[Histonet] cryojane question

[Sebree Linda A]

I've used it for serial frozen sections in the past; worked very well
for that application. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Emily
Sours
Sent: Friday, June 18, 2010 9:10 AM
To: Kim Merriam; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] cryojane question

Histonetters,

I just looked up what a cryojane was, and it's pretty neat!
Does anyone else use this?
The one flaw seems to be that you can only put one section on a slide
(or at least that the way it's depicted here:
http://www.instrumedics.com/cryojanetapetransferprocess.htm ) which
makes it pretty time consuming.  Also does the uv step interfere with in
situ protocols? I guess not since the DNA/RNA is already transcribed and
fixed and therefore wouldn't be mutated.


Emily


Towns are like people. Old ones often have character, the new ones are
interchangeable.
--Wallace Stegner, Angle of Repose
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