  Thanks in advance for
your help!

Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
336-716-2104





------------------------------

Message: 8
Date: Tue, 22 Jun 2010 15:28:50 -0500
From: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Subject: RE: [Histonet] Breezy lab/flyaway ribbons!
To: "Gill, Caula A." <cgill <@t> marylandgeneral.org>,       "R J VAZQUEZ"
        <sweething63 <@t> msn.com>, <histotech <@t> imagesbyhopper.com>,
        <trathborne <@t> somerset-healthcare.com>, <jcbritton <@t> cheshire-med.com>,
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <8C023B4AB999614BA4791BAEB26E2738399EDC <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>

Content-Type: text/plain;       charset="us-ascii"

I do that too using either a wet brush or applicator stick but sometimes
the breeze is more like a gale force wind breaking my ribbon apart to
then adhere to everything but the surface of my waterbath!


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gill,
Caula A.
Sent: Tuesday, June 22, 2010 2:42 PM
To: R J VAZQUEZ; histotech <@t> imagesbyhopper.com;
trathborne <@t> somerset-healthcare.com; jcbritton <@t> cheshire-med.com;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Breezy lab/flyaway ribbons!

Ditto, I use a small brush that I got from the craft store and prior to
picking up the ribbon I wet the brush and slip it under my ribbon end
closest to the blade and presto no flyaways.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of R J
VAZQUEZ
Sent: Tuesday, June 22, 2010 2:08 PM
To: histotech <@t> imagesbyhopper.com; trathborne <@t> somerset-healthcare.com;
jcbritton <@t> cheshire-med.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Breezy lab/flyaway ribbons!


When I was doing histology, I would use a flexible end of a brush to
roll the ribbon end closest to the blade and that would secure it
beautifully and no fly aways.

Robyn Vazquez

> From: histotech <@t> imagesbyhopper.com
> To: trathborne <@t> somerset-healthcare.com; JCBRITTON <@t> Cheshire-Med.COM;
> histonet <@t> lists.utsouthwestern.edu
> Date: Tue, 22 Jun 2010 11:10:29 -0400
> Subject: RE: [Histonet] Breezy lab/flyaway ribbons!
> CC:
>
> We have a similar situation, but I have shown my techs how to 1) wet
> the brush they use to take the ribbon off the knife and 2) to
> roll/curl the ribbon over the damp end of the brush and 3) hover our
> hand over the top of the ribbon while moving it (to reduce the breeze)

> from the microtome to the waterbath.
>
> I learned this technique when I first started in Histology, as we had
> a choice, a HOT room with no AC and no wind, or learn how to deal with

> the breeze and don't sweat! I opted for dealing with the wind. Now,
> it's second nature and it matters not whether there is wind or not, I
> still use the same motion for tranfer of the ribbon!
>
> Good luck with your situation. :o)
>
> Michelle
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Rathborne, Toni
> Sent: Tuesday, June 22, 2010 8:50 AM
> To: Josie Britton; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Breezy lab/flyaway ribbons!
>
>
> For the ceiling vents, you could ask to have deflectors installed. The

> ones we have are made of stainless, are slightly larger than the vent,

> and are suspended about 6"-8" below the opening.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Josie
> Britton
> Sent: Tuesday, June 22, 2010 6:23 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Breezy lab/flyaway ribbons!
>
>
> We have been having trouble with big breeze blowing our precious
> ribbons
>
> out of our hands while cutting. We would like a door on the Histology
>
> lab to cut down on the breeze of people walking by through the hall.
>
> Our facilities want to find out what other people are doing to stop
> this
>
> problem. We also have air ducts blowing down from above, which is not
>
> helping the problem. We would like as many labs solutions as possible.
>
> Our facilities have come up with all these crazy barriers that we
> would
>
> have to move to walk around when we need to put our racks on the
>
> stainer, answer timers, print more slides, use the oven, etc...
>
>
>
>
>
>
>
> Any input would be appreciated!
>
>
>
>
>
>
>
> Breezy girls,
>
>
>
>
>
>
>
> Josie Britton and Chris Braaten
>
>
>
> Cheshire Medical Center
>
>
>
> Keene, NH 03431
>
>
>
>
> CONFIDENTIALITY NOTICE: This electronic message, including any
> attachments, is for the sole use of the intended recipients and may
> contain confidential and privileged information. Any unauthorized
> review, use, disclosure or distribution is prohibited. If you are not
> the intended recipient, please contact the sender by electronic mail
> and destroy all copies of the original message.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> CONFIDENTIALITY NOTICE
> This message and any included attachments are from Somerset Medical
> Center and are intended only for the addressee. The information
> contained in this message is confidential and may contain privileged,
> confidential, proprietary and/or trade secret information entitled to
> protection and/or exemption from disclosure under applicable law.
> Unauthorized forwarding, printing, copying, distribution, or use of
> such information is strictly prohibited and may be unlawful. If you
> are not the addressee, please promptly delete this message and notify
> the sender of the delivery error by e-mail or you may call Somerset
> Medical Center's computer Help Desk at 908-685-2200, ext. 4050.
>
> Be sure to visit Somerset Medical Center's Web site -
> www.somersetmedicalcenter.com - for the most up-to-date news, event
> listings, health information and more.
> No virus found in this incoming message.
> Checked by AVG - www.avg.com
> Version: 8.5.439 / Virus Database: 271.1.1/2950 - Release Date:
> 06/22/10 06:36:00
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 9
Date: Tue, 22 Jun 2010 16:50:06 -0400
From: Emily Sours <talulahgosh <@t> gmail.com>
Subject: Re: [Histonet] slim jims
To: "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <AANLkTilNR8o7jxqa1oLRlErhgn2tp_4gvRZfZxODoDNH <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

if by "processor" you mean "my mouth", yes.
the formalin is well, just a formality.
HA!

Emily

Towns are like people. Old ones often have character, the new ones are
interchangeable.
--Wallace Stegner, Angle of Repose


On Tue, Jun 22, 2010 at 2:47 PM, Perry, Margaret <Margaret.Perry <@t> sdstate.edu
> wrote:

> Do you put the slim jims in formalin and then process them or just put them
> in the processor?
> Margaret
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


------------------------------

Message: 10
Date: Tue, 22 Jun 2010 16:21:51 -0500
From: "Harrison, Sandra C." <Sandra.Harrison3 <@t> va.gov>
Subject: [Histonet] IHC for Legionella?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <DB425E28065DA14FAA75A282959620AF043972C5 <@t> VHAV23MSGA2.v23.med.va.gov>
Content-Type: text/plain; charset="us-ascii"

Does anyone know of an IHC antibody for Legionella?



Sandy C. Harrison, HTL (ASCP)

Histology Supervisor

Minneapolis VA

612-467-2449





------------------------------

Message: 11
Date: Tue, 22 Jun 2010 15:55:07 -0600
From: "Hartz, Rhonda  SktnHR" <Rhonda.Hartz <@t> saskatoonhealthregion.ca>
Subject: [Histonet] (no subject)
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B7F15445A710BA4FA7B48F2C55134AD4049286E5 <@t> lou.sktnhr.ca>
Content-Type: text/plain;       charset="us-ascii"

Hi.  This is my first time, so I apologize if I am not clear enough.  We
have had a request from one of our pathologists to retain empty specimen
containers after grossing is complete.  Is anyone aware of any
recommendations, or does anyone out there retain their empty specimen
containers?

Rhonda Hartz
Technologist Supervisor
Anatomic Pathology Division
Saskatoon Health Region
(306) 655-8197
rhonda.hartz <@t> saskatoonhealthregion.ca



------------------------------

Message: 12
Date: Tue, 22 Jun 2010 18:31:49 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: Re: [Histonet] IHC for Legionella?
To: <histonet <@t> lists.utsouthwestern.edu>,        "Sandra C. Harrison"
        <Sandra.Harrison3 <@t> va.gov>
Message-ID: <4C210194.7400.0077.1 <@t> harthosp.org>
Content-Type: text/plain; charset=US-ASCII

I offer IHC for Legionella in my laboratory.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> "Harrison, Sandra C." <Sandra.Harrison3 <@t> va.gov> 6/22/2010 5:21 PM >>>
Does anyone know of an IHC antibody for Legionella?



Sandy C. Harrison, HTL (ASCP)

Histology Supervisor

Minneapolis VA

612-467-2449



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 13
Date: Tue, 22 Jun 2010 17:52:34 -0500
From: Jay Lundgren <jaylundgren <@t> gmail.com>
Subject: Re: [Histonet] Help! In need of positive Gram Control
To: DianaRip1 <@t> aol.com
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <AANLkTim6sJi00siz3dRXPLt6NFLuBJAkZXm9HM0znEcM <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Slim Jim works great.  This is what we used to use at AFIP.  Just slice it
and process it the same as your regular tissue.

                                             Jay A. Lundgren, M.S., HTL
(ASCP)

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 14
Date: Tue, 22 Jun 2010 15:57:21 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: Re: [Histonet] (no subject)
To: "Hartz, Rhonda SktnHR" <Rhonda.Hartz <@t> saskatoonhealthregion.ca>
Cc: histonet <@t> lists.utsouthwestern.edu,
        histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
        <OF57DD29AA.C3C56478-ON8825774A.007E0C27-8825774A.007E20CD <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"

We used to keep them for a week in a bag.  If there were no problems at
the end of the week we disposed of them





"Hartz, Rhonda  SktnHR" <Rhonda.Hartz <@t> saskatoonhealthregion.ca>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
06/22/2010 03:24 PM

To
<histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] (no subject)






Hi.  This is my first time, so I apologize if I am not clear enough.  We
have had a request from one of our pathologists to retain empty specimen
containers after grossing is complete.  Is anyone aware of any
recommendations, or does anyone out there retain their empty specimen
containers?

Rhonda Hartz
Technologist Supervisor
Anatomic Pathology Division
Saskatoon Health Region
(306) 655-8197
rhonda.hartz <@t> saskatoonhealthregion.ca

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 15
Date: Wed, 23 Jun 2010 09:06:14 +1000
From: Mia Woodruff <mia.woodruff <@t> qut.edu.au>
Subject: [Histonet] Oven for paraffin slide drying
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <E359F813FBA517409A67C9D78D3DA33D1CDE8E086A <@t> QUTEXMBX02.qut.edu.au>
Content-Type: text/plain; charset="us-ascii"

Hello all,

I need to purchase an oven for drying slides, on a very limited budget. I came across a "food dehydrator" which looks like an oven and has drawers which are the perfect size for the slide holders to fit into and it can reach a temperature of 60C - and it costs about 1/8 of the price of a normal oven.
Can anyone foresee any issues in me using this for drying paraffin slides? As far as I can tell there is a low air flow inside and the temp can be set up to 60 degrees so it seems perfect? Any comments?

Thanks
Mia











------------------------------

Message: 16
Date: Tue, 22 Jun 2010 19:34:09 -0400
From: Robert Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Help! In need of positive Gram Control (Jackie
        M       O'Connor)
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <AANLkTilL6oBernSPUNwB2_WmSNK1xYaAHm2gSuUN7XcQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

I agree - acute appendicitis with rupture and an abscess provides an
abundance of gram positive and gram negative bacteria, and such cases
are common.

I think that actual clinical tissue is better than controls faked with
reference organisms.

Tissue gram stains are greatly overrated. They don't work nearly as
well as smears do. If you want to see bacteria in tissue sections,
stain them with Giemsa or toluidine blue.

Bob Richmond
Samurai Pathologist
Knoxville TN



------------------------------

Message: 17
Date: Tue, 22 Jun 2010 20:16:41 -0400
From: Emily Sours <talulahgosh <@t> gmail.com>
Subject: Re: [Histonet] Oven for paraffin slide drying
To: Mia Woodruff <mia.woodruff <@t> qut.edu.au>,
        histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <AANLkTim_YBuPuQEzvGfWwe_QybhTj6oh2ivC5fHlLupu <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

Do slide warmers cost too much?
I don't know how much they cost, though, maybe an oven is cheaper.

Emily

Towns are like people. Old ones often have character, the new ones are
interchangeable.
--Wallace Stegner, Angle of Repose



On Tue, Jun 22, 2010 at 7:06 PM, Mia Woodruff <mia.woodruff <@t> qut.edu.au> wrote:
> Hello all,
>
> I need to purchase an oven for drying slides, on a very limited budget. I came across a "food dehydrator" which looks like an oven and has drawers which are the perfect size for the slide holders to fit into and it can reach a temperature of 60C - and it costs about 1/8 of the price of a normal oven.
> Can anyone foresee any issues in me using this for drying paraffin slides? As far as I can tell there is a low air flow inside and the temp can be set up to 60 degrees so it seems perfect? Any comments?
>
> Thanks
> Mia
>
>
>
>
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



------------------------------

Message: 18
Date: Tue, 22 Jun 2010 21:01:14 -0400
From: Komal Gada <kjgada <@t> gmail.com>
Subject: Re: [Histonet] Re: Amyloidosis control tissue
To: Robert Richmond <rsrichmond <@t> gmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <AANLkTinRMi6N2YhHijWg8ub3fkJmVxWzVOlUgm3jQWm_ <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

American mastertech

On Tue, Jun 22, 2010 at 12:19 PM, Robert Richmond <rsrichmond <@t> gmail.com>wrote:

> It's always been difficult to get amyloid control tissue. Amyloidosis
> is a rather rare disease in the USA, and some cases simply do not
> stain well.
>
> I've asked this question on Histonet several times and no one has ever
> responded: Amyloidosis (of the AA type I think) is rather easily
> produced in mice and other small experimental animals. Why can't this
> tissue be available as an amyloid control?
>
> Seems that some enterprising person could make a little money this way.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


------------------------------

Message: 19
Date: Tue, 22 Jun 2010 18:06:14 -0700
From: connie grubaugh <conniegrubaugh <@t> hotmail.com>
Subject: RE: [Histonet] Help! In need of positive Gram Control
To: <cgill <@t> marylandgeneral.org>, <jcbritton <@t> cheshire-med.com>,
        <dianarip1 <@t> aol.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT104-W16F336B53BE5A967C7507D6C50 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"


Tried the slim jim and all of my doctors did not like it.  Don't waste your time.



Connie G.





> Date: Tue, 22 Jun 2010 10:16:14 -0400
> From: cgill <@t> marylandgeneral.org
> To: JCBRITTON <@t> Cheshire-Med.COM; DianaRip1 <@t> aol.com; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Help! In need of positive Gram Control
> CC:
>
> You have got to be kidding!! That's hysterical. So process a slim jim
> and you have
> Gram - and + controls. If you're serious I'm trying it.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Josie
> Britton
> Sent: Tuesday, June 22, 2010 6:10 AM
> To: DianaRip1 <@t> aol.com; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Help! In need of positive Gram Control
>
>
>
>
>
> Have you tried a Slim Jim? They have gram positive and negative rods in
>
> them. Regardless, I still enjoy eating them once and a while!
>
>
>
>
>
>
>
> Josie Britton Ht
>
>
>
> Cheshire Medical Center
>
>
>
> Keene, NH 03431
>
>
>
>
>
>
>
>
>
>
>
> -----Original Message-----
>
> From: histonet-bounces <@t> lists.utsouthwestern.edu
>
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
>
> DianaRip1 <@t> aol.com
>
> Sent: Monday, June 21, 2010 7:43 PM
>
> To: histonet <@t> lists.utsouthwestern.edu
>
> Subject: [Histonet] Help! In need of positive Gram Control
>
>
>
>
>
>
>
> Help! We are in need of positive Gram Control Blocks if anyone has any
>
>
>
> extra they are willing to part with. I have lots of Fungus,
>
> Pneumocystis and
>
>
>
> HPV tissue blocks to trade.
>
>
>
>
>
>
>
> Diana Ripley
>
>
>
> John Muir Histology
>
>
>
> Concord Campus
>
>
>
> 2540 East Street
>
>
>
> Concord, CA 94520
>
>
>
> _______________________________________________
>
>
>
> Histonet mailing list
>
>
>
> Histonet <@t> lists.utsouthwestern.edu
>
>
>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> CONFIDENTIALITY NOTICE: This electronic message, including any
> attachments, is for the sole use of the intended recipients and may
> contain confidential and privileged information. Any unauthorized
> review, use, disclosure or distribution is prohibited. If you are not
> the intended recipient, please contact the sender by electronic mail and
> destroy all copies of the original message.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_________________________________________________________________
The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail.
http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5

------------------------------

Message: 20
Date: Tue, 22 Jun 2010 21:50:42 EDT
From: JMyers1 <@t> aol.com
Subject: RE: [Histonet] New CAP question ANP.22760
To: tjasper <@t> copc.net
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <7cdd9.6a7042fa.3952c272 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"

Tom:

As much as I agree with your acknowledgment that its seems a bit odd for
the CAP to have a blood-banker responding to AP-related issue, I'm actually
not surprised.  The folks in the 'clinical' lab have been performing more
comprehensive and complex validation procedures for a very long time, and they
wonder why IHC isn't expected to follow the same requirements as chemistry,
immunology, etc. -- IHC is, after all, an awful lot like ELISA.  And
rightfully so, because IHC is, under CLIA (which supersedes CAP), considered
highly-complex, non-waived testing -- and is, therefore, subject to the same
Quality Systems regulations (in particular, 42CFR493.1252-1256, 1273, and 1281) as
the testing performed in other areas of the lab.

Could it be that, because AP produces qualitative results that are
interpreted by a pathologist and CP produces quantitative results that are
interpreted by an analyzer, we somehow think that CLIA rules don't apply to IHC?  I
certainly don't have the answer to that, but it make me wonder what the
future holds.  As witnessed by some of the newest CAP 'standards' (including the
question in question...no pun intended), e.g. ER/PR, where a minimum of 20
positive and 20 negative specimens must be tested, and where 10 of the
positives must be weakly positive -- an acknowledgment that validation specimens
must be carefully selected in order to obtain appropriate results), it
certainly doesn't appear that the regulation of IHC testing is going to become
more relaxed.

Joe Myers, M.S., CT(ASCP)

------------------------------

Message: 12
Date: Fri, 18 Jun 2010 12:38:07 -0700
From: "Thomas Jasper" <tjasper <@t> copc.net>
Subject: RE: [Histonet] New CAP question ANP.22760
To: "Mark Tarango" <marktarango <@t> gmail.com>
Cc: _histonet <@t> lists.utsouthwestern.edu_
(mailto:histonet <@t> lists.utsouthwestern.edu)

Mark,

Did you notice the credentials from this CAP representative? MT with a
Blood Bank specialty I believe.  What I glean from that is...more than
likely this person does not grasp the logistics of "contemporaneously"
staining identical Abs from separate lots.  She also likely does not
understand the logistical application for detection and automation
either.

I'm not trying to be overly critical of this person.  I'm sure she is
quite intelligent and would not have the MT/SBB if she wasn't
intelligent.  It comes down to a lack of understanding Anatomic
Pathology testing application re: automated IHC.  I believe this is a
common problem in and out of CAP. Many lab directors and other folks in
positions of authority without AP/Histology/Cytology backgrounds seem to
believe that broad clinical lab modalities apply to Anatomic Path
scenarios.  I used to refer to this in my former position as - "Trying
to put the yoke of clinical lab onto anatomic path."  We are
laboratorians, but in many instances do not fit the general clinical lab
mold.

It's unfortunate that CAP has put this person in the position to
respond.  It is apparent to me that she's not grasping the particulars
here.  She probably never will unless she decides to go into a working,
automated IHC "tissue" lab and take the time to ask questions and
understand (learn) what we're all about.

Thanks,
Tom Jasper

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, OR 97701


------------------------------

Message: 21
Date: Wed, 23 Jun 2010 02:14:47 -0500
From: <Susan.Walzer <@t> HCAHealthcare.com>
Subject: RE: [Histonet] Help! In need of positive Gram Control
To: <conniegrubaugh <@t> hotmail.com>, <cgill <@t> marylandgeneral.org>,
        <jcbritton <@t> cheshire-med.com>, <dianarip1 <@t> aol.com>,
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <4BF03F5404EBDE409AF9232DA74B9DED2AF9784613 <@t> FWDCWPMSGCMS09.hca.corpad.net>

Content-Type: text/plain; charset="us-ascii"

Take some fresh tissue (we use umbilical cord)  to micro and let them incubate in for a few days in gram + and/or negative. Then fix it and you will have good controls.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of connie grubaugh
Sent: Tuesday, June 22, 2010 9:06 PM
To: cgill <@t> marylandgeneral.org; jcbritton <@t> cheshire-med.com; dianarip1 <@t> aol.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Help! In need of positive Gram Control


Tried the slim jim and all of my doctors did not like it.  Don't waste your time.



Connie G.





> Date: Tue, 22 Jun 2010 10:16:14 -0400
> From: cgill <@t> marylandgeneral.org
> To: JCBRITTON <@t> Cheshire-Med.COM; DianaRip1 <@t> aol.com; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Help! In need of positive Gram Control
> CC:
>
> You have got to be kidding!! That's hysterical. So process a slim jim
> and you have
> Gram - and + controls. If you're serious I'm trying it.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Josie
> Britton
> Sent: Tuesday, June 22, 2010 6:10 AM
> To: DianaRip1 <@t> aol.com; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Help! In need of positive Gram Control
>
>
>
>
>
> Have you tried a Slim Jim? They have gram positive and negative rods in
>
> them. Regardless, I still enjoy eating them once and a while!
>
>
>
>
>
>
>
> Josie Britton Ht
>
>
>
> Cheshire Medical Center
>
>
>
> Keene, NH 03431
>
>
>
>
>
>
>
>
>
>
>
> -----Original Message-----
>
> From: histonet-bounces <@t> lists.utsouthwestern.edu
>
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
>
> DianaRip1 <@t> aol.com
>
> Sent: Monday, June 21, 2010 7:43 PM
>
> To: histonet <@t> lists.utsouthwestern.edu
>
> Subject: [Histonet] Help! In need of positive Gram Control
>
>
>
>
>
>
>
> Help! We are in need of positive Gram Control Blocks if anyone has any
>
>
>
> extra they are willing to part with. I have lots of Fungus,
>
> Pneumocystis and
>
>
>
> HPV tissue blocks to trade.
>
>
>
>
>
>
>
> Diana Ripley
>
>
>
> John Muir Histology
>
>
>
> Concord Campus
>
>
>
> 2540 East Street
>
>
>
> Concord, CA 94520
>
>
>
> _______________________________________________
>
>
>
> Histonet mailing list
>
>
>
> Histonet <@t> lists.utsouthwestern.edu
>
>
>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> CONFIDENTIALITY NOTICE: This electronic message, including any
> attachments, is for the sole use of the intended recipients and may
> contain confidential and privileged information. Any unauthorized
> review, use, disclosure or distribution is prohibited. If you are not
> the intended recipient, please contact the sender by electronic mail and
> destroy all copies of the original message.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_________________________________________________________________
The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail.
http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 22
Date: Wed, 23 Jun 2010 10:24:22 +0200
From: "Hoekert, W.E.J." <W.E.J.Hoekert <@t> olvg.nl>
Subject: RE: [Histonet] Glut1 antibody
To: "Martha Ward" <mward <@t> wfubmc.edu>,
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1190CB05C44B13409483514729C2FC360C0ADF <@t> PAIT42.olvg.nl>
Content-Type: text/plain;       charset="iso-8859-1"

We switched to Neomarkers (polyclonal) (RB 078-A).

Willem Hoekert
OLVG, Netherlands

________________________________

Van: histonet-bounces <@t> lists.utsouthwestern.edu namens Martha Ward
Verzonden: di 22-6-2010 22:10
Aan: histonet <@t> lists.utsouthwestern.edu
Onderwerp: [Histonet] Glut1 antibody



I have been using anti-human Glut-1 from Dako for several years but when
I went to order it I was told it was discontinued.  Does anyone have a
suggestion of where I can locate this antibody?  Thanks in advance for
your help!

Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
336-716-2104



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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Message: 23
Date: Wed, 23 Jun 2010 08:25:33 -0400
From: mtitford <@t> aol.com
Subject: [Histonet] Retaining empty jars
To: Histonet <@t> pathology.swmed.edu
Message-ID: <8CCE0E64F6A9C4A-FCC-6F33 <@t> Webmail-d119.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"


Rhonda Hartz asks if anyone else retains empty jars after grossing.

We too retain our empty jars after grossing for about a month. We keep them in a red biohazard trash bag. It pays dividends. If once the case has been signed out the physician/surgeon complains that they removed more biopsies than we listed in the report, or submitted more jars, etc, we can go back and check. On rare occasions we have found tiny pieces of tissue in the jars, or hidden in the grooves of the lid. These enquiries most often happen with tiny pieces of tissue, of course, like GI biopsies.

 We have the physicians write on the label on the jar what is inside it (like everyone else I guess) as well as listing it on the surgical pathology slip. You can also go back and check what they wrote down.

Afterwards the jars are incinerated as they have all that HIPPA related stuff on the labels.

Michael Titford
USA Pathology
Mobile AL USA





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End of Histonet Digest, Vol 79, Issue 28
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